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(+)-methyl mandelate + H2O
?
-
-
-
-
?
(R) -2-(4-hydroxyphenyl)glycine amide + H2O
(R) -2-(4-hydroxyphenyl)glycine + hydroxylamine
-
-
-
?
(R)-2-phenylglycine amide + H2O
(R)-2-phenylglycine + hydroxylamine
(R)-2-phenylglycine methyl ester + H2O
(R)-2-phenylglycine + methanol
(R)-beta-phenylalanine + phenylacetamide
N-phenylacetyl-(R)-beta-phenylalanine + ?
-
the acylation reaction is highly preferential for the acylation of (R)-beta-phenylalanine
-
-
?
(R)-mandelic acid methyl ester + H2O
?
-
-
-
-
?
(R)-N-acetylphenylglycine + H2O
?
-
S-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C
-
?
(R)-p-hydroxyphenylglycinamide + H2O
?
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C
-
?
(R)-phenylglycinamide + H2O
?
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C
-
?
(S)-N-acetylphenylglycine + H2O
?
-
S-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C
-
?
(S)-p-hydroxyphenylglycinamide + H2O
?
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C
-
?
(S)-phenylglycinamide + H2O
?
-
R-specific, the stereoselectivity of the reaction decreases almost by one order of magnitude from 5°C to 45°C
-
?
2-furylmethylpenicillin + H2O
6-aminopenicillanate + furan-2-yl-acetic acid
-
-
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
5-amino-2-nitrobenzoic acid + phenoxyacetate
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
?
-
-
-
?
2-nitro-5-phenoxyacetamide benzoic acid + H2O
?
-
-
-
?
2-nitro-5-phenylacetamidobenzoic acid + H2O
phenylacetate + 2-aminobenzoic acid
-
-
-
-
?
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid + H2O
?
-
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O
5-amino-2-nitrobenzoic acid + ?
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
2-phenyl-N-(4-sulfamoylphenyl)acetamide + H2O
phenylacetate + 2-amino-4-sulfamoylphenol
-
-
-
-
?
2-phenylacetamidobenzoic acid + H2O
phenylacetate + 2-aminobenzoic acid
-
-
-
-
?
2-thienylmethylpenicillin + H2O
6-aminopenicillanate + sulfo-(5-sulfothiophen-2-yl)-acetic acid
-
-
-
-
?
3-nitro-6-phenylacetaminobenzoic acid + H2O
6-nitrophenol + ?
-
-
-
-
?
3-nitro-6-phenylacetaminobenzoic acid + H2O
?
3-phenylacetamidobenzoic acid + H2O
phenylacetate + 3-aminobenzoic acid
-
-
-
-
?
4-chloro-L-phenylalanine + phenylacetamide
N-[(4-chlorophenyl)acetyl]-L-tyrosine + NH3
-
-
-
-
?
4-fluoro-L-phenylalanine + phenylacetamide
N-[(4-fluorophenyl)acetyl]-L-tyrosine + NH3
-
-
-
-
?
4-hydroxyphenylacetamide + 6-aminopenicillanic acid
?
4-methyl-L-phenylalanine + phenylacetamide
N-[(4-methylphenyl)acetyl]-L-tyrosine + NH3
-
-
-
-
?
4-nitro-L-phenylalanine + phenylacetamide
N-[(4-nitrophenyl)acetyl]-L-tyrosine + NH3
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-phenylacetamidobenzoic acid + H2O
phenylacetate + 4-aminobenzoic acid
-
-
-
-
?
5-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetate + 3-amino-5-nitro-benzoic acid
-
-
-
-
?
5-[[(2R)-2-amino-2-phenylethanoyl]amino]-2-nitrobenzoic acid + H2O
5-amino-2-nitrobenzoic acid + (R)-2-phenylglycine
-
-
-
?
6-aminopenicillanate + phenylacetic acid
penicillin G + H2O
6-aminopenicillanic acid + methyl n-hexanoate
dihydropenicillin F + methanol
-
-
-
-
?
6-aminopenicillanic acid + methyl n-octanoate
penicillin K + methanol
-
-
-
-
?
6-aminopenicillanic acid + p-hydroxyphenylglycine methyl ester
amoxicillin + methanol
-
-
-
-
?
6-aminopenicillanic acid + phenoxyacetic acid
penicillin V + H2O
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
6-nitro-3-(phenylacetamido)benzoic acid + H2O
3-amino-6-nitrobenzoic acid + ?
-
-
-
-
?
6-nitro-3-(phenylacetamido)benzoic acid + H2O
?
6-nitro-3-(phenylacetamido)benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
6-nitro-3-phenylacetamide benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-phenylacetamide benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
6-nitro-3-phenylacetamido-benzoic acid + H2O
?
6-nitro-3-phenylacetamidobenzoic acid + H2O
?
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
7-amino-3-deacetoxycephalosporanic acid + phenylglycine methyl ester
cephalexin + ?
-
-
-
-
?
7-amino-3-propenyl-cephalosporanic acid + 4-hydroxy-D-phenylglycine methyl ester
cefprozil + ?
-
-
-
?
7-amino-deacetoxycephalosporanic acid + phenylacetic acid
deacetoxycephalosporin G + H2O
-
-
-
-
?
7-amino-desacetoxycephalosporanic acid + D-alpha-phenylglycine methylester
?
-
acylating activity
-
-
?
