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4-acetyl-N-benzoyl-L-alanine + H2O
4-acetylbenzoic acid + L-alanine
-
low activity
-
?
4-acetyl-N-benzoylglycine + H2O
4-acetylbenzoic acid + glycine
-
-
-
?
4-aminobenzoyl-glutamate + H2O
4-aminobenzoate + glutamate
-
-
-
-
?
4-chloro-N-benzoyl-L-alanine + H2O
4-chlorobenzoic acid + L-alanine
-
-
-
?
4-chloro-N-benzoylglycine + H2O
4-chlorobenzoic acid + glycine
-
-
-
?
4-hydroxy-N-benzoyl-L-alanine
4-hydroxybenzoic acid + L-alanine
-
low activity
-
?
4-hydroxy-N-benzoylglycine + H2O
4-hydroxybenzoic acid + glycine
-
-
-
?
4-hydroxy-N-benzoylglycine-L-histidyl-L-leucine + H2O
4-hydroxybenzoic acid + L-histidyl-L-leucine
-
-
-
?
4-nitro-N-benzoyl-L-alanine + H2O
4-nitrobenzoic acid + L-alanine
-
-
-
?
4-nitro-N-benzoylglycine + H2O
4-nitrobenzoic acid + glycine
-
best substrate
-
?
benzyloxycarbonyl-glycyl-L-phenylalanine + H2O
benzoic acid + ?
-
-
-
?
glycyl-L-leucine + H2O
glycine + L-leucine
-
-
-
?
glycylglycylglycine + H2O
glycine + glycylglycine
-
-
-
?
hippurate + H2O
benzoate + glycine
itoic acid + H2O
2,3-dihydroxybenzoic acid + glycine
-
low specificity
-
?
N-(4-nitrobenzoyl)-Gly-Gly-Gly + H2O
4-nitrobenzoic acid + Gly-Gly-Gly
-
-
-
?
N-acetyl-DL-alanine + H2O
acetic acid + DL-alanine
-
-
-
?
N-acetyl-DL-asparagine + H2O
acetic acid + DL-asparagine
-
low activity
-
?
N-acetyl-DL-methionine + H2O
acetic acid + DL-methionine
-
low activity
-
?
N-acetyl-DL-phenylalanine + H2O
acetic acid + DL-phenylalanine
-
low activity
-
?
N-acetyl-DL-tryptophan + H2O
acetic acid + DL-tryptophan
-
-
-
?
N-acetyl-DL-valine + H2O
acetic acid + DL-valine
-
very low activity
-
?
N-acetyl-glycine + H2O
acetic acid + glycine
-
low activity
-
?
N-acetyl-L-cysteine + H2O
acetic acid + L-cysteine
-
-
-
?
N-acetyl-L-glutamine + H2O
acetic acid + L-glutamine
-
low activity
-
?
N-acetylalanine + H2O
acetate + alanine
-
-
-
-
?
N-acetylglycine + H2O
acetate + glycine
-
-
-
-
?
N-benzoyl-(S-benzoyl)-DL-cysteine + H2O
benzoic acid + S-benzoyl-DL-cysteine
-
low activity
-
?
N-benzoyl-DL-alanine + H2O
benzoic acid + DL-alanine
-
-
-
?
N-benzoyl-DL-alloisoleucine + H2O
benzoic acid + DL-alloisoleucine
-
-
-
?
N-benzoyl-DL-arginine + H2O
benzoic acid + DL-arginine
-
-
-
?
N-benzoyl-DL-asparagine + H2O
benzoic acid + DL-asparagine
-
-
-
?
N-benzoyl-DL-glutamine + H2O
benzoic acid + DL-glutamine
-
-
-
?
N-benzoyl-DL-histidine + H2O
benzoic acid + DL-histidine
-
-
-
?
N-benzoyl-DL-leucine + H2O
benzoic acid + DL-leucine
-
-
-
?
N-benzoyl-DL-methionine + H2O
benzoic acid + DL-methionine
N-benzoyl-DL-phenylalanine + H2O
benzoic acid + DL-phenylalanine
-
-
-
?
N-benzoyl-DL-serine + H2O
benzoic acid + DL-serine
-
-
-
?
N-benzoyl-DL-threonine + H2O
benzoic acid + DL-threonine
-
-
-
?
N-benzoyl-DL-tryptophan + H2O
benzoic acid + DL-tryptophan
-
low activity
-
?
N-benzoyl-DL-tyrosine + H2O
benzoic acid + DL-tyrosine
-
-
-
?
N-benzoyl-DL-valine + H2O
benzoic acid + DL-valine
-
low activity
-
?
N-benzoyl-L-alanine + H2O
benzoic acid + L-alanine
-
-
-
?
N-benzoyl-L-aminobutyric acid + H2O
benzoic acid + L-aminobutyric acid
-
low activity
-
?
N-benzoyl-L-valine + H2O
benzoic acid + L-valine
-
poor substrate
-
?
N-benzoylalanine + H2O
benzoate + alanine
-
-
-
-
?
N-benzoylglutamate + H2O
benzoate + glutamate
-
-
-
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
N-benzoylglycylglycylglycine + H2O
benzoic acid + glycylglycylglycine
-
-
-
?
N-benzoylmethionine + H2O
benzoate + methionine
-
-
-
-
?
N-benzoylphenylalanine + H2O
benzoate + phenylalanine
-
-
-
-
?
N-benzoylvaline + H2O
benzoate + valine
-
-
-
-
?
N-[(4-hydroxyphenyl)carbonyl]glycine + H2O
?
-
-
-
-
?
additional information
?
-
-
substrate specificity, the enzyme also shows metallocarboxypeptidase activity with carboxypeptidase substrates in vitro, displaying its highest activity against N-benzoyl-linked small aliphatic acids, overview, the enzyme does not hydrolyze typical substrates for aminopeptidases, lysine-p-nitroanilide, leucine-p-nitroanilide, or endopeptidases, N-benzoylarginine-p-nitroanilide, succinylphenylalanine-4-nitroanilide
-
-
?
hippurate + H2O

