BRENDA - Enzyme Database show
show all sequences of 3.5.4.23

Substrate binding by blasticidin S deaminase, an aminohydrolase for novel 4-aminopyrimidine nucleosides

Yamaguchi, I.; Sheto, H.; Misato, T.; Pestic. Biochem. Physiol. 25, 54-62 (1986)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Gougerotin
enzyme induced by
Aspergillus terreus
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-deaminohydroxycytomycin
competitive inhibition
Aspergillus terreus
acetylcytosinine
competitive inhibition
Aspergillus terreus
acetyldeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
caproylcytosinine
competitive inhibition
Aspergillus terreus
cytosinine
competitive inhibition
Aspergillus terreus
deaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
deaminohydroxyblasticidin S methylester
-
Aspergillus terreus
deaminomercaptoblasticidin S
competitive inhibition
Aspergillus terreus
dihydrocytomycin
competitive inhibition
Aspergillus terreus
dihydrodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
uracinine
competitive inhibition
Aspergillus terreus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
blasticidin S
-
Aspergillus terreus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
blasticidin S + H2O
Bacillus sp. (in: Bacteria)
-
deaminohydroxyblasticidin S + NH3
-
-
?
blasticidin S + H2O
Streptomyces sp.
-
deaminohydroxyblasticidin S + NH3
-
-
?
blasticidin S + H2O
Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
-
?
blasticidin S + H2O
Aspergillus fumigatus
-
deaminohydroxyblasticidin S + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Aspergillus terreus
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Streptomyces sp.
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus terreus
Storage Stability
Storage Stability
Organism
-20°C, 40% glycerol-5 mM Tris-HCl buffer, pH 7.5, 5 mM 2-mercaptoethanol, no detectable loss of activity over a period of 3 months
Aspergillus terreus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetylblasticidin S + H2O
-
209570
Aspergillus terreus
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
alanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
blasticidin S + H2O
-
209570
Bacillus sp. (in: Bacteria)
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S + H2O
-
209570
Streptomyces sp.
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S + H2O
-
209570
Aspergillus terreus
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S + H2O
-
209570
Aspergillus fumigatus
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S methylester + H2O
only slightly deaminated
209570
Aspergillus terreus
deaminohydroxyblasticidin S methylester + NH3
-
-
-
?
cytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
cytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
cytosinine + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
demethylblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
deoxycytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
dihydroblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
dihydrocytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
gougerotin + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
additional information
cytosine, CMP, d-CMP, adenine, guanine and their nucleosides and nucleotides are no substrates
209570
Aspergillus terreus
?
-
-
-
-
phenylalanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
pyrimidinoblasticidin S + H2O
-
209570
Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S + NH3
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Gougerotin
enzyme induced by
Aspergillus terreus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-deaminohydroxycytomycin
competitive inhibition
Aspergillus terreus
acetylcytosinine
competitive inhibition
Aspergillus terreus
acetyldeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
caproylcytosinine
competitive inhibition
Aspergillus terreus
cytosinine
competitive inhibition
Aspergillus terreus
deaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
deaminohydroxyblasticidin S methylester
-
Aspergillus terreus
deaminomercaptoblasticidin S
competitive inhibition
Aspergillus terreus
dihydrocytomycin
competitive inhibition
Aspergillus terreus
dihydrodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
uracinine
competitive inhibition
Aspergillus terreus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
blasticidin S
-
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
blasticidin S + H2O
Bacillus sp. (in: Bacteria)
-
deaminohydroxyblasticidin S + NH3
-
-
?
blasticidin S + H2O
Streptomyces sp.
-
deaminohydroxyblasticidin S + NH3
-
-
?
blasticidin S + H2O
Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
-
?
blasticidin S + H2O
Aspergillus fumigatus
-
deaminohydroxyblasticidin S + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus terreus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 40% glycerol-5 mM Tris-HCl buffer, pH 7.5, 5 mM 2-mercaptoethanol, no detectable loss of activity over a period of 3 months
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetylblasticidin S + H2O
-
209570
Aspergillus terreus
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
alanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
blasticidin S + H2O
-
209570
Bacillus sp. (in: Bacteria)
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S + H2O
-
209570
Streptomyces sp.
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S + H2O
-
209570
Aspergillus terreus
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S + H2O
-
209570
Aspergillus fumigatus
deaminohydroxyblasticidin S + NH3
-
-
-
?
blasticidin S methylester + H2O
only slightly deaminated
209570
Aspergillus terreus
deaminohydroxyblasticidin S methylester + NH3
-
-
-
?
cytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
cytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
cytosinine + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
demethylblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
deoxycytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
dihydroblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
dihydrocytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
gougerotin + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
additional information
cytosine, CMP, d-CMP, adenine, guanine and their nucleosides and nucleotides are no substrates
209570
Aspergillus terreus
?
-
-
-
-
phenylalanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
pyrimidinoblasticidin S + H2O
-
209570
Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S + NH3
-
-
-
?