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide
cephalexin
-
high reactant concentrations
-
-
r
7-aminocephalosporanic acid + (R)-mandelic acid
7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-D3-cephem-4-carboxylic acid
-
-
-
-
?
7-aminocephalosporanic acid + D-alpha-phenylglycine methylester
?
-
acylating activity
-
-
?
7-aminodeacetoxycephalosporanic acid + (R)-2-phenylglycine methylester
cephalexin + ?
-
-
-
-
?
7-aminodeacetoxycephalosporanic acid + 2-benzoxazolon-3-yl-acetic acid methyl ester
?
-
-
-
?
7-aminodeacetoxycephalosporanic acid + phenoxyacetic acid
deacetoxycephalosporin V + H2O
-
-
-
-
?
7-aminodeacetoxycephalosporanic acid + phenylacetic acid methyl ester
?
-
-
-
?
7-phenylacetamidoacetoxycephalosporanic acid + H2O
?
-
-
-
-
?
aculeacin A + H2O
?
recombinant enzyme
-
-
?
allopurinol + vinyl acetate
1-(4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl acetate
-
Markovnikov addition, decrease in activity in the order vinyl acetate, vinyl pentanoate, vinyl decanoate
-
-
?
allopurinol + vinyl decanoate
1-(4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl decanoate
-
Markovnikov addition, decrease in activity in the order vinyl acetate, vinyl pentanoate, vinyl decanoate
-
-
?
allopurinol + vinyl pentanoate
1-(4-oxo-4,5-dihydro-1H-pyrazolo[3,4-d]pyrimidin-1-yl)ethyl pentanoate
-
Markovnikov addition, decrease in activity in the order vinyl acetate, vinyl pentanoate, vinyl decanoate
-
-
?
allylmercaptomethylpenicillin + H2O
?
-
poor substrate
-
-
?
amoxicillin + H2O
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
amoxillin + H2O
D-4-hydroxyphenylglycine + 6-aminopenicillanic acid
amoxycillin + H2O
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
-
-
?
ampicillin + H2O
(R)-2-phenylglycine + 6-aminopenicillanic acid
ampicillin + H2O
6-aminopenicillanic acid + (R)-2-phenylglycine
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
benzylpenicilloic acid + H2O
?
-
-
-
-
?
beta-(alpha-toluylamido)-propionic acid + H2O
?
-
poor substrate
-
-
?
capsaicin + H2O
?
-
-
-
?
carbenicillin + H2O
phenylpropanedioate + 6-aminopenicillate
-
-
-
?
cefamandole + H2O
(6R,7R)-7-amino-3-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-hydroxy(phenyl)acetic acid
-
-
-
-
?
cephadrine + H2O
?
Pseudomonas melanogenum
-
-
-
-
?
cephalexin + H2O
(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
-
-
-
?
cephalexin + H2O
7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine
cephalexin + H2O
7-aminodesacetoxycephalosporanic acid + D-phenylglycine
cephalexin + H2O
D-phenylglycine + 7-aminodesacetoxycephalosporanic acid
-
-
-
-
r
cephaloglycin + H2O
(6R,7R)-7-amino-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-amino(phenyl)acetic acid
Pseudomonas melanogenum
-
-
-
-
?
cephaloglycin + H2O
?
-
-
-
-
?
cephaloridine + H2O
(6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
cephaloridine + H2O
thiophen-2-ylacetic acid + (6R,7R)-7-amino-8-oxo-3-(pyridinium-1-yl-methyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephalosporanic acid V + H2O
?
-
-
-
-
?
cephalosporin + H2O
7-aminocephalosporanic acid + an amino acid
cephalosporin C + H2O
7-aminocephalosporanic acid + (R)-2-aminohexadioic acid
-
2.4% of the activity compared to penicillin V
-
-
?
cephalosporin G + H2O
7-amino-desacetoxycephalosporanic acid + phenylacetic acid
-
-
-
-
?
cephalosporin G + H2O
7-aminodeacetoxycephalosporanate + phenylacetic acid
-
-
-
-
r
cephalosporin G + H2O
phenylacetic acid + 7-aminodeacetoxycephalosporanate
-
-
-
-
?
cephalothin + H2O
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
cephalothin + H2O
thiophen-2-ylacetic acid + (6R,7R)-3-acetoxymethyl-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephradine + H2O
(2R)-amino(cyclohexa-1,4-dien-1-yl)acetic acid + (6R,7R)-7-amino-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
-
?
D,L-N-phenylacetylalanine + H2O
?
-
poor substrate
-
-
?
D-(-)-alpha-aminophenylacetic acid 4-nitroanilide + H2O
D-(-)-alpha-aminophenylacetic acid + 4-nitrophenol
-
-
-
-
?
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
D-2-nitro-5[(phenylglycyl)amino]benzoic acid + H2O
?
-
-
-
-
?
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
amoxicillin + NH3
D-alpha-aminobenzylpenicillin + H2O
6-aminopenicillanate + DL-alpha-aminophenylacetic acid
-
-
-
?
D-hydroxyphenylglycine methyl ester + H2O
?
D-phenylglycine amide + 6-aminopenicillanic acid
ampicillin + H2O
