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Literature summary for 3.5.4.38 extracted from

  • Adolph, M.B.; Love, R.P.; Feng, Y.; Chelico, L.
    Enzyme cycling contributes to efficient induction of genome mutagenesis by the cytidine deaminase APOBEC3B (2017), Nucleic Acids Res., 45, 11925-11940 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.38

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 dimeric enzyme form, APOBEC3B predominantly forms tetramers and a small proportion existed as dimers, gel filtration Homo sapiens
184000
-
 tetrameric enzyme form, APOBEC3B predominantly forms tetramers and a small proportion existed as dimers, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine in single-stranded DNA + H2O Homo sapiens the enzymes must access single-stranded DNA during the dynamic processes of DNA replication or transcription. The ability of an APOBEC3 to cycle between DNA substrates determines whether it is able to efficiently deaminate single-stranded DNA produced by replication and single-stranded DNA bound by replication protein A. APOBEC3 deaminase activity during transcription has a size limitation that inhibits APOBEC3B tetramers uracil in single-stranded DNA + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UH17
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine in single-stranded DNA + H2O
-
 Homo sapiens uracil in single-stranded DNA + NH3
-
 ?
cytosine in single-stranded DNA + H2O the enzymes must access single-stranded DNA during the dynamic processes of DNA replication or transcription. The ability of an APOBEC3 to cycle between DNA substrates determines whether it is able to efficiently deaminate single-stranded DNA produced by replication and single-stranded DNA bound by replication protein A. APOBEC3 deaminase activity during transcription has a size limitation that inhibits APOBEC3B tetramers Homo sapiens uracil in single-stranded DNA + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE, APOBEC3B predominantly forms tetramers and a small proportion existed as dimers Homo sapiens
tetramer 4 * 46000, SDS-PAGE, APOBEC3B predominantly forms tetramers and a small proportion existed as dimers Homo sapiens

Synonyms

Synonyms Comment Organism
APOBEC3B
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme can contribute to cancer through deamination of cytosine to form promutagenic uracil in genomic DNA Homo sapiens