Literature summary for 3.5.1.11 extracted from

Ye, J.; Li, A.; Chu, T.; Pan, X.; He, B.
Poly-lysine supported cross-linked enzyme aggregates of penicillin G acylase and its application in synthesis of beta-lactam antibiotics (2019), Int. J. Biol. Macromol., 140, 423-428 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) using the pET-26b(+) expression vector	Providencia rettgeri

General Stability

General Stability	Organism
amoxicillin synthesis activity of cross-linked enzyme aggregates and poly-lysine supported cross-linked enzyme aggregates is retained over twenty cycles. The amoxicillin synthesis activity of cross-linked enzyme aggregates gradually decreases. After twenty cycles, 73% of initial activity is retained. Poly-lysine supported cross-linked enzyme aggregates achieve excellent operational stability spanning twenty cycles. Poly-lysine supported cross-linked enzyme aggregates retain 97% of their initial activity after twenty conversion cycles	Providencia rettgeri

Organism

amoxicillin synthesis activity of cross-linked enzyme aggregates and poly-lysine supported cross-linked enzyme aggregates is retained over twenty cycles. The amoxicillin synthesis activity of cross-linked enzyme aggregates gradually decreases. After twenty cycles, 73% of initial activity is retained. Poly-lysine supported cross-linked enzyme aggregates achieve excellent operational stability spanning twenty cycles. Poly-lysine supported cross-linked enzyme aggregates retain 97% of their initial activity after twenty conversion cycles

Providencia rettgeri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.9919	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
1.007	-	amoxicillin	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
1.6723	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
7.31	-	amoxicillin	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
7.9	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
11.74	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
30.39	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
41.26	-	amoxicillin	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
51.15	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri

Organism

Organism	UniProt	Comment	Textmining
Providencia rettgeri	A0A2I6UI54	-	-
Providencia rettgeri PX04	A0A2I6UI54	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O	-	Providencia rettgeri	phenylacetic acid + 5-amino-2-nitrobenzoic acid	-	?
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O	-	Providencia rettgeri PX04	phenylacetic acid + 5-amino-2-nitrobenzoic acid	-	?
amoxicillin + H2O	-	Providencia rettgeri	D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid	-	?
amoxicillin + H2O	-	Providencia rettgeri PX04	D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid	-	?
D-hydroxyphenylglycine methyl ester + H2O	-	Providencia rettgeri	?	-	?
D-hydroxyphenylglycine methyl ester + H2O	-	Providencia rettgeri PX04	?	-	?

Synonyms

Synonyms	Comment	Organism
penicillin G acylase	-	Providencia rettgeri

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	soluble enzyme	Providencia rettgeri
50	-	poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	20°C: about 75% of maximal activity, 50°C: about 90% of maximal activity, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
20	50	20°C: about 80% of maximal activity, 50°C: about 70% of maximal activity, soluble enzyme	Providencia rettgeri

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
11.61	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
11.73	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
14.67	-	amoxicillin	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
15.7	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
16.9	-	amoxicillin	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
17.6	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
102.6	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
142.3	-	amoxicillin	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
145.3	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	soluble enzyme	Providencia rettgeri
10	-	poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	11	pH 6.0: about 70% of maximal activity, pH 11.0: about 75% of maximal activity, soluble enzyme	Providencia rettgeri
6	11	pH 6.0: about 75% of maximal activity, pH 11.0: about 90% of maximal activity, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.22	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
0.356	-	amoxicillin	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
0.52	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, cross-linked enzyme aggregates	Providencia rettgeri
6.94	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
8.74	-	D-hydroxyphenylglycine methyl ester	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
16.78	-	amoxicillin	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
17.74	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, soluble enzyme	Providencia rettgeri
18.39	-	2-nitro-5-[(phenylacetyl)amino]-benzoic acid	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri
19.47	-	amoxicillin	pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates	Providencia rettgeri