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Literature summary for 3.5.1.137 extracted from

  • Jiang, W.; Liu, Y.; Ke, Z.; Zhang, L.; Zhang, M.; Zhou, Y.; Wang, H.; Wu, C.; Qiu, J.; Hong, Q.
    Substrate preference of carbamate hydrolase CehA reveals its environmental behavior (2021), J. Hazard. Mater., 403, 123677 .
    View publication on PubMed
Search for more literature for 3.5.1.137

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingobium sp. CFD-1
expressed in Escherichia coli BL21(DE3) cells Rhizobium sp. AC100
expressed in Escherichia coli BL21(DE3) cells Novosphingobium sp. KN65.2
expressed in Escherichia coli BL21(DE3) cells Sphingomonas sp. CDS-1
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. OXA20

Protein Variants

Protein Variants Comment Organism
F152L the mutation determines the catalytic preference for bicyclic carbamate substrates (carbofuran, carbaryl) Sphingobium sp. CFD-1
F152L the mutation determines the catalytic preference for bicyclic carbamate substrates (carbofuran, carbaryl) Rhizobium sp. AC100
F152L the mutation determines the catalytic preference for bicyclic carbamate substrates (carbofuran, carbaryl) Novosphingobium sp. KN65.2
F152L the mutation determines the catalytic preference for bicyclic carbamate substrates (carbofuran, carbaryl) Sphingomonas sp. CDS-1
F152L the mutation determines the catalytic preference for bicyclic carbamate substrates (carbofuran, carbaryl) Pseudomonas sp. OXA20
T494A the mutation determines the preference for monocyclic carbamate substrates (isoprocarb, propoxur) Sphingobium sp. CFD-1
T494A the mutation determines the preference for monocyclic carbamate substrates (isoprocarb, propoxur) Rhizobium sp. AC100
T494A the mutation determines the preference for monocyclic carbamate substrates (isoprocarb, propoxur) Novosphingobium sp. KN65.2
T494A the mutation determines the preference for monocyclic carbamate substrates (isoprocarb, propoxur) Sphingomonas sp. CDS-1
T494A the mutation determines the preference for monocyclic carbamate substrates (isoprocarb, propoxur) Pseudomonas sp. OXA20
T570I the mutation determines the preference for linear carbamate substrates (oxamyl, aldicarb) Sphingobium sp. CFD-1
T570I the mutation determines the preference for linear carbamate substrates (oxamyl, aldicarb) Rhizobium sp. AC100
T570I the mutation determines the preference for linear carbamate substrates (oxamyl, aldicarb) Novosphingobium sp. KN65.2
T570I the mutation determines the preference for linear carbamate substrates (oxamyl, aldicarb) Sphingomonas sp. CDS-1
T570I the mutation determines the preference for linear carbamate substrates (oxamyl, aldicarb) Pseudomonas sp. OXA20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0813
-
 Carbaryl at pH 7.5 and 30°C Pseudomonas sp. OXA20
0.0864
-
 carbofuran at pH 7.5 and 30°C Novosphingobium sp. KN65.2
0.0877
-
 carbofuran at pH 7.5 and 30°C Pseudomonas sp. OXA20
0.1088
-
 Carbaryl at pH 7.5 and 30°C Rhizobium sp. AC100
0.1113
-
 Carbaryl at pH 7.5 and 30°C Sphingobium sp. CFD-1
0.1233
-
 Carbaryl at pH 7.5 and 30°C Sphingomonas sp. CDS-1
0.1332
-
 carbofuran at pH 7.5 and 30°C Sphingobium sp. CFD-1
0.1472
-
 oxamyl at pH 7.5 and 30°C Sphingomonas sp. CDS-1
0.1676
-
 Carbaryl at pH 7.5 and 30°C Novosphingobium sp. KN65.2
0.1988
-
 oxamyl at pH 7.5 and 30°C Novosphingobium sp. KN65.2
0.2044
-
 oxamyl at pH 7.5 and 30°C Pseudomonas sp. OXA20
0.2207
-
 oxamyl at pH 7.5 and 30°C Rhizobium sp. AC100
0.2304
-
 carbofuran at pH 7.5 and 30°C Sphingomonas sp. CDS-1
0.88
-
 carbofuran at pH 7.5 and 30°C Rhizobium sp. AC100
1.5803
-
 oxamyl at pH 7.5 and 30°C Sphingobium sp. CFD-1

