Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Delftia acidovorans AE1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
lysis of crude extract, addition of streptomycin, ammonium sulfate fractionation, desalting by Sephadex-gel filtration, DEAE-Sepharose chromatography, hydroxyapatite column, yield 72% | Delftia acidovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
125 | - |
substrate acetanilide, after disc electrophoresis of purified enzyme | Delftia acidovorans |
131 | - |
substrate acetanilide, after purification | Delftia acidovorans |
Storage Stability | Organism |
---|---|
glycerol 2%, v/v, stabilizes the enzyme, after storing three months at -18°C, 77% of the activity of purified enzyme recovered | Delftia acidovorans |