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Information on EC 3.5.1.65 - theanine hydrolase
for references in articles please use BRENDA:EC3.5.1.65
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EC Tree 3 Hydrolases
3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
3.5.1 In linear amides
3.5.1.65 theanine hydrolase
IUBMB Comments
Also acts on other N-alkyl-L-glutamines.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
showSynonyms
l-theanine hydrolase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CsGGT2
CsGGT4
gamma-glutamyl-transpeptidase
hydrolase, theanine
-
-
-
-
L-theanine hydrolase
L-theanine hydrolyzing enzyme
-
-
-
-
theanine ethylamidohydrolase
-
-
-
-
theanine hydrolytic enzyme
-
-
-
-
theanine hydrolyzing enzyme
-
-
-
-
L-theanine hydrolase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
Pseudomonas sp. T-1
-
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
N5-ethyl-L-glutamine amidohydrolase
Also acts on other N-alkyl-L-glutamines.
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CAS REGISTRY NUMBER
COMMENTARY
99533-51-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-glutamate + ethylamine
N5-ethyl-L-glutamine + H2O
-
Substrates: -
Products: -
Products: -
r
N5-ethyl-D-glutamine + H2O
D-glutamate + ethylamine
-
Substrates: D-theanine, probably by the same enzyme like L-theanine
Products: -
Products: -
?
N5-isobutyl-L-glutamine + H2O
L-glutamate + isobutylamine
-
Substrates: gamma-glutamyl-isobutylamide, 136% of activity compared to L-theanine
Products: -
Products: -
?
N5-methyl-L-glutamine + H2O
L-glutamate + methylamine
-
Substrates: gamma-glutamylmethylamide, 77% of activity compared to L-theanine
Products: -
Products: -
?
N5-n-butyl-L-glutamine + H2O
L-glutamate + n-butylamine
-
Substrates: gamma-glutamyl-n-butylamide, 276% of activity compared to L-theanine
Products: -
Products: -
?
N5-n-pentyl-L-glutamine + H2O
L-glutamate + n-pentylamine
-
Substrates: gamma-glutamyl-n-amylamide, 211% of activity compared to L-theanine
Products: -
Products: -
?
N5-n-propyl-L-glutamine + H2O
L-glutamate + n-propylamine
-
Substrates: gamma-glutamyl-n-propylamide, 144% of activity compared to theanine
Products: -
Products: -
?
theanine + hydroxylamine
gamma-glutamylhydroxamic acid + ethylamine
-
Substrates: transferase reaction
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: predominant amino acid of tea
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: L-theanine, gamma-glutamylethylamide, principal component of taste of tea
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
r
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
Camellia sinensis Huangkui
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: N-ethyl-gamma-L-glutamine
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
Pseudomonas sp. T-1
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: also acts on other N-alkyl-L-glutamines
Products: -
Products: -
?
additional information
?
-
-
Substrates: also acts on other N-alkyl-L-glutamines
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with N-methylpropionamide and N-ethylpropionamide
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with N-methylpropionamide and N-ethylpropionamide
Products: -
Products: -
?
additional information
?
-
Substrates: tobacco leaves that recombinantly overexpress the CsPDX2.1 gene can hydrolyze L-theanine to produce a large amount of ethylamine
Products: -
Products: -
-
additional information
?
-
Substrates: CsPDX2.1, specified as glutaminase (EC 3.5.1.2), is cloned and the recombinant protein catalyzes L-theanine hydrolysis into ethylamine and L-glutamate in vitro. The glutaminase activity is determined
Products: -
Products: -
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additional information
?
-
-
Substrates: CsGGT2 participates in theanine synthesis in vitro. Ligand analysis by HPLC-MS/MS and GC/MS analysis. The bifunctional CsGGT2 can synthesize and degrade theanine in vitro and in planta. The affinity of rCsGGT2 to glutamine and theanine is significantly higher than that to ethylamine hydrochloride. The enzymatic reaction of CsGGT2 is reversible, and preferentially responsible for hydrolase
Products: -
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additional information
?
-
-
Substrates: substrate specificity
Products: -
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?
additional information
?
