Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
L-glutamine | recombinant enzyme with N-terminal and C-terminal deletions, T-phosphate buffer, pH 8.0 | Rattus norvegicus | |
3.6 | - |
L-glutamine | recombinant enzyme with N-terminal deletion, T-phosphate buffer, pH 8.0 | Rattus norvegicus | |
6.4 | - |
L-glutamine | recombinant enzyme with N-terminal and C-terminal deletions, T-acetate buffer, pH 8.0 | Rattus norvegicus | |
7.9 | - |
L-glutamine | recombinant enzyme with N-terminal deletion, T-acetate buffer, pH 8.0 | Rattus norvegicus | |
8.1 | - |
L-glutamine | native enzyme, T-phosphate buffer, pH 8.0 | Rattus norvegicus | |
14 | - |
L-glutamine | native enzyme, T-acetate buffer, pH 8.0 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | induces association to form active tetramers | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
recombinant enzyme with N-terminal and C-terminal deletions, SDS-PAGE | Rattus norvegicus |
49700 | - |
recombinant enzyme with N-terminal and C-terminal deletions, based on amino acid content | Rattus norvegicus |
63200 | - |
4 * 63200, SDS-PAGE, recombinant enzyme with N-terminal deletion | Rattus norvegicus |
64000 | - |
4 * 64000, based on amino acid content, recombinant enzyme with N-terminal deletion | Rattus norvegicus |
260000 | - |
recombinant enzyme with N-terminal deletion dialyzed versus T-phosphate buffer, pH 8.0, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Rattus norvegicus | renal ammonia genesis and the utilization of glutamine as a metabolic fuel, neuronal synthesis of glutamate | L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | in mitochondria, the first 16 amino acids are removed | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant enzyme with N-terminal deletion and recombinant enzyme with N-terminal and C-terminal deletions, by nickel-affinity chromatography, 63fold and 113fold respectively | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
403 | - |
recombinant enzyme with N-terminal deletion | Rattus norvegicus |
405 | - |
recombinant enzyme with N-terminal and C-terminal deletions | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4ºC, T-phosphate buffer, pH 8.0, 1 mM dithiothreitol, very stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Rattus norvegicus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | renal ammonia genesis and the utilization of glutamine as a metabolic fuel, neuronal synthesis of glutamate | Rattus norvegicus | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 63200, SDS-PAGE, recombinant enzyme with N-terminal deletion | Rattus norvegicus |
tetramer | 4 * 64000, based on amino acid content, recombinant enzyme with N-terminal deletion | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
KGA | - |
Rattus norvegicus |
kidney-type-glutaminase | - |
Rattus norvegicus |