Application | Comment | Organism |
---|---|---|
industry | the immobilized, cross-linked enzyme aggregate proves useful as a substitute for soluble amidase as a biocatalyst in the pharmaceutical and chemical industries | Rhodococcus erythropolis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an immobilized, cross-linked enzyme aggregate by mixing of the recombinant, crude, soluble enzyme with bovine serum albumin, and ammonium sulfate precipitation, followed by cross-linking of the resultant precipitant with glutaraldehyde. The enzyme aggregate shows hydrolytic activity toward a variety of amide substrates, and increased reaction stability | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloro-3-hydroxybutyramide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme | Rhodococcus erythropolis | 4-chloro-3-hydroxybutyrate + NH3 | - |
? | |
acetamide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, low activity | Rhodococcus erythropolis | acetate + NH3 | - |
? | |
acrylamide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme | Rhodococcus erythropolis | acrylate + NH3 | - |
? | |
benzamide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme | Rhodococcus erythropolis | benzoate + NH3 | - |
? | |
butyramide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme | Rhodococcus erythropolis | butyrate + NH3 | - |
? | |
isobutyramide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, preferred substrate | Rhodococcus erythropolis | isobutyrate + NH3 | - |
? | |
propionamide + H2O | substrate of the soluble enzyme and of the immobilized, cross-linked enzyme | Rhodococcus erythropolis | propionate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
immobilized, cross-linked enzyme aggregate | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
immobilized, cross-linked enzyme aggregate | Rhodococcus erythropolis |