We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 3.5.1.65 - theanine hydrolase for references in articles please use BRENDA:EC3.5.1.65
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
hydrolase, theanine, L-theanine hydrolase, L-theanine hydrolyzing enzyme, theanine ethylamidohydrolase, theanine hydrolytic enzyme, theanine hydrolyzing enzyme,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolase, theanine
-
-
-
-
L-theanine hydrolase
-
-
-
-
L-theanine hydrolyzing enzyme
-
-
-
-
theanine ethylamidohydrolase
-
-
-
-
theanine hydrolytic enzyme
-
-
-
-
theanine hydrolyzing enzyme
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
-
-
-
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of linear amides
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N5-ethyl-L-glutamine amidohydrolase
Also acts on other N-alkyl-L-glutamines.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glutamine + H2O
D-glutamate + NH3
-
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
-
?
N5-ethyl-D-glutamine + H2O
D-glutamate + ethylamine
-
D-theanine, probably by the same enzyme like L-theanine
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
N5-isobutyl-L-glutamine + H2O
L-glutamate + isobutylamine
-
gamma-glutamyl-isobutylamide, 136% of activity compared to L-theanine
-
-
?
N5-methyl-L-glutamine + H2O
L-glutamate + methylamine
-
gamma-glutamylmethylamide, 77% of activity compared to L-theanine
-
-
?
N5-n-butyl-L-glutamine + H2O
L-glutamate + n-butylamine
-
gamma-glutamyl-n-butylamide, 276% of activity compared to L-theanine
-
-
?
N5-n-pentyl-L-glutamine + H2O
L-glutamate + n-pentylamine
-
gamma-glutamyl-n-amylamide, 211% of activity compared to L-theanine
-
-
?
N5-n-propyl-L-glutamine + H2O
L-glutamate + n-propylamine
-
gamma-glutamyl-n-propylamide, 144% of activity compared to theanine
-
-
?
theanine + hydroxylamine
gamma-glutamylhydroxamic acid + ethylamine
-
transferase reaction
-
-
?
additional information
?
-
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
predominant amino acid of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
L-theanine, gamma-glutamylethylamide, principal component of taste of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
-
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
-
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
N-ethyl-gamma-L-glutamine
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
-
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
-
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
-
-
?
additional information
?
-
-
substrate specificity
-
-
-
additional information
?
-
-
substrate specificity
-
-
-
additional information
?
-
-
also acts on other N-alkyl-L-glutamines
-
-
-
additional information
?
-
-
also acts on other N-alkyl-L-glutamines
-
-
-
additional information
?
-
-
no activity with N-methylpropionamide and N-ethylpropionamide
-
-
-
additional information
?
-
-
no activity with N-methylpropionamide and N-ethylpropionamide
-
-
-
additional information
?
-
-
substrate specificity
-
-
-
additional information
?
-
-
no activity with L-asparagine, D-asparagine or acetamide
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
predominant amino acid of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
L-theanine, gamma-glutamylethylamide, principal component of taste of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
-
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
N-ethyl-gamma-L-glutamine
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
potassium ion and phosphate ion: no effect
additional information
-
no activation by phosphate ions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acetic acid
-
8 mM: 88% of activity compared to H2O
beta-Alanine
-
partial inhibition, 8 mM: 89% of activity compared to H2O
CuSO4
-
10 mM: strong inhibition
EDTA
-
10 mM: 30% inhibition
glycine
-
8 mM: 99% of activity compared to H2O
HgCl2
-
1 mM: strong inhibition
iodoacetate
-
2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol
L-asparagine
-
strong inhibition, 8 mM: 76% of activity compared to H2O
L-aspartic acid
-
strong inhibition, 8 mM: 53% of activity compared to H2O
L-Citric acid
-
8 mM: 95% of activity compared to H2O
L-Glutamic acid
-
strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O
L-glutamine
-
strong inhibition, 8 mM: 75% of activity compared to H2O
L-leucine
-
partial inhibition, 8 mM: 89% of activity compared to H2O
L-serine
-
partial inhibition, 8 mM: 95% of activity compared to H2O
Maleic acid
-
8 mM: 98% of activity compared to H2O
oxaloacetic acid
-
strong inhibition, 8 mM: 59% of activity compared to H2O
succinic acid
-
strong inhibition, 8 mM: 77% of activity compared to H2O
L-alanine
-
strong inhibition, 0.7 mM: 67%, 3.3 mM: 50%, 8 mM: 45%, 53.3 mM: 23% of activity compared to H2O
additional information
-
D-alanine, 8 mM, and D-malic acid, 8 mM: no effect
-
additional information
-
no inhibition by urea, p-chloromercuribenzoate, NaF or KF
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glutamate
-
activation and induction
L-glutamate
-
activation and induction
L-Theanine
-
activation and induction
additional information
-
D-malic acid, 8 mM, no effect
-
L-Malic acid
-
activation, 0.7 mM: 167%, 3.3 mM: 227%, 8 mM: 230%, 13.3 mM: 235% of activity compared to H2O
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
9.2
D-theanine
-
partial purified enzyme
5.8
L-Theanine
-
partial purified enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
additional information
-
-
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8
-
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.5 - 10.5
-
about half of maximum activity at pH 5.5 and 10.5
6.5 - 9
-
pH 6.5: about 22% of maximal activity, pH 9.0: about 95% of maximal activity
7.5
-
about half of maximum activity at pH 7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
37
-
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
T-1
-
-
brenda
T-1
-
-
brenda
cv. Yabukita; green tea; tea
-
-
brenda
cv. Yabukita; tea
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5 - 9.5
-
quite stable between
209221
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
55
-
60 min, stable at over a wide range of pH; above 55°C: rapid inactivation of enzyme
60
-
15 min, rapid inactivation of enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
theanine hydrolase activity increases slightly during first 10 h after plucking but thereafter decreased gradually
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4°C, 45% loss of activity within 1 day
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
partial, glutaminase could not be separated
partial, glutaminase could not be separated
-
partial, glutaminase could not be separated
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tsushida, T.; Takeo, T.
An enzyme hydrolyzing L-theanine in tea leaves
Agric. Biol. Chem.
49
2913-2917
1985
Camellia sinensis
-
brenda
Tsushida, T.
Metabolism of L-theanine in tea leaves
Jpn. Agric. Res. Q
21
42-46
1987
Camellia sinensis
-
brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products
Agric. Biol. Chem.
30
541-546
1966
Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp., Pseudomonas sp. T-1
-
brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part II. Purification and properties of the enzyme hydrolyzing L-theanine, D-theanine and the related compounds
Agric. Biol. Chem.
30
547-557
1966
Pseudomonas aeruginosa
-
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
3.5.1.65 theanine hydrolase
more
top
Information