D-phenylglycine amide + 6-aminopenicillanic acid
ampicillin + NH3
-
-
-
-
r
D-phenylglycine amide + 7-aminodeacetoxycephalosporanic acid
cephalexin + ?
-
-
-
?
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin + NH3
-
-
-
-
r
D-phenylglycine amide + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
cephaclor + ?
-
-
-
?
D-phenylglycine amide + H2O
D-phenylglycine + NH3
-
-
-
-
?
D-phenylglycine methyl ester + 6-aminopenicillanic acid
methanol + ampicillin
-
-
-
r
D-phenylglycine methyl ester + 6-aminopenicillic acid
ampicillin + methanol
-
high ratio of synthesis to hydrolysis at up to 200 mM 6-aminopenicillic acid and 500 mM D-phenylglycine methyl ester at 25°C and pH 6.5. When concentration of 6-aminopenicillic acid reaches saturation, rate of hydrolysis tends toward zero
-
-
r
D-phenylglycine methyl ester + 7-aminodeacetoxycephalosporanic acid
cephalexin + ?
-
-
-
?
D-phenylglycine methyl ester + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
cephaclor + ?
-
-
-
?
delta2-pentenylpenicillin + H2O
?
-
poor substrate
-
-
?
diethyl phenylmalonate + H2O
(+)-ethyl phenylmalonate + ethanol
-
the enantiomeric excess is higher than 98%, producing mainly the (+)-ethyl phenylmalonate ester
-
-
?
dimethyl phenylmalonate + H2O
(+)-methyl phenylmalonate + methanol
-
dimethyl phenylmalonate is fully hydrolyzed to methylphenylmalonate in only 2 h, but even after 10 h phenylmalonic acid is not detected. The enantiomeric excess is higher than 98%, producing mainly the (+)-methyl phenylmalonate ester
-
-
?
DL-alpha-hydroxybenzylpenicillin + H2O
6-aminopenicillanate + DL-alpha-hydroxyphenylacetic acid
-
-
-
?
ethyl 2-phenylacetate + H2O
2-phenylacetate + ethanol
-
-
-
-
?
ethyl 4-[(phenylacetyl)amino] benzoate + H2O
phenylacetate + 4-amino-ethylbenzoate
-
-
-
-
?
ethylthiomethylpenicillin + H2O
?
-
poor substrate
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
glutarate + 7-aminocephalosporanic acid
-
-
-
-
?
hydroxyphenylglycineamide + 7-aminodeacetoxycephalosporanic acid
cefadroxil + NH3
-
-
-
-
?
isobutoxymethylpenicillin + H2O
6-aminopenicillanate + carbonic acid isopropyl ester
-
-
-
-
?
L-phenylalanine + (2S)-2-amino-2-phenylethanamide
N-[(2S)-2-amino-2-phenylacetyl]-L-phenylalanine + NH3
-
-
-
-
?
L-phenylalanine + (2S)-2-hydroxy-2-phenylethanamide
N-[(2S)-2-hydroxy-2-phenylacetyl]-L-phenylalanine + NH3
-
-
-
-
?
L-phenylalanine + 2-(4-hydroxyphenyl)acetamide
N-[(4-hydroxyphenyl)acetyl]-L-phenylalanine + NH3
-
-
-
-
?
L-phenylalanine + methyl (2S)-hydroxy(4-hydroxyphenyl)ethanoate
N-[(2S)-2-hydroxy-2-phenylacetyl]-L-phenylalanine + methanol
-
-
-
-
?
L-phenylalanine + methyl (4-hydroxyphenyl)acetate
N-[(4-hydroxyphenyl)acetyl]-L-phenylalanine + methanol
-
-
-
-
?
L-phenylalanine + methyl phenylacetate
N-phenylacetyl-L-phenylalanine + methanol
-
-
-
-
?
L-phenylalanine + N-[(2S)-2-amino-2-phenylacetyl]-L-phenylalanine
N-[(2S)-2-amino-2-phenylacetyl]-L-phenylalanine + methanol
-
-
-
-
?
L-phenylalanine + phenylacetamide
N-phenylacetyl-L-phenylalanine + NH3
-
-
-
-
?
L-tyrosine + phenylacetamide
N-(phenylacetyl)-L-tyrosine + NH3
-
-
-
-
?
N,2-diphenylacetamide + H2O
phenylacetate + ?
-
-
-
-
?
N-(4-acetylphenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-acetylphenol
-
-
-
-
?
N-(4-bromophenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-bromophenol
-
-
-
-
?
N-(4-cyanophenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-cyanophenol
-
-
-
-
?
N-(4-methoxyphenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-methoxyphenol
-
-
-
-
?
N-(4-methylphenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-methylphenol
-
-
-
-
?
N-(4-nitrophenyl)-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-nitrophenol
-
-
-
-
?
N-(5-nitro-2-pyridyl)-phenylacetamide + H2O
phenylacetate + 5-nitropyridin-2-amine
-
-
-
-
?
N-(phenylacetyl)glycine + H2O
phenylacetic acid + glycine
-
-
-
?
N-methylphenylacetamide + H2O
?
-
poor substrate
-
-
?
N-phenylacetyl-alpha-homophenylalanine + H2O
homophenylalanine + phenylacetate
N-phenylacetyl-alpha-isoleucine + H2O
isoleucine + phenylacetate
N-phenylacetyl-alpha-leucine + H2O
leucine + phenylacetate
N-phenylacetyl-alpha-phenylalanine + H2O
phenylalanine + phenylacetate
N-phenylacetyl-alpha-tert-leucine + H2O
tert-leucine + phenylacetate
N-phenylacetyl-Asp + H2O
phenylacetic acid + Asp
-
-
-
?
N-phenylacetyl-Asp-Phe methyl ester + H2O
phenylacetic acid + Asp-Phe methyl ester
-
-
-
?
N-phenylacetyl-beta-homoleucine + H2O
