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Literature summary for 3.5.1.52 extracted from

  • Seko, A.; Kitajima, K.; Iwamatsu, T.; Inoue, Y.; Inoue, S.
    Identification of two dicrete peptide:N-glycanases in Oryzias latipes during embryogenesis (1999), Glycobiology, 9, 887-895.
    View publication on PubMed
Search for more literature for 3.5.1.52

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 nearly complete inhibition of the acid PNGase M by 5 mM, 10-20% remaining activity at neutral PNGase M by 5 mM Oryzias latipes
FeCl3 nearly complete inhibition of the acid PNGase M by 5 mM, 10-20% remaining activity at neutral PNGase M by 5 mM Oryzias latipes
L-hyosphorin derived nonapeptide 1 mM causes 92% inbition of acid PNGase M Oryzias latipes
additional information no effect: 1 mM peptide free N-linked glycan, 1 mM de-N-glycosylated peptide, 5 mM phenylmethylsulfonyl fluoride at neutral PNGase M; no effect: 5 mM monoiodoacetic acid, 0.5 mM p-chloromercurobenzoic acid, 5 mM ZnCl2 at acid PNGase M Oryzias latipes
p-chloromercuribenzoic acid 0.5 mM causes 89% inhibition of neutral PNGase M Oryzias latipes
ZnCl2 10-20% remaining activity at neutral PNGase M by 5 mM Oryzias latipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 estimation for acid PNGase M by gel filtration Oryzias latipes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Oryzias latipes glycophosphoprotein MU-1 + H2O Oryzias latipes acid PNGase M from blastoderm stage 11, yolk-absorptive stage ?
-
 ?
Oryzias latipes glycophosphoprotein MU-2 + H2O Oryzias latipes acid PNGase M from blastoderm stage 11, yolk-absorptive stage ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryzias latipes
-
 medaka fish
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryzias latipes

Source Tissue

Source Tissue Comment Organism Textmining
blastoderm
-
 Oryzias latipes
-
embryo
-
 Oryzias latipes
-
ovary
-
 Oryzias latipes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bence-jones wh lambda glycopeptide + H2O acid PNGase M from blastoderm stage 11 Oryzias latipes ?
-
 ?
fetuin glycopeptide II + H2O acid PNGase M from blastoderm stage 11 Oryzias latipes ?
-
 ?
L-hyosophorin + H2O neutral and acid PNGase M Oryzias latipes ?
-
 ?
Oryzias latipes glycophosphoprotein MU-1 + H2O acid PNGase M from blastoderm stage 11, yolk-absorptive stage Oryzias latipes ?
-
 ?
Oryzias latipes glycophosphoprotein MU-2 + H2O acid PNGase M from blastoderm stage 11, yolk-absorptive stage Oryzias latipes ?
-
 ?
ovalbumin glycopeptide + H2O Glu-Glu-Lys-Tyr-Asn(oligosaccharide)-Leu-Thr-Ser-Val Oryzias latipes aspartic acid + NH3 + N-acetylglucosamine
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4 acid PNGase M, whole embryo, stage 18-28 Oryzias latipes
7.5 8.5 neutral PNGase M, middle blastula blastoderm, stage 7-13 Oryzias latipes