BRENDA - Enzyme Database
show all sequences of 3.5.1.32

Hippurathydrolase, ein neues Enzym aus Mikroorganismen, Darstellung einer gereinigten Enzympräparation und Untersuchungen zur Substratspezifität

Röhr, M.; Monatsh. Chem. 99, 2255-2277 (1968)
No PubMed abstract available

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Fusarium incarnatum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Fusarium incarnatum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
induction of enzyme synthesis by hippurate-containing media
Fusarium incarnatum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
benzyloxycarbonyl-glycyl-L-phenylalanine + H2O
-
172035
Fusarium incarnatum
benzoic acid + ?
-
172035
Fusarium incarnatum
?
glycyl-L-leucine + H2O
-
172035
Fusarium incarnatum
glycine + L-leucine
-
172035
Fusarium incarnatum
?
glycylglycylglycine + H2O
-
172035
Fusarium incarnatum
glycine + glycylglycine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-alanine + H2O
-
172035
Fusarium incarnatum
acetic acid + DL-alanine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-asparagine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + DL-asparagine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-methionine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + DL-methionine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-phenylalanine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + DL-phenylalanine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-tryptophan + H2O
-
172035
Fusarium incarnatum
acetic acid + DL-tryptophan
-
172035
Fusarium incarnatum
?
N-acetyl-DL-valine + H2O
very low activity
172035
Fusarium incarnatum
acetic acid + DL-valine
-
172035
Fusarium incarnatum
?
N-acetyl-glycine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + glycine
-
172035
Fusarium incarnatum
?
N-acetyl-L-cysteine + H2O
-
172035
Fusarium incarnatum
acetic acid + L-cysteine
-
172035
Fusarium incarnatum
?
N-acetyl-L-glutamine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + L-glutamine
-
172035
Fusarium incarnatum
?
N-benzoyl-(S-benzoyl)-DL-cysteine + H2O
low activity
172035
Fusarium incarnatum
benzoic acid + S-benzoyl-DL-cysteine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-alanine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-alanine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-alloisoleucine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-alloisoleucine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-arginine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-arginine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-asparagine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-asparagine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-glutamine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-glutamine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-histidine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-histidine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-leucine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-leucine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-methionine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-methionine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-phenylalanine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-phenylalanine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-serine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-serine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-threonine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-threonine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-tryptophan + H2O
low activity
172035
Fusarium incarnatum
benzoic acid + DL-tryptophan
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-tyrosine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-tyrosine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-valine + H2O
low activity
172035
Fusarium incarnatum
benzoic acid + DL-valine
-
172035
Fusarium incarnatum
?
N-benzoylglycine + H2O
best substrate
172035
Fusarium incarnatum
benzoic acid + glycine
-
172035
Fusarium incarnatum
?
N-benzoylglycine + H2O
hippurate
172035
Fusarium incarnatum
benzoic acid + glycine
-
172035
Fusarium incarnatum
?
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
extremely labile below and above
Fusarium incarnatum
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Fusarium incarnatum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
induction of enzyme synthesis by hippurate-containing media
Fusarium incarnatum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
benzyloxycarbonyl-glycyl-L-phenylalanine + H2O
-
172035
Fusarium incarnatum
benzoic acid + ?
-
172035
Fusarium incarnatum
?
glycyl-L-leucine + H2O
-
172035
Fusarium incarnatum
glycine + L-leucine
-
172035
Fusarium incarnatum
?
glycylglycylglycine + H2O
-
172035
Fusarium incarnatum
glycine + glycylglycine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-alanine + H2O
-
172035
Fusarium incarnatum
acetic acid + DL-alanine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-asparagine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + DL-asparagine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-methionine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + DL-methionine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-phenylalanine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + DL-phenylalanine