beta-homoleucine + phenylacetate
N-phenylacetyl-beta-leucine + H2O
beta-leucine + phenylacetate
N-phenylacetyl-DL-leucine + H2O
?
-
-
-
-
?
N-phenylacetyl-DL-tert-leucine + H2O
L-tert-leucine + phenylacetate
-
enantioselective hydrolysis
-
-
?
N-phenylacetyl-Glu + H2O
phenylacetic acid + Glu
-
-
-
?
N-phenylacetyl-L-alpha-aminophenylacetic acid + H2O
?
-
-
-
-
?
N-phenylacetyl-Leu + H2O
phenylacetic acid + Leu
-
-
-
?
N-phenylacetyl-Phe + H2O
phenylacetic acid + Phe
-
-
-
?
N-phenylacetylglycine + H2O
?
-
-
-
-
?
n-propoxymethylpenicillin + H2O
6-aminopenicillanate + carbonic acid monopropyl ester
-
-
-
-
?
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide + H2O
phenylacetate + 2-amino-4-(methylsulfonyl)phenol
-
-
-
-
?
N2-phenylacetyl-2'-deoxyguanosine + H2O
phenylacetic acid + 2'-deoxyguanosine
N6-phenylacetyl-2'-deoxyadenosine + H2O
phenylacetic acid + 2'-deoxyadenosine
p-hydroxybenzylpenicillin + H2O
6-aminopenicillanate + p-hydroxyphenylacetic acid
-
-
-
?
penicillin + H2O
a carboxylate + 6-aminopenicillanate
-
-
-
?
penicillin dihydroF + H2O
6-aminopenicillanic acid + hexanoic acid
penicillin dihydroF + H2O
?
-
substrate of immobilized enzyme
-
-
?
penicillin F + H2O
6-aminopenicillanic acid + (3E)-hex-3-enoic acid
penicillin F + H2O
?
-
substrate of immobilized enzyme
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
penicillin G + H2O
6-aminopenicillanic acid + phenyl acetic acid
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
penicillin G + H2O
phenyl acetic acid + 6-aminopenicillanate
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanic acid
penicillin K + H2O
6-aminopenicillanic acid + octanoic acid
-
-
-
-
?
penicillin K + H2O
?
-
substrate of immobilized enzyme
-
-
?
penicillin K + H2O
octanoic acid + 6-aminopenicillanate
penicillin V + H2O
6-aminopenicillanate + phenylacetate
the conversion yields of 6-aminopenicillanate catalyzed by mutant enzyme T63S/S110C/N198Y reaches 98% with 20% (w/v) penicillin V as substrate, which is significantly higher than that of the wild-type enzyme (85%). The enhancement of specific activity of mutant enzyme is probably attributed to the changes in the number of hydrogen bonds within the molecules
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
penicillin V + H2O
a carboxylate + 6-aminopenicillanate
penicillin V + H2O
phenoxyacetate + 6-aminopenicillate
-
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
penicillin V + H2O
phenoxyacetic acid + 6-aminopenicillanate
-
-
-
-
?
penicillin V sulfoxide + H2O
?
-
-
-
-
?
penicillin-G + H2O
6-aminopenicillanic acid + phenylacetic acid
phenoxyethylpenicillin + H2O
3-phenoxypropanoic acid + 6-aminopenicillanic acid
-
poor substrate
-
-
?
phenylacetamide + 6-aminopenicillanic acid
?
phenylacetamide + H2O
NH3 + ?
-
poor substrate
-
-
?
phenylacetamide + H2O
phenylacetate + NH3
-
-
-
-
?
phenylacetate 4-nitroanilide + H2O
phenylacetic acid + 4-nitrolaniline
-
-
-
-
?
phenylacetic acid + 6-aminopenicillanic acid
penicillin G
phenylacetic hydrazide + H2O
phenylacetate + hydrazine
-
-
-
-
?
phenylacetyl-2-naphthylamide + H2O
phenylacetate + 2-naphthylamine
-
-
-
-
?
phenylacetyl-4-aminobenzoic acid
4-aminobenzoic acid + phenylacetic acid
-
colometric assay
-
?
phenylacetyl-7-amido-4-methylcoumarin + H2O
phenylacetate + 7-amino-4-methylcoumarin
-
-
-
-
?
phenylacetyl-Gly + H2O
phenylacetic acid + glycine
-
-
-
?
phenylacetyl-L-asparagine + H2O
L-asparagine + phenylacetic acid
-
-
-
?
phenylacetyl-L-leucine + H2O
?
-
-
-
-
?
phenylacetylanthranilic acid + H2O
phenylacetate + 2-aminobenzoic acid
-
colometric assay
-
?
phenylacetylaspartame + H2O
phenylacetic acid + aspartame
-
-
-
?
phenylacetylglycine + 6-aminopenicillanic acid
penicillin G + glycine
phenylglycine methyl ester + aminopenicillanic acid
ampicillin + methanol
-
-
-
?
phenylglycineamide + 7-aminodeacetoxycephalosporanic acid
cefaclor + NH3
-
-
-
-
?
phenylglycinemethylester + 7-aminodeacetoxymethyl-3-chlorocephalosporanic acid
cephalexin + methanol
-
-
-
-
?
vanillylamine + methyl laurate
6-lauroyl-vanillylamide + methanol
-
-
-
-
?
vanillylamine + methyl myristate
6-myristoyl-vinillylamide + methanol
-
-
-
-
?
vanillylamine + methyl n-octanoate
6-octanoyl-vanillylamide + methanol
-
-
-
-
?
[(2R,3S),(2S,3R)]-cis-3-amino-azetidinone + phenoxy-acetic acid methyl ester
(2R,3S)-beta-lactam intermediate + methanol
-
-
-
-
?
additional information
?
-
(R)-2-phenylglycine amide + H2O

