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Literature summary for 3.5.4.1 extracted from

  • Mallano, A.; Zamboni, S.; Carpinelli, G.; Santoro, F.; Flego, M.; Ascione, A.; Gellini, M.; Tombesi, M.; Podo, F.; Cianfriglia, M.
    Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from yeast (2008), BMC Biotechnol., 8, 68.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.1

Application

Application Comment Organism
pharmacology cancer chemotherapy, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), biopanning assay Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli TG1, pQE30Xa plasmid Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-fluorocytosine + H2O Saccharomyces cerevisiae recombinant protein of cytosine deaminase used as antigen for biopanning approach, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), binding of specific single chain fragment variable (scFv) with cytosine deaminase does not interfere with enzyme activity 5-fluorouracil + NH3
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12178 recombinant protein
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Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 selection and characterization of a human monoclonal antibody in single chain fragment (scFv) format for detection of expression of cytosine deaminase in GDEPT/ADEPT studies, selection and characterization of specific single chain fragment variable (scFv) antibody specific for cytosine deaminase, cytotoxic assay, amino acid sequence of the CDR3 regions of the selected antibody and genetic structure of phage antibody from ETH-2 library determined, human antibody generated by this method to detect cytosine deaminase in routinary laboratory techniques without interfering with its enzymatic function Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O recombinant protein of cytosine deaminase used as antigen for biopanning approach, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), binding of specific single chain fragment variable (scFv) with cytosine deaminase does not interfere with enzyme activity Saccharomyces cerevisiae 5-fluorouracil + NH3
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 ?

Synonyms

Synonyms Comment Organism
yCD
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 Saccharomyces cerevisiae