Any feedback?
Information on EC 3.5.1.32 - hippurate hydrolase
for references in articles please use BRENDA:EC3.5.1.32Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.5.1.32 hippurate hydrolaseIUBMB Comments
Acts on various N-benzoylamino acids.
Specify your search results
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
hippuricase, hippurate hydrolase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Benzoylglycine amidohydrolase
-
-
-
-
hippuricase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hippurate + H2O = benzoate + glycine
Acts on various N-benzoylamino acids
-
-
-
hippurate + H2O = benzoate + glycine
residues Asp76, Aps104, Glu134, Glu135, His161 and His356 are involved in zinc-binding and enzyme catalysis
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
N-benzoylamino-acid amidohydrolase
Acts on various N-benzoylamino acids.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37278-43-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-acetyl-N-benzoyl-L-alanine + H2O
4-acetylbenzoic acid + L-alanine
-
low activity
-
?
4-acetyl-N-benzoylglycine + H2O
4-acetylbenzoic acid + glycine
-
-
-
?
4-chloro-N-benzoyl-L-alanine + H2O
4-chlorobenzoic acid + L-alanine
-
-
-
?
4-chloro-N-benzoylglycine + H2O
4-chlorobenzoic acid + glycine
-
-
-
?
4-hydroxy-N-benzoyl-L-alanine
4-hydroxybenzoic acid + L-alanine
-
low activity
-
?
4-hydroxy-N-benzoylglycine + H2O
4-hydroxybenzoic acid + glycine
-
-
-
?
4-hydroxy-N-benzoylglycine-L-histidyl-L-leucine + H2O
4-hydroxybenzoic acid + L-histidyl-L-leucine
-
-
-
?
4-nitro-N-benzoyl-L-alanine + H2O
4-nitrobenzoic acid + L-alanine
-
-
-
?
4-nitro-N-benzoylglycine + H2O
4-nitrobenzoic acid + glycine
-
best substrate
-
?
benzyloxycarbonyl-glycyl-L-phenylalanine + H2O
benzoic acid + ?
-
-
-
?
itoic acid + H2O
2,3-dihydroxybenzoic acid + glycine
-
low specificity
-
?
N-(4-nitrobenzoyl)-Gly-Gly-Gly + H2O
4-nitrobenzoic acid + Gly-Gly-Gly
-
-
-
?
N-acetyl-DL-asparagine + H2O
acetic acid + DL-asparagine
-
low activity
-
?
N-acetyl-DL-methionine + H2O
acetic acid + DL-methionine
-
low activity
-
?
N-acetyl-DL-phenylalanine + H2O
acetic acid + DL-phenylalanine
-
low activity
-
?
N-acetyl-DL-valine + H2O
acetic acid + DL-valine
-
very low activity
-
?
N-acetyl-L-glutamine + H2O
acetic acid + L-glutamine
-
low activity
-
?
N-benzoyl-(S-benzoyl)-DL-cysteine + H2O
benzoic acid + S-benzoyl-DL-cysteine
-
low activity
-
?
N-benzoyl-DL-alloisoleucine + H2O
benzoic acid + DL-alloisoleucine
-
-
-
?
N-benzoyl-DL-asparagine + H2O
benzoic acid + DL-asparagine
-
-
-
?
N-benzoyl-DL-phenylalanine + H2O
benzoic acid + DL-phenylalanine
-
-
-
?
N-benzoyl-DL-tryptophan + H2O
benzoic acid + DL-tryptophan
-
low activity
-
?
N-benzoyl-DL-valine + H2O
benzoic acid + DL-valine
-
low activity
-
?
N-benzoyl-L-aminobutyric acid + H2O
benzoic acid + L-aminobutyric acid
-
low activity
-
?
N-benzoyl-L-valine + H2O
benzoic acid + L-valine
-
poor substrate
-
?
N-benzoylglycylglycylglycine + H2O
benzoic acid + glycylglycylglycine
-
-
-
?
additional information
?
-
-
substrate specificity, the enzyme also shows metallocarboxypeptidase activity with carboxypeptidase substrates in vitro, displaying its highest activity against N-benzoyl-linked small aliphatic acids, overview, the enzyme does not hydrolyze typical substrates for aminopeptidases, lysine-p-nitroanilide, leucine-p-nitroanilide, or endopeptidases, N-benzoylarginine-p-nitroanilide, succinylphenylalanine-4-nitroanilide
-
-
?
N-benzoyl-DL-methionine + H2O
benzoic acid + DL-methionine
-
-
-
?
N-benzoyl-DL-methionine + H2O
benzoic acid + DL-methionine
-
poor substrate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
best substrate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
N-benzoylglycine + H2O
benzoic acid + glycine
-
hippurate
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Zn2+
-
1.98 molecules of zinc per enzyme subunit molecule, metalloenzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no significant inhibition by E64, PMSF, and pepstatin at 0.5 mM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Campylobacter Infections
The detection of hipO gene by real-time PCR in thermophilic Campylobacter spp. with very weak and negative reaction of hippurate hydrolysis.
Crohn Disease
Molecular detection of Campylobacter jejuni in patients with Crohn's disease in Iran.
Diabetic Foot
Enzymatically active Peptostreptococcus magnus: association with site of infection.
Infections
Enzymatically active Peptostreptococcus magnus: association with site of infection.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.769
4-aminobenzoyl-glutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
5.45
N-acetyl-L-alanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
3.377
N-acetylglycine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.169
N-benzoylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.159
N-benzoylglutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.303
N-benzoylmethionine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.195
N-benzoylphenylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.594
N-benzoylvaline
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
14.75
4-aminobenzoyl-glutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
5.95
N-benzoylphenylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2 - 8
N-acetyl-L-alanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
22.12
N-acetyl-L-alanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.052 - 2.1
N-acetylglycine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
10.18
N-acetylglycine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
29.38
N-benzoylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
81
N-benzoylalanine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.17
N-benzoylglutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
48.72
N-benzoylglutamate
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
2.6
N-benzoylmethionine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
57.13
N-benzoylmethionine
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
0.5
N-benzoylvaline
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
13.33
N-benzoylvaline
-
pH 7.5, 37°C, purified recombinant wild-type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
induction of enzyme synthesis by hippurate-containing media
brenda