(R)-2-phenylglycine + hydroxylamine
-
-
-
?
(R)-2-phenylglycine amide + H2O
(R)-2-phenylglycine + hydroxylamine
-
-
-
?
(R)-2-phenylglycine amide + H2O
(R)-2-phenylglycine + hydroxylamine
-
-
-
?
(R)-2-phenylglycine amide + H2O
(R)-2-phenylglycine + hydroxylamine
-
-
-
?
(R)-2-phenylglycine methyl ester + H2O

(R)-2-phenylglycine + methanol
-
-
-
?
(R)-2-phenylglycine methyl ester + H2O
(R)-2-phenylglycine + methanol
-
-
-
?
(R)-2-phenylglycine methyl ester + H2O
(R)-2-phenylglycine + methanol
-
-
-
?
(R)-2-phenylglycine methyl ester + H2O
(R)-2-phenylglycine + methanol
-
-
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O

5-amino-2-nitrobenzoic acid + phenoxyacetate
-
-
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
5-amino-2-nitrobenzoic acid + phenoxyacetate
-
-
-
?
2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O
5-amino-2-nitrobenzoic acid + phenoxyacetate
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O

phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O

phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
?
2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
?
3-nitro-6-phenylacetaminobenzoic acid + H2O

?
-
-
-
-
?
3-nitro-6-phenylacetaminobenzoic acid + H2O
?
-
-
-
-
?
4-hydroxyphenylacetamide + 6-aminopenicillanic acid

?
-
-
-
r
4-hydroxyphenylacetamide + 6-aminopenicillanic acid
?
-
-
-
r
6-aminopenicillanate + phenylacetic acid

penicillin G + H2O
-
-
-
r
6-aminopenicillanate + phenylacetic acid
penicillin G + H2O
synthesis of penicillin G by enzyme is significantly better than by Escherichia coli enzyme
-
-
r
6-nitro-3-(phenylacetamido)-benzoic acid + H2O

3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetate
-
-
-
?
6-nitro-3-(phenylacetamido)benzoic acid + H2O

?
-
-
-
-
?
6-nitro-3-(phenylacetamido)benzoic acid + H2O
?
-
-
-
?
6-nitro-3-phenylacetamide benzoic acid + H2O

3-amino-6-nitrobenzoic acid + phenylacetic acid
-
-
-
?
6-nitro-3-phenylacetamide benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
-
-
-
-
?
6-nitro-3-phenylacetamido-benzoic acid + H2O

?
-
-
-
?
6-nitro-3-phenylacetamido-benzoic acid + H2O
?
-
-
-
?
6-nitro-3-phenylacetamidobenzoic acid + H2O

?
-
-
-
?
6-nitro-3-phenylacetamidobenzoic acid + H2O
?
-
-
-
?
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O

3-amino-6-nitrobenzoic acid + phenylacetic acid
-
-
-
?
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
-
-
-
-
?
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
-
-
-
r
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
3-amino-6-nitrobenzoic acid + phenylacetic acid
-
-
-
r
amoxicillin + H2O

D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
-
-
-
r
amoxicillin + H2O
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
-
-
?
amoxicillin + H2O
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
-
-
?
amoxillin + H2O

D-4-hydroxyphenylglycine + 6-aminopenicillanic acid
-
-
-
r
amoxillin + H2O
D-4-hydroxyphenylglycine + 6-aminopenicillanic acid
-
-
-
r
ampicillin + H2O

(R)-2-phenylglycine + 6-aminopenicillanic acid
-
-
-
-
?
ampicillin + H2O
(R)-2-phenylglycine + 6-aminopenicillanic acid
-
-
-
?
ampicillin + H2O
(R)-2-phenylglycine + 6-aminopenicillanic acid
-
-
-
-
r
ampicillin + H2O
(R)-2-phenylglycine + 6-aminopenicillanic acid
-
-
-
r
ampicillin + H2O
(R)-2-phenylglycine + 6-aminopenicillanic acid
-
-
-
r
ampicillin + H2O

6-aminopenicillanic acid + (R)-2-phenylglycine
-
-
-
-
?
ampicillin + H2O
6-aminopenicillanic acid + (R)-2-phenylglycine
Pseudomonas melanogenum
-
-
-
r
ampicillin + H2O
6-aminopenicillanic acid + (R)-2-phenylglycine
-
-
-
?
ampicillin + H2O

?
-
-
-
?
ampicillin + H2O
?
-
-
-
?
benzylpenicillin + H2O

benzoate + 6-aminopenicillanate
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
-
-
-
?
benzylpenicillin + H2O
benzoate + 6-aminopenicillanate
-
higher activity of enzyme immobilised on the amino support suggests that interactions occurring between the amino carrier and the glycan favours a more correct orientation of the protein making it accessible to substrates
-
-
?
benzylpenicillin + H2O

phenylacetic acid + 6-aminopenicillanate
-
-
-
-
?
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
-
highly specific
-
-
?
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
-
complete hydrolysis at low substrate concentrations
-
-
?
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
-
highly specific
-
-
?
benzylpenicillin + H2O
phenylacetic acid + 6-aminopenicillanate
-
complete hydrolysis at low substrate concentrations
-
-
?
cefadroxil + H2O

?
-
-
-
r
cefadroxil + H2O
?
-
-
-
r
cephalexin + H2O

7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine
Pseudomonas melanogenum
-
-
-
-
?
cephalexin + H2O
7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine
-
-
-
?
cephalexin + H2O

7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
-
-
-
?
cephalexin + H2O
7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
-
-
-
?
cephalexin + H2O
7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
-
-
r
cephalexin + H2O
7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
-
-
r
cephaloridine + H2O