-
-
Substrates: no activity with L-asparagine, D-asparagine or acetamide
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Substrates: tobacco leaves that recombinantly overexpress the CsPDX2.1 gene can hydrolyze L-theanine to produce a large amount of ethylamine
Products: -
Products: -
-
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: predominant amino acid of tea
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: L-theanine, gamma-glutamylethylamide, principal component of taste of tea
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
Camellia sinensis Huangkui
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: -
Products: -
Products: -
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
Substrates: N-ethyl-gamma-L-glutamine
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
NaCl
-
150 mM NaCl treatments result in an upward trend in the expression of CsGGT2 before 12 h and then decreases rapidly within 96 h. The content of theanine increases significantly under the 150 mM NaCl treatments. The content of theanine is increased by 3.55fold after 96 h of exposure to 150mM NaCl compared with the treatment for 6 h
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
iodoacetate
-
2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol
L-Glutamic acid
-
strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O
oxaloacetic acid
-
strong inhibition, 8 mM: 59% of activity compared to H2O
L-alanine
-
strong inhibition, 0.7 mM: 67%, 3.3 mM: 50%, 8 mM: 45%, 53.3 mM: 23% of activity compared to H2O
additional information
-
D-alanine, 8 mM, and D-malic acid, 8 mM: no effect
-
additional information
-
no inhibition by urea, p-chloromercuribenzoate, NaF or KF
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
L-Malic acid
-
activation, 0.7 mM: 167%, 3.3 mM: 227%, 8 mM: 230%, 13.3 mM: 235% of activity compared to H2O
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
Michaelis-Menten kinetics analysis. The equilibrium dissociation constant (KD) value of rCsGGT2 for theanine, glutamine and ethylamine hydrochloride are estimated to be 0.038, 0.024 and 0.112 M, respectively
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8
the enzyme activity is high at pH 6.0-6.5, but decreases rapidly when the pH exceeds pH 6.5 or above
6.5 - 9
-
pH 6.5: about 22% of maximal activity, pH 9.0: about 95% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35 - 45
recombinant enzyme, below 35°C the enzyme activity drops sharply
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene CsPDX2.1, specified as glutaminase, EC 3.5.1.2; green and yellow and white/albino varieties
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
quantitative RT-PCR enzyme expression analysis in different tissues, expression profiles of CsPDX2.1 gene in normal tea (green leaf) and albino mutants (white/yellow leaf), overview
brenda
additional information
-
expression patterns of CsGGT2 are significantly different in the aboveground and underground tissues of tea plants and regulated by light. CsGGT2 shows higher expression in strong light organs, than that of the stem and root, which are exposed to weak light and not exposed to light. CsGGT2 is specifically expressed in mature leaves with a low theanine content and is present at very low levels in the stems and roots, with a high theanine content. Overview
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
ethylamine subcellular distribution in tea leaves in mitochondria and peroxisomes
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
although other plants are unable to accumulate L-theanine itself, they contain some enzyme that has the ability to hydrolyze L-theanine. L-Theanine hydrolase is possibly ubiquitous in plants but not unique to tea plant. It is likely that PDX2 (glutaminase) fulfills the function of L-theanine hydrolase in tea plants, similar to glutamine synthetase, which could also be a L-theanine synthase in tea plants
malfunction
metabolism
physiological function
additional information
malfunction
CsPDX2.1 gene involves the weaker L-theanine hydrolysis ability in leaves of Huangyu mutant tea plants, three different yellow leave-typed cultivars
malfunction
Camellia sinensis Huangkui
-
silencing of CsGGT4 inhibits theanine synthetase activity
-
metabolism
-
gamma-glutamyl transpeptidase CsGGT4, as a homologous protein of the theanine hydrolase CsGGT2, exhibits a higher theanine (gamma-glutamyl-L-ethylamide) synthesis catalytic efficiency than CsGGT2. The theanine content correlates significantly with the expression of CsGGT2, CsGGT4, and the transcription factor CsMYB73 in tea shoots from different seasons. CsMYB73 directly interacts with the CsGGT2 and CsGGT4 promoters, serving as an activator of CsGGT2 and a suppressor of CsGGT4. MBP-tagged CsMYB73 displays specific binding to pro-CsGGT4. The degradation of theanine increases, concomitantly with the inhibition of theanine synthesis, resulting in a significant decline in the accumulation of theanine in tea shoots during the process of seasonal greening in cv. Huangkui leaves
metabolism
-