Organism

Organism UniProt Comment Textmining
Novosphingobium sp. KN65.2 A0A158SQ52
-
-
Pseudomonas sp. OXA20
-
-
-
Rhizobium sp. AC100 Q8RR61
-
-
Sphingobium sp. CFD-1 A0A6B9IL91
-
-
Sphingomonas sp. CDS-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography Sphingobium sp. CFD-1
Ni-NTA agarose resin column chromatography Rhizobium sp. AC100
Ni-NTA agarose resin column chromatography Novosphingobium sp. KN65.2
Ni-NTA agarose resin column chromatography Sphingomonas sp. CDS-1
Ni-NTA agarose resin column chromatography Pseudomonas sp. OXA20

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldicarb + H2O
-
 Sphingobium sp. CFD-1 ?
-
 ?
aldicarb + H2O
-
 Rhizobium sp. AC100 ?
-
 ?
aldicarb + H2O
-
 Novosphingobium sp. KN65.2 ?
-
 ?
aldicarb + H2O
-
 Sphingomonas sp. CDS-1 ?
-
 ?
aldicarb + H2O
-
 Pseudomonas sp. OXA20 ?
-
 ?
carbaryl + H2O
-
 Sphingobium sp. CFD-1 1-naphthol + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Rhizobium sp. AC100 1-naphthol + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Novosphingobium sp. KN65.2 1-naphthol + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Sphingomonas sp. CDS-1 1-naphthol + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Pseudomonas sp. OXA20 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O
-
 Sphingobium sp. CFD-1 ?
-
 ?
carbofuran + H2O
-
 Rhizobium sp. AC100 ?
-
 ?
carbofuran + H2O
-
 Novosphingobium sp. KN65.2 ?
-
 ?
carbofuran + H2O
-
 Sphingomonas sp. CDS-1 ?
-
 ?
carbofuran + H2O
-
 Pseudomonas sp. OXA20 ?
-
 ?
isoprocarb + H2O
-
 Sphingobium sp. CFD-1 ?
-
 ?
isoprocarb + H2O
-
 Rhizobium sp. AC100 ?
-
 ?
isoprocarb + H2O
-
 Novosphingobium sp. KN65.2 ?
-
 ?
isoprocarb + H2O
-
 Sphingomonas sp. CDS-1 ?
-
 ?
isoprocarb + H2O
-
 Pseudomonas sp. OXA20 ?
-
 ?
oxamyl + H2O
-
 Sphingobium sp. CFD-1 ?
-
 ?
oxamyl + H2O
-
 Rhizobium sp. AC100 ?
-
 ?
oxamyl + H2O
-
 Novosphingobium sp. KN65.2 ?
-
 ?
oxamyl + H2O
-
 Sphingomonas sp. CDS-1 ?
-
 ?
oxamyl + H2O
-
 Pseudomonas sp. OXA20 ?
-
 ?
propoxur + H2O
-
 Sphingobium sp. CFD-1 ?
-
 ?
propoxur + H2O
-
 Rhizobium sp. AC100 ?
-
 ?
propoxur + H2O
-
 Novosphingobium sp. KN65.2 ?
-
 ?
propoxur + H2O
-
 Sphingomonas sp. CDS-1 ?
-
 ?
propoxur + H2O
-
 Pseudomonas sp. OXA20 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Sphingobium sp. CFD-1
? x * 90000, SDS-PAGE Rhizobium sp. AC100
? x * 90000, SDS-PAGE Novosphingobium sp. KN65.2
? x * 90000, SDS-PAGE Sphingomonas sp. CDS-1
? x * 90000, SDS-PAGE Pseudomonas sp. OXA20

