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Literature summary for 3.5.1.137 extracted from

  • Mulbry, W.W.; Eaton, R.W.
    Purification and characterization of the N-methylcarbamate hydrolase from Pseudomonas strain CRL-OK (1991), Appl. Environ. Microbiol., 57, 3679-3682 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.137

Activating Compound

Activating Compound Comment Organism Structure
additional information hydrolase activity is not affected by the presence or absence of sulfhydryl reagents such as 2-mercaptoethanol (more than 90% activity in assays containing up to 0.2 mM 2-mercaptoethanol). The enzyme activity is unaffected in assays containing up to 10 mM EDTA Pseudomonas sp. CRL-OK
SDS hydrolase activity is slightly enhanced by low concentrations of SDS and retains greater than 70% of its activity in assay mixtures containing up to 0.5% (w/v) SDS Pseudomonas sp. CRL-OK

General Stability

General Stability Organism
enzyme activity is not diminished after repeated cycles of freezing and thawing Pseudomonas sp. CRL-OK

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114
-
 carbofuran at pH 7.0, temperature not specified in the publication Pseudomonas sp. CRL-OK
0.213
-
 Carbaryl at pH 7.0, temperature not specified in the publication Pseudomonas sp. CRL-OK
0.542
-
 Aldicarb at pH 7.0, temperature not specified in the publication Pseudomonas sp. CRL-OK

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pseudomonas sp. CRL-OK 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
187000
-
 sucrose density gradient centrifugation Pseudomonas sp. CRL-OK

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. CRL-OK
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column chromatography, TSK phenyl column chromatography and 300SW gel filtration Pseudomonas sp. CRL-OK

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.013
-
 crude soluble extract, at pH 7.0, temperature not specified in the publication Pseudomonas sp. CRL-OK
14.1
-
 after 1085fold purification, at pH 7.0, temperature not specified in the publication Pseudomonas sp. CRL-OK

Storage Stability

Storage Stability Organism
-80°C, crude extract and purified enzyme, more than 1 month, no loss of activity Pseudomonas sp. CRL-OK
4°C, after partial purification by DEAE column chromatography, more than 1 month, no loss of activity Pseudomonas sp. CRL-OK
4°C, crude extract, 24 h, 30% loss of activity Pseudomonas sp. CRL-OK

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldicarb + H2O i.e. 2-methyl-2-(methylthio)propionaldehyde O-(methylcarbamoyl)oxime Pseudomonas sp. CRL-OK ? + methylamine + CO2
-
 ?
carbaryl + H2O i.e. 1-naphthyl N-methylcarbamate Pseudomonas sp. CRL-OK 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O i.e. 2,3-dihydro-2,2-dimethyl-7-benzofuranyl N-methylcarbamate Pseudomonas sp. CRL-OK ? + methylamine + CO2
-
 ?
additional information no activity with isopropyl 3-chlorocarbanilate, S-ethyl N,N-dipropylthiocarbamate, and 2-nitrophenyldimethylcarbamate Pseudomonas sp. CRL-OK ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 85000, SDS-PAGE Pseudomonas sp. CRL-OK

Synonyms

Synonyms Comment Organism
N-methylcarbamate hydrolase
-
 Pseudomonas sp. CRL-OK

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudomonas sp. CRL-OK

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Pseudomonas sp. CRL-OK