gamma-glutamyl transpeptidase CsGGT4, as a homologous protein of the theanine hydrolase CsGGT2, exhibits a higher theanine (gamma-glutamyl-L-ethylamide) synthesis catalytic efficiency than CsGGT2. The theanine content correlates significantly with the expression of CsGGT2, CsGGT4, and the transcription factor CsMYB73 in tea shoots from different seasons. CsMYB73 directly interacts with the CsGGT2 and CsGGT4 promoters, serving as an activator of CsGGT2 and a suppressor of CsGGT4. MBP-tagged CsMYB73 displays specific binding to pro-CsGGT2. The degradation of theanine increases, concomitantly with the inhibition of theanine synthesis, resulting in a significant decline in the accumulation of theanine in tea shoots during the process of seasonal greening in cv. Huangkui leaves
metabolism
Camellia sinensis Huangkui
-
gamma-glutamyl transpeptidase CsGGT4, as a homologous protein of the theanine hydrolase CsGGT2, exhibits a higher theanine (gamma-glutamyl-L-ethylamide) synthesis catalytic efficiency than CsGGT2. The theanine content correlates significantly with the expression of CsGGT2, CsGGT4, and the transcription factor CsMYB73 in tea shoots from different seasons. CsMYB73 directly interacts with the CsGGT2 and CsGGT4 promoters, serving as an activator of CsGGT2 and a suppressor of CsGGT4. MBP-tagged CsMYB73 displays specific binding to pro-CsGGT4. The degradation of theanine increases, concomitantly with the inhibition of theanine synthesis, resulting in a significant decline in the accumulation of theanine in tea shoots during the process of seasonal greening in cv. Huangkui leaves
-
physiological function
-
the unique proteinogenic amino acid, theanine (gamma-glutamyl-L-ethylamide) is dynamically regulated by environmental conditions and developmental cues involving the enzyme. Theanine acts as a form of nitrogen storage and transport and has vital physiological functions in plant growth. CsGGT2 and CsGGT4 have both synthesis and degradation functions in the production of theanine in vitro. CsGGT4 synthesizes and degrades theanine in vitro and in tea plants
physiological function
-
the unique proteinogenic amino acid, theanine (gamma-glutamyl-L-ethylamide) is dynamically regulated by environmental conditions and developmental cues involving the enzyme. Theanine acts as a form of nitrogen storage and transport and has vital physiological functions in plant growth. CsGGT2 and CsGGT4 have both synthesis and degradation functions in the production of theanine in vitro
physiological function
CsPDX2.1 is a bifunctional enzyme with L-glutamine hydrolase (EC 3.5.1.2) and L-theanine hydrolase functions. Lower hydrolysis of L-theanine (N5-ethyl-L-glutamine) into ethylamine and L-glutamate is a key factor that explains the higher content of L-theanine in albino tea with yellow or white leaves, compared with that of normal tea with green leaves. There are higher CsPDX2.1 transcript levels in leaf tissue and lower transcripts in the types of albino (yellow leaf) teas compared with green controls
physiological function
-
gamma-glutamyl-transpeptidase, CsGGT2, functions as light-activated theanine hydrolase in tea plant. CsGGT2 acts as bifunctional protein, synthesizing and degrading theanine in vitro and in planta. In the glutathione cycle, GGT catalyzes the transfer of gamma-glutamyl from glutathione and related gamma-glutamyl amino groups to amino acids and peptides to synthesize new compounds and can also specifically catalyze the cleavage of gamma-glutamate in glutathione and other gamma-glutamyl-containing compounds
physiological function
Camellia sinensis Huangkui
-
the unique proteinogenic amino acid, theanine (gamma-glutamyl-L-ethylamide) is dynamically regulated by environmental conditions and developmental cues involving the enzyme. Theanine acts as a form of nitrogen storage and transport and has vital physiological functions in plant growth. CsGGT2 and CsGGT4 have both synthesis and degradation functions in the production of theanine in vitro. CsGGT4 synthesizes and degrades theanine in vitro and in tea plants
-
physiological function
Camellia sinensis Huangkui
-
the unique proteinogenic amino acid, theanine (gamma-glutamyl-L-ethylamide) is dynamically regulated by environmental conditions and developmental cues involving the enzyme. Theanine acts as a form of nitrogen storage and transport and has vital physiological functions in plant growth. CsGGT2 and CsGGT4 have both synthesis and degradation functions in the production of theanine in vitro
-
additional information
-
homology modeling and molecular docking. Differential protein structures between CsGGT2 and CsGGT4 imply their different biological functions in tea plants
additional information
Camellia sinensis Huangkui
-
homology modeling and molecular docking. Differential protein structures between CsGGT2 and CsGGT4 imply their different biological functions in tea plants
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
silencing of the enzyme in leaves still attached to the plant, usage of the AsODN method for CsGGT2 gene silencing in tea cuttings
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
theanine hydrolase activity increases slightly during first 10 h after plucking but thereafter decreased gradually
