A model of the incidence and consequences of choroidal neovascularization secondary to age-related macular degeneration. Comparative effects of current treatment and potential prophylaxis on visual outcomes in high-risk patients.
generating of bicistronic vectors express both blastidicin S deaminase gene and a fusion gene consisting of a sarcomeric protein, selection with blasticidin S results in a relatively pure population of ES cell-derived cardiomycetes
PCR amplification of the BSD gene, cloned from cDNA and expressed in Escherichia coli, also transformed into Schizosaccharomyces pombe and Pyricularia oryzae, P. oryzae could not be transformed with bsr, but with BSD
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reconstitution of the enzyme after denaturation with guanidine-HCl, acid, or p-hydroxymercuriphenylsulfonic acid, using different divalent metal ions, renaturation of the enzyme is completely blocked in presence of 1 mM EDTA, refolding of recombinant Cys91 mutant enzymes from inclusion bodies
wild-type and deletion mutant apoenzymes, prepared by zinc removal via EDTA, exhibit similar physical properties in thermodynamic refolding into the stable tetramer conformation, reconstitution with zinc
the enzyme is a selection marker for genetic transformation of the diatom Phaeodactylum tricornutum. An easy handle system of antibiotic selection with blasticidin-S and the resistance gene bsr in Phaeodactylum tricornutum is reported. Blasticidin-S works by blocking protein translation and therefore it is not expected to affect the genomic DNA of transformed cells. Especially for reverse genetics studies via genome editing, when phenotypes should be determined based on cell-linages with as less off-target genetic changes as possible, utilization of this antibiotic is a major improvement. Additionally, blasticidin-S selection can be used in combination with nourseothricin- and potentially also with Zeocin-resistant cells. This extension of the genetic toolbox can be useful for further molecular characterization or biotechnological application of the model diatom Phaeodactylum tricornutum and possibly for other species of diatoms
BSD gene will be useful as a new dominant selectable marker for eukaryotes, first sucessful transformation with a drug resistance gene originating from a eukaryote by selecting for detoxification of the drug