(6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
-
-
-
-
?
cephaloridine + H2O
(6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
-
-
-
-
?
cephalosporin + H2O

7-aminocephalosporanic acid + an amino acid
-
-
-
?
cephalosporin + H2O
7-aminocephalosporanic acid + an amino acid
-
-
-
-
?
cephalothin + H2O

(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
-
-
-
-
?
cephalothin + H2O
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
-
-
-
-
?
cephalothin + H2O
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
-
-
-
-
?
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O

phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
r
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
-
-
r
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid

amoxicillin + NH3
-
-
-
-
r
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
amoxicillin + NH3
-
-
-
r
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
amoxicillin + NH3
-
-
-
r
D-hydroxyphenylglycine methyl ester + H2O

?
-
-
-
?
D-hydroxyphenylglycine methyl ester + H2O
?
-
-
-
?
D-phenylglycine amide + 6-aminopenicillanic acid

ampicillin + H2O
-
-
-
-
r
D-phenylglycine amide + 6-aminopenicillanic acid
ampicillin + H2O
-
-
-
r
D-phenylglycine amide + 6-aminopenicillanic acid
ampicillin + H2O
-
-
-
r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid

cephalexin
-
kinetically controlled synthesis
-
-
r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin
-
kinetically controlled synthesis
-
-
r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin
-
kinetically controlled synthesis
-
-
r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid
cephalexin
-
kinetically controlled synthesis
-
-
r
N-phenylacetyl-alpha-homophenylalanine + H2O

homophenylalanine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80
-
-
?
N-phenylacetyl-alpha-homophenylalanine + H2O
homophenylalanine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80
-
-
?
N-phenylacetyl-alpha-isoleucine + H2O

isoleucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80
-
-
?
N-phenylacetyl-alpha-isoleucine + H2O
isoleucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80
-
-
?
N-phenylacetyl-alpha-leucine + H2O

leucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80
-
-
?
N-phenylacetyl-alpha-leucine + H2O
leucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 80
-
-
?
N-phenylacetyl-alpha-phenylalanine + H2O

phenylalanine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 90
-
-
?
N-phenylacetyl-alpha-phenylalanine + H2O
phenylalanine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 90
-
-
?
N-phenylacetyl-alpha-tert-leucine + H2O

tert-leucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 105
-
-
?
N-phenylacetyl-alpha-tert-leucine + H2O
tert-leucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 75
-
-
?
N-phenylacetyl-beta-homoleucine + H2O

beta-homoleucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 85
-
-
?
N-phenylacetyl-beta-homoleucine + H2O
beta-homoleucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 55
-
-
?
N-phenylacetyl-beta-leucine + H2O

beta-leucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 68
-
-
?
N-phenylacetyl-beta-leucine + H2O
beta-leucine + phenylacetate
experimentally determined E-value (enantioselectivity) towards racemic mixtures of alpha- and beta-amino acids: 60
-
-
?
N2-phenylacetyl-2'-deoxyguanosine + H2O

phenylacetic acid + 2'-deoxyguanosine
-
-
-
?
N2-phenylacetyl-2'-deoxyguanosine + H2O
phenylacetic acid + 2'-deoxyguanosine
-
-
-
?
N6-phenylacetyl-2'-deoxyadenosine + H2O

phenylacetic acid + 2'-deoxyadenosine
-
-
-
?
N6-phenylacetyl-2'-deoxyadenosine + H2O
phenylacetic acid + 2'-deoxyadenosine
-
-
-
?
penicillin dihydroF + H2O

6-aminopenicillanic acid + hexanoic acid
-
-
-
-
?
penicillin dihydroF + H2O
6-aminopenicillanic acid + hexanoic acid
-
-
-
?
penicillin dihydroF + H2O
6-aminopenicillanic acid + hexanoic acid
-
-
-
?
penicillin F + H2O

6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
-
-
-
?
penicillin F + H2O
6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
-
-
?
penicillin F + H2O
6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
-
-
?
penicillin G + H2O

6-aminopenicillanate + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
a tryptophan residue is important for binding substrate penicillin G
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanate + phenylacetic acid
-
-
-
r
penicillin G + H2O

6-aminopenicillanic acid + phenyl acetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenyl acetic acid
penicillin G acylase is a type II penicillin acylase
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenyl acetic acid
-
-
-
-
?
penicillin G + H2O

6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
r
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
r
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
r
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
enzyme specific for penicillin G
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
function in nature not fully understood, possibly functions to degrade phenylacetylated compounds in order to generate phenylacetic acid which can be used as carbon source
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
r
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
?
penicillin G + H2O

phenyl acetic acid + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenyl acetic acid + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O

phenylacetate + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
penicillin G acylases preferentially hydrolyze penicillin G, i.e. benzylpenicillin
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O
phenylacetate + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O

phenylacetic acid + 6-aminopenicillanate
-
-
product detection by reaction with substrate 6-nitro-3-(phenylacetamido)benzoic acid
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
detection of product 6-aminopenicillanate by reaction with p-dimethylaminobenzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
product detection by p-dimethylamine benzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
product detection by p-dimethylamine benzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
detection of product 6-aminopenicillanate by reaction with p-dimethylaminobenzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
hydrolysis of penicillin G to obtain 6-aminopenicillanate in a batch reactor
detection of product 6-aminopenicillanate by reaction with p-dimethylaminobenzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
hydrolysis of penicillin G to obtain 6-aminopenicillanate in a batch reactor
detection of product 6-aminopenicillanate by reaction with p-dimethylaminobenzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
detection by reaction with p-dimethyl-aminobenzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
product detection by p-dimethylamine benzaldehyde
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
catalysis of the reverse reaction at low pH or low water activity
-
-
r
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanate
-
-
product detection by p-dimethylamine benzaldehyde
-
?
penicillin G + H2O