Synonyms

Synonyms Comment Organism
carbamate hydrolase
-
 Sphingobium sp. CFD-1
carbamate hydrolase
-
 Rhizobium sp. AC100
carbamate hydrolase
-
 Novosphingobium sp. KN65.2
carbamate hydrolase
-
 Sphingomonas sp. CDS-1
carbamate hydrolase
-
 Pseudomonas sp. OXA20
cehA
-
 Sphingobium sp. CFD-1
cehA
-
 Rhizobium sp. AC100
cehA
-
 Novosphingobium sp. KN65.2
cehA
-
 Sphingomonas sp. CDS-1
cehA
-
 Pseudomonas sp. OXA20

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 oxamyl at pH 7.5 and 30°C Sphingobium sp. CFD-1
0.6
-
 carbofuran at pH 7.5 and 30°C Pseudomonas sp. OXA20
0.7
-
 carbofuran at pH 7.5 and 30°C Rhizobium sp. AC100
2.7
-
 oxamyl at pH 7.5 and 30°C Sphingomonas sp. CDS-1
4.1
-
 oxamyl at pH 7.5 and 30°C Novosphingobium sp. KN65.2
4.9
-
 oxamyl at pH 7.5 and 30°C Rhizobium sp. AC100
5.8
-
 oxamyl at pH 7.5 and 30°C Pseudomonas sp. OXA20
9.5
-
 carbofuran at pH 7.5 and 30°C Sphingobium sp. CFD-1
10.2
-
 carbofuran at pH 7.5 and 30°C Sphingomonas sp. CDS-1
19.6
-
 carbofuran at pH 7.5 and 30°C Novosphingobium sp. KN65.2
92.5
-
 Carbaryl at pH 7.5 and 30°C Sphingomonas sp. CDS-1
100.5
-
 Carbaryl at pH 7.5 and 30°C Sphingobium sp. CFD-1
636.4
-
 Carbaryl at pH 7.5 and 30°C Pseudomonas sp. OXA20
955.9
-
 Carbaryl at pH 7.5 and 30°C Rhizobium sp. AC100
1171.2
-
 Carbaryl at pH 7.5 and 30°C Novosphingobium sp. KN65.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
 oxamyl at pH 7.5 and 30°C Sphingobium sp. CFD-1
0.81
-
 carbofuran at pH 7.5 and 30°C Rhizobium sp. AC100
4
-
 carbofuran at pH 7.5 and 30°C Sphingomonas sp. CDS-1
7.1
-
 carbofuran at pH 7.5 and 30°C Pseudomonas sp. OXA20
19
-
 oxamyl at pH 7.5 and 30°C Sphingomonas sp. CDS-1
21
-
 oxamyl at pH 7.5 and 30°C Novosphingobium sp. KN65.2
22
-
 oxamyl at pH 7.5 and 30°C Rhizobium sp. AC100
28
-
 oxamyl at pH 7.5 and 30°C Pseudomonas sp. OXA20
71
-
 carbofuran at pH 7.5 and 30°C Sphingobium sp. CFD-1
270
-
 carbofuran at pH 7.5 and 30°C Novosphingobium sp. KN65.2
800
-
 Carbaryl at pH 7.5 and 30°C Sphingomonas sp. CDS-1
900
-
 Carbaryl at pH 7.5 and 30°C Sphingobium sp. CFD-1
6900
-
 Carbaryl at pH 7.5 and 30°C Novosphingobium sp. KN65.2
7800
-
 Carbaryl at pH 7.5 and 30°C Pseudomonas sp. OXA20
8800
-
 Carbaryl at pH 7.5 and 30°C Rhizobium sp. AC100