benzoate + glycine
-
-
-
-
?
hippurate + H2O
benzoate + glycine
-
i.e. N-benzoylglycine
-
-
?
N-benzoyl-DL-methionine + H2O

benzoic acid + DL-methionine
-
-
-
?
N-benzoyl-DL-methionine + H2O
benzoic acid + DL-methionine
-
poor substrate
-
?
N-benzoylglycine + H2O

benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
-
-
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
best substrate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
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1,10-phenanthroline
-
99% inhibition at 0.5 mM
2,2'-dipyridyl
-
80% inhibition at 1 mM
2-mercaptoethanol
-
20% inhibition at 2 mM
8-hydroxyquinoline
-
60% inhibition at 3.3 mM
Ag+
-
98% inhibition at 2 mM
Ba2+
-
slight inhibition at 1 mM
Cd2+
-
65% inhibition at 2 mM
EDTA
-
40% inhibition at 10 mM
Fe3+
-
42% inhibition at 1 mM
Mn2+
-
60% inhibition at 1 mM
o-phenanthroline
-
complete inhibition at 1 mM
p-chloromercuribenzoic acid
Zn2+
-
35% inhibition at 2 mM
additional information
-
no significant inhibition by E64, PMSF, and pepstatin at 0.5 mM
-
Ag2+

-
complete inhibition at 1 mM
Ag2+
-
complete inhibition at 1 mM
Ca2+

-
55% inhibition at 2 mM
Ca2+
-
slight inhibition at 1 mM
Co2+

-
70% inhibition at 2 mM
Co2+
-
slight inhibition at 1 mM
Co2+
-
slight inhibition at 1 mM
Cu2+

-
78% inhibition at 2 mM
Cu2+
-
complete inhibition at 1 mM
Cu2+
-
complete inhibition at 1 mM
Fe2+

-
-
Fe2+
-
80% inhibition at 1 mM
Hg2+

-
82% inhibition at 1 mM
Hg2+
-
complete inhibition at 1 mM
iodoacetic acid

-
20% inhibition at 10 mM
iodoacetic acid
-
60% inhibition at 1 mM
Ni2+

-
65% inhibition at 2 mM
Ni2+
-
complete inhibition at 1 mM
p-chloromercuribenzoic acid

-
complete inhibition at 1 mM
p-chloromercuribenzoic acid
-
complete inhibition at 1 mM
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1.1
4-acetyl-N-benzoyl-L-alanine
-
-
0.54
4-acetyl-N-benzoylglycine
-
-
1.769
4-aminobenzoyl-glutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.71
4-chloro-N-benzoyl-L-alanine
-
-
0.37
4-chloro-N-benzoylglycine
-
-
0.95
4-hydroxy-N-benzoyl-L-alanine
-
-
1.25
4-nitro-N-benzoyl-L-alanine
-
-
1.2
4-nitro-N-benzoylglycine
-
-
5.45
N-acetyl-L-alanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
3.377
N-acetylglycine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.87
N-benzoyl-L-alanine
-
-
0.87
N-benzoyl-L-aminobutyric acid
-
-
0.169
N-benzoylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.159
N-benzoylglutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.303
N-benzoylmethionine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.195
N-benzoylphenylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.594
N-benzoylvaline
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.54
N-[(4-hydroxyphenyl)carbonyl]glycine
-
-
0.0006
hippurate

-
at 5°C
0.65
hippurate
-
pH 7.5, 37°C, native enzyme in cell extract
0.764
hippurate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2.778
hippurate
-
pH 7.5, 37°C, recombinant mutant D76N
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14.75
4-aminobenzoyl-glutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2 - 22.12
N-acetyl-L-alanine
0.052 - 10.18
N-acetylglycine
29.38 - 81
N-benzoylalanine
0.17 - 48.72
N-benzoylglutamate
2.6 - 57.13
N-benzoylmethionine
5.95
N-benzoylphenylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.5 - 13.33
N-benzoylvaline
1.4
hippurate

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2.32
hippurate
-
pH 7.5, 37°C, recombinant mutant D76N
98.73
hippurate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2 - 8
N-acetyl-L-alanine

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
22.12
N-acetyl-L-alanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.052 - 2.1
N-acetylglycine

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
10.18
N-acetylglycine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
29.38
N-benzoylalanine

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
81
N-benzoylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.17
N-benzoylglutamate

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
48.72
N-benzoylglutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2.6
N-benzoylmethionine

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
57.13
N-benzoylmethionine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.5
N-benzoylvaline

-
pH 7.5, 37°C, purified recombinant wild-type enzyme
13.33
N-benzoylvaline
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
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