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Information on EC 3.5.1.65 - theanine hydrolase for references in articles please use BRENDA:EC3.5.1.65
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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
hydrolase, theanine, L-theanine hydrolase, L-theanine hydrolyzing enzyme, theanine ethylamidohydrolase, theanine hydrolytic enzyme, theanine hydrolyzing enzyme,
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hydrolase, theanine
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L-theanine hydrolase
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L-theanine hydrolyzing enzyme
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theanine ethylamidohydrolase
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theanine hydrolytic enzyme
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theanine hydrolyzing enzyme
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N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
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N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
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N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
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N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
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N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
stoichiometry of reaction
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hydrolysis of linear amides
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N5-ethyl-L-glutamine amidohydrolase
Also acts on other N-alkyl-L-glutamines.
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D-glutamine + H2O
D-glutamate + NH3
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?
L-glutamine + H2O
L-glutamate + NH3
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?
N5-ethyl-D-glutamine + H2O
D-glutamate + ethylamine
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D-theanine, probably by the same enzyme like L-theanine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
N5-isobutyl-L-glutamine + H2O
L-glutamate + isobutylamine
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gamma-glutamyl-isobutylamide, 136% of activity compared to L-theanine
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?
N5-methyl-L-glutamine + H2O
L-glutamate + methylamine
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gamma-glutamylmethylamide, 77% of activity compared to L-theanine
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?
N5-n-butyl-L-glutamine + H2O
L-glutamate + n-butylamine
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gamma-glutamyl-n-butylamide, 276% of activity compared to L-theanine
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?
N5-n-pentyl-L-glutamine + H2O
L-glutamate + n-pentylamine
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gamma-glutamyl-n-amylamide, 211% of activity compared to L-theanine
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?
N5-n-propyl-L-glutamine + H2O
L-glutamate + n-propylamine
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gamma-glutamyl-n-propylamide, 144% of activity compared to theanine
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?
theanine + hydroxylamine
gamma-glutamylhydroxamic acid + ethylamine
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transferase reaction
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?
additional information
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N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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predominant amino acid of tea
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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L-theanine, gamma-glutamylethylamide, principal component of taste of tea
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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N-ethyl-gamma-L-glutamine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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additional information
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substrate specificity
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additional information
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substrate specificity
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additional information
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also acts on other N-alkyl-L-glutamines
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additional information
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also acts on other N-alkyl-L-glutamines
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additional information
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no activity with N-methylpropionamide and N-ethylpropionamide
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additional information
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no activity with N-methylpropionamide and N-ethylpropionamide
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additional information
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substrate specificity
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additional information
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no activity with L-asparagine, D-asparagine or acetamide
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N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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predominant amino acid of tea
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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L-theanine, gamma-glutamylethylamide, principal component of taste of tea
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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part in metabolism of theanine in tea leaves: theanine is involved in the biosynthesis of other compound in tea plants, theanine also appears to be involved in storage or transport of nitrogen in non-toxic form in tea plants
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
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N-ethyl-gamma-L-glutamine
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?
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additional information
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potassium ion and phosphate ion: no effect
additional information
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no activation by phosphate ions
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acetic acid
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8 mM: 88% of activity compared to H2O
beta-Alanine
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partial inhibition, 8 mM: 89% of activity compared to H2O
CuSO4
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10 mM: strong inhibition
EDTA
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10 mM: 30% inhibition
glycine
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8 mM: 99% of activity compared to H2O
HgCl2
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1 mM: strong inhibition
iodoacetate
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2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol
L-asparagine
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strong inhibition, 8 mM: 76% of activity compared to H2O
L-aspartic acid
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strong inhibition, 8 mM: 53% of activity compared to H2O
L-Citric acid
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8 mM: 95% of activity compared to H2O
L-Glutamic acid
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strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O
L-glutamine
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strong inhibition, 8 mM: 75% of activity compared to H2O
L-leucine
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partial inhibition, 8 mM: 89% of activity compared to H2O
L-serine
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partial inhibition, 8 mM: 95% of activity compared to H2O
Maleic acid
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8 mM: 98% of activity compared to H2O
oxaloacetic acid
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strong inhibition, 8 mM: 59% of activity compared to H2O
succinic acid
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strong inhibition, 8 mM: 77% of activity compared to H2O
L-alanine
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strong inhibition, 0.7 mM: 67%, 3.3 mM: 50%, 8 mM: 45%, 53.3 mM: 23% of activity compared to H2O
additional information
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D-alanine, 8 mM, and D-malic acid, 8 mM: no effect
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additional information
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no inhibition by urea, p-chloromercuribenzoate, NaF or KF
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D-glutamate
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activation and induction
L-glutamate
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activation and induction
L-Theanine
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activation and induction
additional information
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D-malic acid, 8 mM, no effect
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L-Malic acid
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activation, 0.7 mM: 167%, 3.3 mM: 227%, 8 mM: 230%, 13.3 mM: 235% of activity compared to H2O
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9.2
D-theanine
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partial purified enzyme
5.8
L-Theanine
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partial purified enzyme
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additional information
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additional information
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additional information
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8
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assay at
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5.5 - 10.5
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about half of maximum activity at pH 5.5 and 10.5
6.5 - 9
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pH 6.5: about 22% of maximal activity, pH 9.0: about 95% of maximal activity
7.5
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about half of maximum activity at pH 7.5
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37
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assay at
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brenda
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brenda
T-1
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brenda
T-1
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brenda
cv. Yabukita; green tea; tea
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brenda
cv. Yabukita; tea
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brenda
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brenda
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5 - 9.5
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quite stable between
209221
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55
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60 min, stable at over a wide range of pH; above 55°C: rapid inactivation of enzyme
60
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15 min, rapid inactivation of enzyme
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theanine hydrolase activity increases slightly during first 10 h after plucking but thereafter decreased gradually
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4°C, 45% loss of activity within 1 day
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partial, glutaminase could not be separated
partial, glutaminase could not be separated
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partial, glutaminase could not be separated
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Tsushida, T.; Takeo, T.
An enzyme hydrolyzing L-theanine in tea leaves
Agric. Biol. Chem.
49
2913-2917
1985
Camellia sinensis
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brenda
Tsushida, T.
Metabolism of L-theanine in tea leaves
Jpn. Agric. Res. Q
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42-46
1987
Camellia sinensis
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brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part I. Bacterial and enzymatic hydrolysis of L- and D-isomers of theanine and identification of products
Agric. Biol. Chem.
30
541-546
1966
Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas sp., Pseudomonas sp. T-1
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brenda
Soda, K.; Uchiyama, K.; Ogata, K.
Metabolism of L-theanine, D-theanine and the related compounds in bacteria. Part II. Purification and properties of the enzyme hydrolyzing L-theanine, D-theanine and the related compounds
Agric. Biol. Chem.
30
547-557
1966
Pseudomonas aeruginosa
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brenda
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