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, glutaminase could not be separated
recombinant MBP-tagged CsGGT2 from Escherichia coli by amylose affinity chromatography
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene CsGGT2, transient recombinant overexpression in Nicotiana benthamiana leaves via trasnformation by Agrobacterium tumefaciens strain GV3101, quantitative real-time PCR enzyme expression analysis, recombinant expression of MBP-tagged CsGGT2 in Escherichia coli
-
gene CsPDX2.1, phylogenetic analysis and tree, recombinant expression in Escherichia coli, transient recombinant overexpression in Nicotiana benthamiana leaves via transformation by Agrobacterium tumefaciens strain GV3101. Tobacco leaves that overexpress the CsPDX2.1 gene can hydrolyze L-theanine to produce a large amount of ethylamine. Expression profiles of CsPDX2.1 gene in normal tea (green leaf) and albino mutants (white/yellow leaf), overview
recombinant expression of MBP-tagged CsGGT2 in Nicotiana benthamiana leaves, coexpression of transcription factor CsMYB73 and inducing regulatory effect analysis
-
recombinant expression of MBP-tagged CsGGT4 in Nicotiana benthamiana leaves, coexpression of transcription factor CsMYB73 and suppressing regulatory effect analysis
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
150 mM NaCl treatments result in an upward trend in the expression of CsGGT2 before 12 h and then decreases rapidly within 96 h
-
expression of CsGGT2 is upregulated under the light and is also negatively correlated with theanine accumulation in tea plants. Expression patterns of CsGGT2 are significantly different in the aboveground and underground tissues of tea plants and regulated by light. Light upregulates the expression of CsHY5, directly binding to the promoter of CsGGT2 and acting as an activator of CsGGT2, with a negative correlation with theanine accumulation. 150 mM NaCl treatments result in an upward trend in the expression of CsGGT2 before 12 h and then decreases rapidly within 96 h
-
light-regulated expression of CsGGT2 in plants
transcription factor CsMYB73 is confirmed to act as a nucleus-localized transcription factor (TF), that directly interacts with the CsGGT2 and CsGGT4 promoters, serving as an activator of CsGGT2 and a suppressor of CsGGT4. This leads to a negative association with theanine accumulation in tea shoots. Furthermore, the continuous increase in CsMYB73 produces a significant increase in CsGGT2 expression and inhibits CsGGT4 expression
transcription factor CsMYB73 is confirmed to act as a nucleus-localized transcription factor (TF), that directly interacts with the CsGGT2 and CsGGT4 promoters, serving as an activator of CsGGT2 and a suppressor of CsGGT4. This leads to a negative association with theanine accumulation in tea shoots. Furthermore, the continuous increase in CsMYB73 produces a significant increase in CsGGT2 expression and inhibits CsGGT4 expression
-
transcription factor CsMYB73 is confirmed to act as a nucleus-localized transcription factor (TF), that directly interacts with the CsGGT2 and CsGGT4 promoters, serving as an activator of CsGGT2 and a suppressor of CsGGT4. This leads to a negative association with theanine accumulation in tea shoots. Furthermore, the continuous increase in CsMYB73 produces a significant increase in CsGGT2 expression and inhibits CsGGT4 expression
Camellia sinensis Huangkui
-
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tsushida, T.; Takeo, T.
An enzyme hydrolyzing L-theanine in tea leaves
Agric. Biol. Chem.
49
2913-2917
1985
Camellia sinensis
-
brenda
Tsushida, T.
Metabolism of L-theanine in tea leaves
Jpn. Agric. Res. Q
21
42-46
1987
Camellia sinensis
-
brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products
Agric. Biol. Chem.
30
541-546
1966
Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp., Pseudomonas sp. T-1
-
brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part II. Purification and properties of the enzyme hydrolyzing L-theanine, D-theanine and the related compounds
Agric. Biol. Chem.
30
547-557
1966
Pseudomonas aeruginosa
-
brenda
Chang, M.; Sun, Y.; Fang, K.; Fu, M.; Ma, J.; Gao, Y.; Chen, Q.; Liu, L.; Zhang, Z.; Wan, X.; Sun, J.
CsMYB73 negatively regulates theanine accumulation mediated by CsGGT2 and CsGGT4 in tea shoots (Camellia sinensis)
Hortic. Res.
11
uhae012
2024
Camellia sinensis, Camellia sinensis Huangkui
brenda
Fu, X.; Cheng, S.; Liao, Y.; Xu, X.; Wang, X.; Hao, X.; Xu, P.; Dong, F.; Yang, Z.
Characterization of L-theanine hydrolase in vitro and subcellular distribution of its specific product ethylamine in tea (Camellia sinensis)
J. Agric. Food Chem.
68
10842-10851
2020
Camellia sinensis (A0A7G9ULP3)
brenda
Chang, M.; Ma, J.; Sun, Y.; Tian, L.; Liu, L.; Chen, Q.; Zhang, Z.; Wan, X.; Sun, J.
gamma-Glutamyl-transpeptidase CsGGT2 functions as light-activated theanine hydrolase in tea plant (Camellia sinensis L.)
Plant Cell Environ.
46
1596-1609
2023
Camellia sinensis
brenda
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Release 2026.1 (March 2026)
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