Literature summary for 3.5.1.137 extracted from

Chaudhry, G.R.; Mateen, A.; Kaskar, B.; Bloda, M.; Riazuddin, S.
Purification and biochemical characterization of the carbamate hydrolase from Pseudomonas sp. 50432 (2002), Biotechnol. Appl. Biochem., 36, 63-70 .

Search for more literature for 3.5.1.137

General Stability

General Stability	Organism
the enzyme is stable in Triton X-100 and other detergents such as CHAPS	Pseudomonas sp. 50432

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	the enzyme shows 10% activity in the presence of 0.1% (v/v) 2-mercaptoethanol. Higher concentrations completely inactivate the enzyme. However, the activity of the enzyme is recovered when 2-mercaptoethanol was removed by dialysis	Pseudomonas sp. 50432
dithiothreitol	the enzyme shows 10% activity in the presence of 0.1% (v/v) dithiothreitol. Higher concentrations completely inactivate the enzyme	Pseudomonas sp. 50432
additional information	EDTA does not affect the enzyme activity	Pseudomonas sp. 50432

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	Carbaryl	at pH 7.0 and 25°C	Pseudomonas sp. 50432
0.016	-	carbofuran	at pH 7.0 and 25°C	Pseudomonas sp. 50432

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Pseudomonas sp. 50432	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	cations do not affect the enzyme activity	Pseudomonas sp. 50432

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88000	-	gel filtration	Pseudomonas sp. 50432

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. 50432	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, and Macro-prep tert-butyl column chromatography	Pseudomonas sp. 50432

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.5	-	crude cell extract, at pH 7.0 and 25°C	Pseudomonas sp. 50432
1016	-	after 2032fold purification, at pH 7.0 and 25°C	Pseudomonas sp. 50432

Storage Stability

Storage Stability	Organism
-20 or -70°C, purified enzyme in 10 mM K2HPO4 (pH 7.5) in the presence of 20% (v/v) glycerol, 1 month, no loss of activity	Pseudomonas sp. 50432
4°C, purified enzyme in 10 mM K2HPO4 (pH 7.5), 1 month, 25% loss of activity	Pseudomonas sp. 50432
4°C, purified enzyme in 10 mM K2HPO4 (pH 7.5), 1 week, no loss of activity	Pseudomonas sp. 50432

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-naphthyl acetate + H2O	-	Pseudomonas sp. 50432	1-naphthol + acetate	-	?
carbaryl + H2O	-	Pseudomonas sp. 50432	1-naphthol + methylamine + CO2	-	?
carbofuran + H2O	-	Pseudomonas sp. 50432	7-phenol + methylamine + CO2	-	?
additional information	the purified enzyme does not hydrolyze 2-nitrophenyl dimethylcarbamate	Pseudomonas sp. 50432	?	-	-

Subunits

Subunits	Comment	Organism
monomer	1 * 88000, SDS-PAGE	Pseudomonas sp. 50432

Synonyms

Synonyms	Comment	Organism
carbamate hydrolase	-	Pseudomonas sp. 50432
carbofuran hydrolase	-	Pseudomonas sp. 50432

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Pseudomonas sp. 50432

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	when heated at 60°C for 5 min, the catalytic activity of the enzyme is completely lost	Pseudomonas sp. 50432

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Pseudomonas sp. 50432

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas sp. 50432	isoelectric focusing	-	6.9

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Release 2023.1 (January 2023)