Literature summary for 3.5.1.92 extracted from

Boersma, Y.L.; Newman, J.; Adams, T.E.; Cowieson, N.; Krippner, G.; Bozaoglu, K.; Peat, T.S.
The structure of vanin 1: a key enzyme linking metabolic disease and inflammation (2014), Acta Crystallogr. Sect. D, 70, 3320-3329.

Cloned(Commentary)

Cloned (Comment)	Organism
transient expression of soluble FLAG-tagged truncated enzyme mutant, and mutant enzymes E249Q and E439Q in HEK-293T cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant FLAG-tagged truncated enzyme, and enzyme mutants E249Q and E439Q, free and in complex with pantothenic acid or inhibitor RR6, sitting drop vapor diffusion method, mixing of 200 nl of 12 mg/ml kifunensine-treated vanin 1 protein in 50 mM bis-tris pH 6.5, 50 mM NaCl, with 200 nl of reservoir solution containing 21% w/v PEG 1500, 10% v/v malate-MES-Tris, pH 6.3, with or without 1 mM pantothenic acid or inhibitor RR6, 25°C, 40-50 days, or 4 weeks after microseeding, X-ray diffraction structure determination and analysis at 2.25 A resolution, molecular replacement and modeling	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant FLAG-tagged truncated enzyme, and enzyme mutants E249Q and E439Q, free and in complex with pantothenic acid or inhibitor RR6, sitting drop vapor diffusion method, mixing of 200 nl of 12 mg/ml kifunensine-treated vanin 1 protein in 50 mM bis-tris pH 6.5, 50 mM NaCl, with 200 nl of reservoir solution containing 21% w/v PEG 1500, 10% v/v malate-MES-Tris, pH 6.3, with or without 1 mM pantothenic acid or inhibitor RR6, 25°C, 40-50 days, or 4 weeks after microseeding, X-ray diffraction structure determination and analysis at 2.25 A resolution, molecular replacement and modeling

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E249Q	site-directed mutagenesis	Homo sapiens
E439Q	site-directed mutagenesis	Homo sapiens
E79A	site-directed mutagenesis	Homo sapiens
K178A	site-directed mutagenesis	Homo sapiens
additional information	construction of a truncated mutant comprising amino acids 22-493, deleting the native signal sequence and the C-terminal GPI-anchor sequence, and cloning downstream of the mouse interleukin-3 signal peptide in the mammalian expression vector pAPEX-3P and a FLAG tag DYDDDDK	Homo sapiens
T26I	naturally occuring variant	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
(R)-2,4-dihydroxy-3,3-dimethyl-N-(3-oxo-4-phenylbutyl)butanamide	i.e. RR6	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell surface	a glycosylphosphatidylinositol (GPI)-anchored ectoenzyme	Homo sapiens	9986	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-pantetheine + H2O	Homo sapiens	-	(R)-pantothenate + 2-aminoethanethiol	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O95497	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycolipoprotein	a glycosylphosphatidylinositol (GPI)-anchored ectoenzyme	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble FLAG-tagged truncated enzyme mutant from 293 cells by immunoaffinity chromatography and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-pantetheine + H2O	-	Homo sapiens	(R)-pantothenate + 2-aminoethanethiol	-	?
N-(4-methyl-2-oxo-2H-chromen-7-yl)-D-pantothenamide + H2O	-	Homo sapiens	(R)-pantothenate + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
oligomer	the residues important for oligomerization are Gln179-Phe182, Pro191-Glu193, Phe212-Asp218, Pro243-His251 and Leu326-Asn330	Homo sapiens

Synonyms

Synonyms	Comment	Organism
pantetheinase	-	Homo sapiens
vanin 1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	key enzyme linking metabolic disease and inflammation	Homo sapiens