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Literature summary for 3.5.1.52 extracted from
- Unique peptide:N-glycanase of Caenorhabditis elegans has activity of protein disulphide reductase as well as of deglycosylation (2007), J. Biochem., 142, 175-181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and CHO cells | Caenorhabditis elegans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, 19% inhibition | Caenorhabditis elegans |  |
| carbobenzyloxy-Val-Ala-Asp-alpha-fluoromethylketone | - |
Caenorhabditis elegans | |
| Co2+ | 2 mM, almost complete inhibition | Caenorhabditis elegans | |
| Cu2+ | 2 mM, almost complete inhibition | Caenorhabditis elegans | |
| Fe2+ | 2 mM, 90% inhibition | Caenorhabditis elegans | |
| Mg2+ | 2 mM, 14% inhibition | Caenorhabditis elegans | |
| Mn2+ | 2 mM, 56% inhibition | Caenorhabditis elegans | |
| Zn2+ | 2 mM, almost complete inhibition | Caenorhabditis elegans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Caenorhabditis elegans | 5829 | - |
| endoplasmic reticulum | - |
Caenorhabditis elegans | 5783 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, SDS-PAGE | Caenorhabditis elegans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | Q9TW67 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caenorhabditis elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNAse + H2O | removes high mannose-type N-glycan from denatured RNase B, but not from native RNase B | Caenorhabditis elegans | ? | - |
? | |
| sialylglycopeptide + H2O | asialo-, agalactosyl-, trimannosyl- and monomanosyl-sialylglycopeptides are hydrolyzed | Caenorhabditis elegans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, SDS-PAGE | Caenorhabditis elegans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptide:N-glycanase | - |
Caenorhabditis elegans |
| PNGase | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Caenorhabditis elegans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Caenorhabditis elegans |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
- |
Caenorhabditis elegans | carbobenzyloxy-Val-Ala-Asp-alpha-fluoromethylketone | |
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Release 2023.1 (January 2023)
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