phenylacetic acid + 6-aminopenicillanic acid
-
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanic acid
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanic acid
-
-
-
?
penicillin G + H2O
phenylacetic acid + 6-aminopenicillanic acid
-
-
-
?
penicillin K + H2O

octanoic acid + 6-aminopenicillanate
-
-
-
?
penicillin K + H2O
octanoic acid + 6-aminopenicillanate
-
-
-
?
penicillin K + H2O
octanoic acid + 6-aminopenicillanate
-
-
-
?
penicillin K + H2O
octanoic acid + 6-aminopenicillanate
preferred substrate for the wild-type enzyme
-
-
?
penicillin V + H2O

6-aminopenicillanic acid + phenoxyacetic acid
-
the enzyme is highly specific towards penicillin V
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
penicillin G: negligible activity, ampicillin or amoxicillin: no activity
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
poor substrate
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
poor substrate
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
substrate of soluble and immobilized enzyme
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
-
?
penicillin V + H2O
6-aminopenicillanic acid + phenoxyacetic acid
-
-
-
-
?
penicillin V + H2O

a carboxylate + 6-aminopenicillanate
-
-
-
-
?
penicillin V + H2O
a carboxylate + 6-aminopenicillanate
-
involvement of serine and tryptophan in the enzyme activity
-
-
?
penicillin V + H2O

phenoxyacetate + 6-aminopenicillinate
-
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
-
specific for penicillin V
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
-
-
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
-
-
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
-
-
-
?
penicillin V + H2O
phenoxyacetate + 6-aminopenicillinate
-
-
-
?
penicillin-G + H2O

6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
penicillin-G + H2O
6-aminopenicillanic acid + phenylacetic acid
-
-
-
-
?
phenylacetamide + 6-aminopenicillanic acid

?
-
-
-
r
phenylacetamide + 6-aminopenicillanic acid
?
-
-
-
r
phenylacetamide + H2O

?
-
-
-
-
?
phenylacetamide + H2O
?
-
-
-
-
?
phenylacetamide + H2O
?
-
poor substrate
-
-
?
phenylacetamide + H2O
?
-
poor substrate
-
-
?
phenylacetic acid + 6-aminopenicillanic acid

penicillin G
-
-
-
r
phenylacetic acid + 6-aminopenicillanic acid
penicillin G
-
-
-
r
phenylacetylglycine + 6-aminopenicillanic acid

penicillin G + glycine
-
-
-
r
phenylacetylglycine + 6-aminopenicillanic acid
penicillin G + glycine
-
-
-
r
additional information

