Protein Variants | Comment | Organism |
---|---|---|
additional information | co-expression of the uracil phosphoribosyltransferase gene with a chimeric truncated human nerve growth factor receptor/cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosine, for positive selection, overview, construct NG/CDiU expressing UPRT and NG/CD, using a bicistronic message, provides the greatest UPRT activity and killing, reducing the lethal dose of 5-FC sufficient to eradicate 90% of cells | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | activation of the prodrug, the cytosine deaminase gene is an alternative gene for negative selection producing the toxic metabolite 5-fluorouracil, sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase, which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? |