Other publictions for EC 3.5.4.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733166
Li
Streptomyces lividans blastici ...
Streptomyces lividans, Streptomyces lividans HXY16
Appl. Environ. Microbiol.
79
2349-2357
2013
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1
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4
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1
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8
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1
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1
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8
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697727
Bugorsky
Genetic selection system allow ...
Aspergillus terreus
Eur. J. Histochem.
52
1-10
2008
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1
1
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4
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687659
Kumasaka
Crystal structures of blastici ...
Aspergillus terreus, Aspergillus terreus S-712 / ATCC 28865
J. Biol. Chem.
282
37103-37111
2007
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1
1
3
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1
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4
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1
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1
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1
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3
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1
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1
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1
1
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654417
Kimura
The flexible C-terminal region ...
Aspergillus terreus
Biochem. Biophys. Res. Commun.
290
421-426
2002
-
-
1
-
1
-
1
4
-
1
-
-
-
2
-
-
-
1
1
-
-
-
-
2
-
-
1
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
6
-
-
-
-
-
-
-
-
-
-
209578
Kimura
Expression, purification, and ...
Aspergillus terreus, Bacillus cereus
J. Biochem.
127
955-963
2000
-
-
1
1
5
1
2
-
-
1
6
2
1
3
-
-
1
1
1
-
-
-
2
2
-
-
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1
-
1
5
1
-
2
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1
6
2
1
-
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1
1
-
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-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209576
Nakasako
Crystallization and preliminar ...
Aspergillus terreus
Acta Crystallogr. Sect. D
55
547-548
1999
-
-
1
1
-
-
-
-
-
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1
-
2
-
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-
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1
1
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1
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1
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1
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1
1
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-
209577
Fukuda
-
A new transformation system of ...
Aspergillus terreus
Biotechnol. Lett.
21
969-971
1999
-
-
1
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-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
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-
-
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-
-
209575
Karremann
New positive/negative selectab ...
Aspergillus terreus
Nucleic Acids Res.
26
2508-2510
1998
-
-
1
-
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-
-
-
-
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1
-
1
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1
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1
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1
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1
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-
-
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-
-
-
-
209574
Tamura
Blasticidin S deaminase gene(B ...
Aspergillus terreus
Biosci. Biotechnol. Biochem.
59
2336-2338
1995
-
1
1
-
-
-
-
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1
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5
-
-
1
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1
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1
1
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1
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1
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1
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-
-
-
-
-
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-
209573
Kimura
Cloning of the blasticidin S d ...
Aspergillus terreus, Bacillus sp. (in: Bacteria)
Mol. Gen. Genet.
242
121-129
1994
1
1
2
-
-
-
-
1
-
-
1
2
-
11
-
-
-
-
-
-
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2
1
-
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-
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1
1
2
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1
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1
2
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-
2
1
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-
-
-
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209571
Endo
Inactivation of blasticidin S ...
Bacillus cereus, Bacillus cereus K55-S1, Bacillus cereus K55-S2
J. Antibiot.
40
1791-1793
1987
-
-
-
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3
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4
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9
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1
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1
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3
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9
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1
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1
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209572
Kamakura
-
Cloning and expression of a ne ...
Bacillus cereus, Bacillus cereus K55-S1
Agric. Biol. Chem.
51
3165-3168
1987
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1
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2
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3
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2
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1
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2
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2
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-
-
-
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-
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-
-
209570
Yamaguchi
-
Substrate binding by blasticid ...
Aspergillus fumigatus, Aspergillus terreus, Bacillus sp. (in: Bacteria), Streptomyces sp.
Pestic. Biochem. Physiol.
25
54-62
1986
1
-
-
-
-
-
12
1
-
-
-
4
-
4
-
-
1
-
-
-
-
1
18
-
-
-
-
-
-
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-
-
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1
-
-
-
-
-
-
-
12
-
1
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-
4
-
-
-
1
-
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-
1
18
-
-
-
-
-
-
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-
-
-
-
209569
Yamaguchi
-
Active center and mode of reac ...
Aspergillus terreus
Agric. Biol. Chem.
49
3355-3361
1985
1
-
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1
2
2
-
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-
1
-
1
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1
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5
-
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1
1
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1
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1
-
2
-
2
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1
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1
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5
-
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-
1
1
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-
-
-
-
-
209567
Yamaguchi
Isolation and purification of ...
Aspergillus flavipes, Aspergillus flavipes NHL 5013, Aspergillus flavus, Aspergillus fumigatus, Aspergillus fumigatus NHL 5009, Aspergillus ochraceus, Aspergillus ochraceus 67-MI-87, Aspergillus terreus, Aspergillus terreus NHL 5037, Aspergillus ustus 1004, Aspergillus ustus
J. Antibiot.
28
7-14
1975
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21
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11
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21
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1
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1
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209568
Misato
-
Blasticidin S deaminase produc ...
Aspergillus terreus
Jpn. Kokai JP48099383 JP72-33359
19 pp
1973
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