BRENDA home
History
Information on EC 3.5.1.65 - theanine hydrolase
for references in articles please use BRENDA:EC3.5.1.65Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.5.1.65 theanine hydrolaseIUBMB Comments
Also acts on other N-alkyl-L-glutamines.
Specify your search results
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
hydrolase, theanine, L-theanine hydrolase, L-theanine hydrolyzing enzyme, theanine ethylamidohydrolase, theanine hydrolytic enzyme, theanine hydrolyzing enzyme, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
hydrolase, theanine
-
-
-
-
L-theanine hydrolase
-
-
-
-
L-theanine hydrolyzing enzyme
-
-
-
-
theanine ethylamidohydrolase
-
-
-
-
theanine hydrolytic enzyme
-
-
-
-
theanine hydrolyzing enzyme
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
Pseudomonas sp. T-1
-
-
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
N5-ethyl-L-glutamine amidohydrolase
Also acts on other N-alkyl-L-glutamines.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
99533-51-4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N5-ethyl-D-glutamine + H2O
D-glutamate + ethylamine
-
D-theanine, probably by the same enzyme like L-theanine
-
?
N5-isobutyl-L-glutamine + H2O
L-glutamate + isobutylamine
-
gamma-glutamyl-isobutylamide, 136% of activity compared to L-theanine
-
-
?
N5-methyl-L-glutamine + H2O
L-glutamate + methylamine
-
gamma-glutamylmethylamide, 77% of activity compared to L-theanine
-
-
?
N5-n-butyl-L-glutamine + H2O
L-glutamate + n-butylamine
-
gamma-glutamyl-n-butylamide, 276% of activity compared to L-theanine
-
-
?
N5-n-pentyl-L-glutamine + H2O
L-glutamate + n-pentylamine
-
gamma-glutamyl-n-amylamide, 211% of activity compared to L-theanine
-
-
?
N5-n-propyl-L-glutamine + H2O
L-glutamate + n-propylamine
-
gamma-glutamyl-n-propylamide, 144% of activity compared to theanine
-
-
?
theanine + hydroxylamine
gamma-glutamylhydroxamic acid + ethylamine
-
transferase reaction
-
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
predominant amino acid of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
L-theanine, gamma-glutamylethylamide, principal component of taste of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
N-ethyl-gamma-L-glutamine
-
?
additional information
?
-
-
no activity with N-methylpropionamide and N-ethylpropionamide
-
-
?
additional information
?
-
-
no activity with N-methylpropionamide and N-ethylpropionamide
-
-
?
additional information
?
-
-
no activity with L-asparagine, D-asparagine or acetamide
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
predominant amino acid of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
L-theanine, gamma-glutamylethylamide, principal component of taste of tea
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
-
N-ethyl-gamma-L-glutamine
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
iodoacetate
-
2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol
L-Glutamic acid
-
strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O
oxaloacetic acid
-
strong inhibition, 8 mM: 59% of activity compared to H2O
L-alanine
-
strong inhibition, 0.7 mM: 67%, 3.3 mM: 50%, 8 mM: 45%, 53.3 mM: 23% of activity compared to H2O
additional information
-
D-alanine, 8 mM, and D-malic acid, 8 mM: no effect
-
additional information
-
no inhibition by urea, p-chloromercuribenzoate, NaF or KF
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
L-Malic acid
-
activation, 0.7 mM: 167%, 3.3 mM: 227%, 8 mM: 230%, 13.3 mM: 235% of activity compared to H2O
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 9
-
pH 6.5: about 22% of maximal activity, pH 9.0: about 95% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
theanine hydrolase activity increases slightly during first 10 h after plucking but thereafter decreased gradually
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, glutaminase could not be separated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tsushida, T.; Takeo, T.
An enzyme hydrolyzing L-theanine in tea leaves
Agric. Biol. Chem.
49
2913-2917
1985
Camellia sinensis
-
brenda
Tsushida, T.
Metabolism of L-theanine in tea leaves
Jpn. Agric. Res. Q
21
42-46
1987
Camellia sinensis
-
brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products
Agric. Biol. Chem.
30
541-546
1966
Pseudomonas sp., Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp. T-1
-
brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part II. Purification and properties of the enzyme hydrolyzing L-theanine, D-theanine and the related compounds
Agric. Biol. Chem.
30
547-557
1966
Pseudomonas aeruginosa
-
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 3.5.1.65)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
About BRENDA
Help
Download
Contribution
member of
curated at
curated at
Server: brenda1 Ver: 69ad221-main (2022-06-24 14:26:09 +0200)