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Literature summary for 3.5.4.23 extracted from

  • Yamaguchi, I.; Sheto, H.; Misato, T.
    Substrate binding by blasticidin S deaminase, an aminohydrolase for novel 4-aminopyrimidine nucleosides (1986), Pestic. Biochem. Physiol., 25, 54-62.
No PubMed abstract available
Search for more literature for 3.5.4.23

Activating Compound

Activating Compound Comment Organism Structure
Gougerotin enzyme induced by Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
4-deaminohydroxycytomycin competitive inhibition Aspergillus terreus
acetylcytosinine competitive inhibition Aspergillus terreus
acetyldeaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
caproylcytosinine competitive inhibition Aspergillus terreus
cytosinine competitive inhibition Aspergillus terreus
deaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
deaminohydroxyblasticidin S methylester
-
 Aspergillus terreus
deaminomercaptoblasticidin S competitive inhibition Aspergillus terreus
dihydrocytomycin competitive inhibition Aspergillus terreus
dihydrodeaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
uracinine competitive inhibition Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 blasticidin S
-
 Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
blasticidin S + H2O Bacillus sp. (in: Bacteria)
-
 deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O Streptomyces sp.
-
 deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O Aspergillus terreus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O Aspergillus fumigatus
-
 deaminohydroxyblasticidin S + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus terreus
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus terreus

Storage Stability

Storage Stability Organism
-20°C, 40% glycerol-5 mM Tris-HCl buffer, pH 7.5, 5 mM 2-mercaptoethanol, no detectable loss of activity over a period of 3 months Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylblasticidin S + H2O
-
 Aspergillus terreus acetyldeaminohydroxyblasticidin S + NH3
-
 ?
alanylcytosinine + H2O
-
 Aspergillus terreus ?
-
 ?
blasticidin S + H2O
-
 Bacillus sp. (in: Bacteria) deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O
-
 Streptomyces sp. deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O
-
 Aspergillus fumigatus deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S methylester + H2O only slightly deaminated Aspergillus terreus deaminohydroxyblasticidin S methylester + NH3
-
 ?
cytidine + H2O
-
 Aspergillus terreus ?
-
 ?
cytomycin + H2O
-
 Aspergillus terreus ?
-
 ?
cytosinine + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
demethylblasticidin S + H2O
-
 Aspergillus terreus ?
-
 ?
deoxycytidine + H2O
-
 Aspergillus terreus ?
-
 ?
dihydroblasticidin S + H2O
-
 Aspergillus terreus ?
-
 ?
dihydrocytomycin + H2O
-
 Aspergillus terreus ?
-
 ?
gougerotin + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
additional information cytosine, CMP, d-CMP, adenine, guanine and their nucleosides and nucleotides are no substrates Aspergillus terreus ?
-
 ?
phenylalanylcytosinine + H2O
-
 Aspergillus terreus ?
-
 ?
pyrimidinoblasticidin S + H2O
-
 Aspergillus terreus pyrimidinodeaminohydroxyblasticidin S + NH3
-
 ?