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Literature summary for 3.5.1.4 extracted from

  • Hildmann, C.; Ninkovic, M.; Dietrich, R.; Wegener, D.; Riester, D.; Zimmermann, T.; Birch, O.M.; Bernegger, C.; Loidl, P.; Schwienhorst, A.
    A new amidohydrolase from Bordetella or Alcaligenes strain FB188 with similarities to histone deacetylases (2004), J. Bacteriol., 186, 2328-2339.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
suberoylanilide hydroxamic acid strong inhibition Alcaligenes sp.
tricostatin A strong inhibition Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 acetate-prelabeled chicken histones
-
 Alcaligenes sp.
0.127
-
 Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) pH 7.5, 37°C Alcaligenes sp.
4
-
 cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide pH 7.5, 37°C Alcaligenes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation above 100 mM Alcaligenes sp.
Co2+ increases 1.3fold the activity at 1 mM, inhibition at higher concentration Alcaligenes sp.
Mg2+ activation above 100 mM Alcaligenes sp.
Mn2+ activation above 100 mM Alcaligenes sp.
Ni2+ increases 1.1fold the activity at 1 mM, inhibition at higher concentration Alcaligenes sp.
Zn2+ required for activity Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39420
-
 from amino acid sequence Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. Q70I53 strain FB188, or Bordetella sp.
-
Alcaligenes sp. DSM 11172 Q70I53 strain FB188, or Bordetella sp.
-

Purification (Commentary)

Purification (Comment) Organism
several chromatographic steps, including Q-Sepharose and Sephacryl S-100 Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
 acetylspermidine, pH 7.5 Alcaligenes sp.
0.008
-
 acetylcadaverine, pH 7.5 Alcaligenes sp.
0.011
-
 acetylputrescine, pH 7.5 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate-prelabeled chicken histones + H2O
-
 Alcaligenes sp. deacetylated chicken histones + acetate
-
 ?
acetate-prelabeled chicken histones + H2O
-
 Alcaligenes sp. DSM 11172 deacetylated chicken histones + acetate
-
 ?
acetylcadaverine + H2O
-
 Alcaligenes sp. acetate + cadaverine
-
 ?
acetylcadaverine + H2O
-
 Alcaligenes sp. DSM 11172 acetate + cadaverine
-
 ?
acetylputrescine + H2O
-
 Alcaligenes sp. acetate + putrescine
-
 ?
acetylputrescine + H2O
-
 Alcaligenes sp. DSM 11172 acetate + putrescine
-
 ?
acetylspermidine + H2O
-
 Alcaligenes sp. acetate + spermidine
-
 ?
acetylspermidine + H2O
-
 Alcaligenes sp. DSM 11172 acetate + spermidine
-
 ?
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
-
 Alcaligenes sp. Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
-
 ?
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
-
 Alcaligenes sp. DSM 11172 Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
-
 ?
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide + H2O used as enzymatic assay Alcaligenes sp. cis-(1S,4R)-(4-(hydroxymethyl)cyclopent-2-enyl)amine + acetate
-
 ?

Subunits

Subunits Comment Organism
monomer 1* 35000, SDS-PAGE Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Alcaligenes sp.