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Literature summary for 3.5.4.35 extracted from

  • Randau, L.; Stanley, B.; Kohlway, A.; Mechta, S.; Xiong, Y.; Soell, D.
    A cytidine deaminase edits C to U in transfer RNAs in archaea (2009), Science, 324, 657-659.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanopyrus kandleri

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion. The structure of the enzyme is solved in two different crystal forms. In each form, the asymmetric unit is composed of two identical CDAT8 dimers Methanopyrus kandleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
2 * 30600 Methanopyrus kandleri
60000
-
gel filtration Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine8 in tRNA + H2O Methanopyrus kandleri all canonical tRNAs have a uridine at position 8, involved in maintaining tRNA tertiary structure. The hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. The enzyme catalyzes C-to-U editing at this location. The presence of this C-to-U editing enzyme guarantees the proper folding and functionality of all Methanopyrus kandleri tRNAs uracil8 in tRNA + NH3
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?

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri Q8TWU6
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine8 in tRNA + H2O all canonical tRNAs have a uridine at position 8, involved in maintaining tRNA tertiary structure. The hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. The enzyme catalyzes C-to-U editing at this location. The presence of this C-to-U editing enzyme guarantees the proper folding and functionality of all Methanopyrus kandleri tRNAs Methanopyrus kandleri uracil8 in tRNA + NH3
-
?
cytosine8 in tRNA + H2O the enzyme is specific for C deamination at position 8, requires only the acceptor stem hairpin for activity. CDAT8 is able to recognize 30 different tRNAs mainly by interacting with the tRNA acceptor stem, including the universal 3'-CCA end, and a flexible base C8 Methanopyrus kandleri uracil8 in tRNA + NH3
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?

Subunits

Subunits Comment Organism
dimer 2 * 30600 Methanopyrus kandleri

Synonyms

Synonyms Comment Organism
CDAT8
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Methanopyrus kandleri
MK0935
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Methanopyrus kandleri

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
70
-
assay at Methanopyrus kandleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Methanopyrus kandleri