Application | Comment | Organism |
---|---|---|
pharmacology | the recombinant fusion enzyme HSV-1TKglyCD might be useful in cancer gene therapy | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of the fusion protein HSV-1TKglyCD in HEK-293 cells and in the human lung cancer cell line NCIH460 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a fusion protein HSV-1TKglyCD combining Herpes simplex virus type-1 thymidine kinase, HSV-1TK, and Escherichia coli cytosine deaminase, CD, with screening for the optimal suitable linker peptide, molecular modeling, overview | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ganciclovir | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
recombinant fusion protein HSV-1TKglyCD | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | Escherichia coli | recombinant fusion enzyme, cytotoxicity could be enhanced by concurrently treating TKglyCD-expressing cells with prodrugs ganclocivir and 5-fluorocytosine, overview | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25524 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
5-fluorocytosine + H2O | recombinant fusion enzyme, cytotoxicity could be enhanced by concurrently treating TKglyCD-expressing cells with prodrugs ganclocivir and 5-fluorocytosine, overview | Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | recombinant fusion protein HSV-1TKglyCD, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.6 | - |
37°C, recombinant fusion protein HSV-1TKglyCD | Escherichia coli | ganciclovir |