BRENDA - Enzyme Database show
show all sequences of 3.5.1.65

Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products

Soda, K.; Uchiyama, K.; Ogata, K.; Agric. Biol. Chem. 30, 541-546 (1966)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
D-glutamate
activation and induction
Pseudomonas aeruginosa
L-glutamate
activation and induction
Pseudomonas aeruginosa
L-Theanine
activation and induction
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N5-ethyl-L-glutamine + H2O
Pseudomonas aeruginosa
N-ethyl-gamma-L-glutamine
L-glutamate + ethylamine
-
Pseudomonas aeruginosa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas sp.
-
T-1
-
Pseudomonas sp. T-1
-
T-1
-
Purification (Commentary)
Commentary
Organism
partial, glutaminase could not be separated
Pseudomonas aeruginosa
Reaction
Reaction
Commentary
Organism
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
Pseudomonas aeruginosa
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
Pseudomonas fluorescens
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas fluorescens
L-glutamate + ethylamine
-
209220
Pseudomonas fluorescens
?
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas aeruginosa
L-glutamate + ethylamine
-
209220
Pseudomonas aeruginosa
?
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas sp.
L-glutamate + ethylamine
-
209220
Pseudomonas sp.
?
N5-ethyl-L-glutamine + H2O
N-ethyl-gamma-L-glutamine
209220
Pseudomonas aeruginosa
L-glutamate + ethylamine
-
209220
Pseudomonas aeruginosa
?
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas sp. T-1
L-glutamate + ethylamine
-
209220
Pseudomonas sp. T-1
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Pseudomonas aeruginosa
37
-
assay at
Pseudomonas fluorescens
37
-
assay at
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas aeruginosa
8
-
assay at
Pseudomonas fluorescens
8
-
assay at
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
D-glutamate
activation and induction
Pseudomonas aeruginosa
L-glutamate
activation and induction
Pseudomonas aeruginosa
L-Theanine
activation and induction
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N5-ethyl-L-glutamine + H2O
Pseudomonas aeruginosa
N-ethyl-gamma-L-glutamine
L-glutamate + ethylamine
-
Pseudomonas aeruginosa
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial, glutaminase could not be separated
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas fluorescens
L-glutamate + ethylamine
-
209220
Pseudomonas fluorescens
?
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas aeruginosa
L-glutamate + ethylamine
-
209220
Pseudomonas aeruginosa
?
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas sp.
L-glutamate + ethylamine
-
209220
Pseudomonas sp.
?
N5-ethyl-L-glutamine + H2O
N-ethyl-gamma-L-glutamine
209220
Pseudomonas aeruginosa
L-glutamate + ethylamine
-
209220
Pseudomonas aeruginosa
?
N5-ethyl-L-glutamine + H2O
-
209220
Pseudomonas sp. T-1
L-glutamate + ethylamine
-
209220
Pseudomonas sp. T-1
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Pseudomonas aeruginosa
37
-
assay at
Pseudomonas fluorescens
37
-
assay at
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas aeruginosa
8
-
assay at
Pseudomonas fluorescens
8
-
assay at
Pseudomonas sp.
Other publictions for EC 3.5.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209219
Tsushida
-
Metabolism of L-theanine in te ...
Camellia sinensis
Jpn. Agric. Res. Q
21
42-46
1987
1
-
-
-
-
1
2
-
-
1
-
2
-
1
-
-
1
-
-
1
1
-
10
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
-
-
1
-
2
-
-
-
1
-
1
1
-
10
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
209218
Tsushida
An enzyme hydrolyzing L-theani ...
Camellia sinensis
Agric. Biol. Chem.
49
2913-2917
1985
2
-
-
-
-
2
16
-
-
1
-
2
-
1
-
-
1
-
-
1
1
1
10
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
-
-
-
2
-
16
-
-
-
1
-
2
-
-
-
1
-
1
1
1
10
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
209220
Soda
-
Metabolism of L-theanine, D-th ...
Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp., Pseudomonas sp. T-1
Agric. Biol. Chem.
30
541-546
1966
3
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
3
-
-
1
-
5
-
3
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
5
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
209221
Soda
-
Metabolism of L-theanine, D-th ...
Pseudomonas aeruginosa
Agric. Biol. Chem.
30
547-557
1966
-
-
-
-
-
-
5
2
-
1
-
1
-
1
-
-
1
-
-
-
1
-
7
-
1
-
2
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
1
-
1
-
-
-
1
-
-
1
-
7
-
1
-
2
-
2
1
1
-
-
-
-
-
-
-