-
172035
Fusarium incarnatum
?
N-acetyl-DL-tryptophan + H2O
-
172035
Fusarium incarnatum
acetic acid + DL-tryptophan
-
172035
Fusarium incarnatum
?
N-acetyl-DL-valine + H2O
very low activity
172035
Fusarium incarnatum
acetic acid + DL-valine
-
172035
Fusarium incarnatum
?
N-acetyl-glycine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + glycine
-
172035
Fusarium incarnatum
?
N-acetyl-L-cysteine + H2O
-
172035
Fusarium incarnatum
acetic acid + L-cysteine
-
172035
Fusarium incarnatum
?
N-acetyl-L-glutamine + H2O
low activity
172035
Fusarium incarnatum
acetic acid + L-glutamine
-
172035
Fusarium incarnatum
?
N-benzoyl-(S-benzoyl)-DL-cysteine + H2O
low activity
172035
Fusarium incarnatum
benzoic acid + S-benzoyl-DL-cysteine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-alanine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-alanine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-alloisoleucine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-alloisoleucine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-arginine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-arginine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-asparagine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-asparagine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-glutamine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-glutamine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-histidine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-histidine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-leucine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-leucine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-methionine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-methionine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-phenylalanine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-phenylalanine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-serine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-serine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-threonine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-threonine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-tryptophan + H2O
low activity
172035
Fusarium incarnatum
benzoic acid + DL-tryptophan
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-tyrosine + H2O
-
172035
Fusarium incarnatum
benzoic acid + DL-tyrosine
-
172035
Fusarium incarnatum
?
N-benzoyl-DL-valine + H2O
low activity
172035
Fusarium incarnatum
benzoic acid + DL-valine
-
172035
Fusarium incarnatum
?
N-benzoylglycine + H2O
best substrate
172035
Fusarium incarnatum
benzoic acid + glycine
-
172035
Fusarium incarnatum
?
N-benzoylglycine + H2O
hippurate
172035
Fusarium incarnatum
benzoic acid + glycine
-
172035
Fusarium incarnatum
?
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
extremely labile below and above
Fusarium incarnatum
Other publictions for EC 3.5.1.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670749
Steele
Enzymatic activity of Campylob ...
Campylobacter jejuni
Protein Eng. Des. Sel.
19
17-25
2006
1
-
1
-
6
-
11
11
-
4
2
1
-
9
-
-
1
1
-
-
1
-
11
2
1
1
-
-
17
1
-
-
-
-
-
-
1
-
1
-
-
6
-
-
11
-
11
-
4
2
1
-
-
-
1
-
-
1
-
11
2
1
-
-
17
1
-
-
-
-
-
-
-
-
-
172032
Ito
Enzymic determination of itoic ...
Pseudomonas putida
Appl. Environ. Microbiol.
59
2343-2345
1993
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
172033
Miyagawa
-
Crystallization and properties ...
Pseudomonas putida
Agric. Biol. Chem.
49
2881-2886
1985
-
-
-
1
-
-
13
11
-
-
2
-
-
1
-
-
1
-
-
1
1
-
17
1
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
13
-
11
-
-
2
-
-
-
-
1
-
1
1
-
17
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
172034
Ottow
Detection of hippurate hydrola ...
Geobacillus stearothermophilus, Paenibacillus alvei, Brevibacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus coagulans, Metabacillus fastidiosus, Cytobacillus firmus, Priestia megaterium, Sporosarcina globispora, Psychrobacillus insolitus, Sporolactobacillus laevolacticus, Brevibacillus laterosporus, Bacillus licheniformis, Paenibacillus macerans, Peribacillus simplex, Bacillus pumilus, Virgibacillus pantothenticus, Sporosarcina pasteurii, Paenibacillus polymyxa, Sporosarcina psychrophila, Paenibacillus larvae subsp. pulvifaciens, Bacillus racemilacticus, Lysinibacillus sphaericus, no activity in Bacillus lentus, no activity in Bacillus macquariensis
J. Appl. Bacteriol.
37
15-30
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
28
-
-
-
-
-
24
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
24
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
172035
Röhr
-
Hippurathydrolase, ein neues E ...
Fusarium incarnatum
Monatsh. Chem.
99
2255-2277
1968
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
29
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
29
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
172036
Röhr
-
Hippurathydrolase, ein neues E ...
Fusarium incarnatum
Monatsh. Chem.
99
2278-2290
1968
-
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
1
-
-
1
1
1
1
-
-
1
-
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
2
-
1
1
1
-
-
-
-
-
-
-