?
-
-
no activity with cefaclor, cephalexin, ampicillin, dicloxacillin or cephalosporin G
-
-
?
additional information
?
-
-
substrate specificity with phenylacetyl p-substituted anilides, overview
-
-
?
additional information
?
-
-
no activity in hydrolysis of conjugated bile salts
-
-
?
additional information
?
-
substrate specificity, overview. No activity with ampicillin, amoxicillin, or cephalosporin. Penicillin G is a poor substrate
-
-
?
additional information
?
-
-
substrate specificity, overview. No activity with ampicillin, amoxicillin, or cephalosporin. Penicillin G is a poor substrate
-
-
?
additional information
?
-
-
overview on best substrates for similar organisms
-
-
?
additional information
?
-
-
intracellular proteolysis in the cytoplasm is one of the important steps that limit the yield of active penicillin amidase from the translational product pre-pro-peniciliin amidase. Glucose and temperature are important parameters, determining the degradation rate of the proteolytically sensitive pre-pro-penicillin amidase
-
?
additional information
?
-
-
penicillin acylase is able to both hydrolyze and synthesize beta-lactam antibiotics
-
-
?
additional information
?
-
-
other natural penicillins
-
-
?
additional information
?
-
-
overview on best substrates for similar organisms
-
-
?
additional information
?
-
-
autoproteolytic processing of the alpha and beta subunit
-
-
?
additional information
?
-
-
no activity with cephapirin, cefotaximen cephalosporin, and penicillin N
-
-
?
additional information
?
-
-
no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin
-
-
?
additional information
?
-
-
no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin
-
-
?
additional information
?
-
Pseudomonas melanogenum
-
other natural penicillins
-
-
?
additional information
?
-
the acylase shows a marked preference for penicillin K over penicillin G, substrate specificity, overview
-
-
?
additional information
?
-
-
the acylase shows a marked preference for penicillin K over penicillin G, substrate specificity, overview
-
-
?
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2.5
2-benzoxazolon-3-yl-acetic acid methyl ester
-
pH 6.8, 25°C
0.63
2-nitro-5-(phenoxyacetamido)-benzoic acid
pH 6.6, 40°C
1.6
2-nitro-5-phenoxyacetamide benzoic acid
-
0.081
2-nitro-5-phenylacetamidobenzoic acid
-
pH 7.5, 25°C
0.00614 - 0.03
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
0.0005 - 30.39
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
0.147
2-phenyl-N-(4-sulfamoylphenyl)acetamide
-
pH 7.0, 25°C
0.00063
2-phenylacetamidobenzoic acid
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.0004
3-phenylacetamidobenzoic acid
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.027 - 0.114
4-Hydroxyphenylacetamide
0.031
4-nitrophenyl acetate
-
-
0.0003
4-phenylacetamidobenzoic acid
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.07 - 0.23
5-nitro-3-[(phenylacetyl)amino]benzoic acid
0.0083 - 0.0197
6-nitro-3-(phenylacetamido)-benzoic acid
0.0176
6-nitro-3-phenylacetamide benzoic acid
-
pH 7.5, 37°C
0.012 - 0.02
6-nitro-3-phenylacetamidobenzoic acid
0.000015 - 0.027
6-nitro-3-[(phenylacetyl)amino]benzoic acid
0.01
7-phenylacetamidodeacetoxycephalosporanic acid
-
-
0.399 - 41.26
amoxicillin
0.105
amoxycillin
pH 7.4, 37°C, recombinant enzyme
0.0031 - 4.5
benzylpenicillin
2
benzylpenicilloic acid
-
-
0.0519
carbenicillin
pH 7.4, 37°C, recombinant enzyme
1.79
cephadrine
Pseudomonas melanogenum
-
-
1.96
cephaloglycin
Pseudomonas melanogenum
-
-
0.0164 - 0.1
cephaloridine
0.0156 - 0.042
cephalothin
3.2
D-(-)-alpha-aminophenylacetic acid p-nitroanilide
-
-
0.646 - 1.3
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid
9.1 - 12.2
D-4-hydroxyphenylglycine amide
1.6723 - 51.15
D-hydroxyphenylglycine methyl ester
-
2 - 30
D-phenylglycine amide
0.045
ethyl 2-phenylacetate
-
-
-
0.153
ethyl 4-[(phenylacetyl)amino] benzoate
-
pH 7.0, 25°C
0.137
N,2-diphenylacetamide
-
pH 7.0, 25°C
0.23
N-(4-acetylphenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.171
N-(4-bromophenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.16
N-(4-cyanophenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.162
N-(4-methoxyphenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.121
N-(4-methylphenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.095
N-(4-nitrophenyl)-2-phenylacetamide
-
pH 7.0, 25°C
0.00051
N-(5-nitro-2-pyridyl)-phenylacetamide
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.159
N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide
-
pH 7.0, 25°C
0.8
N2-phenylacetyl-2'-deoxyguanosine
-
pH 7.8, 25°C
0.46
N6-phenylacetyl-2'-deoxyadenosine
-
pH 7.8, 25°C
0.82 - 5.6
penicillin dihydroF
0.00001 - 164
penicillin G
0.0002 - 0.156
phenylacetamide
0.0004
phenylacetate 4-nitroanilide
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.097
phenylacetate p-nitroanilide
-
-
0.0002
phenylacetyl-2-naphthylamide
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.4
phenylacetyl-3-aminobenzoic acid
-
-
0.18
phenylacetyl-4-aminobenzoic acid
-
-
0.000012
phenylacetyl-7-amido-4-methylcoumarin
-
25°C, pH 7.0, 10% dimethylsulfoxide
0.95
phenylacetyl-anthranilic acid
-
-
5.2
phenylacetyl-Gly
-
pH 7.8, 25°C
0.67
phenylacetylaspartame
-
pH 7.8, 25°C
0.08
phenylacetylglycine
-
-
additional information
additional information
-
0.00614
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid

-
pH 7.0, 25°C, mutant N241G
0.0102
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
-
pH 7.0, 25°C, mutant N241S
0.03
2-nitro-5-[(phenyl-acetyl)amino]benzoic acid
-
pH 7.0, 25°C
0.0005
2-nitro-5-[(phenylacetyl)amino]-benzoic acid

alphaF146Y mutant protein, pH 7.5, 25°C
0.015
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
pH 7.0, 30°C
0.026
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
wild type protein, pH 7.5, 25°C
0.098
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
betaF24A mutant protein, pH 7.5, 25°C
0.116
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
alphaF146A mutant protein, pH 7.5, 25°C
0.272
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
alphaF146Q mutant protein, pH 7.5, 25°C
0.386
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
alphaF146R mutant protein, pH 7.5, 25°C
0.551
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
betaF24S mutant protein, pH 7.5, 25°C
0.797
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
betaF24P mutant protein, pH 7.5, 25°C
0.9919
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
pH 7.0, 30°C, soluble enzyme
7.9
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates
30.39
2-nitro-5-[(phenylacetyl)amino]-benzoic acid
pH 7.0, 30°C, cross-linked enzyme aggregates
0.027
4-Hydroxyphenylacetamide

pH 7.0, 30°C
0.114
4-Hydroxyphenylacetamide
pH 7.0, 30°C
0.07
5-nitro-3-[(phenylacetyl)amino]benzoic acid

-
pH 7.5, 25°C, native enzyme
0.14
5-nitro-3-[(phenylacetyl)amino]benzoic acid
-
pH 7.5, 25°C, fresh immobilized cross-linked enzyme aggregates
0.23
5-nitro-3-[(phenylacetyl)amino]benzoic acid
-
pH 7.5, 25°C, mature immobilized cross-linked enzyme aggregates
0.0083
6-nitro-3-(phenylacetamido)-benzoic acid

pH 7.4, 37°C, recombinant enzyme
0.0197
6-nitro-3-(phenylacetamido)-benzoic acid
-
pH and temperature not specified in the publication
0.012
6-nitro-3-phenylacetamidobenzoic acid

-
pH 7.5, 25°C, enzyme form PA5.3
0.017
6-nitro-3-phenylacetamidobenzoic acid
-
pH 7.5, 25°C, enzyme form PA5.5
0.02
6-nitro-3-phenylacetamidobenzoic acid
-
pH 7.5, 25°C
0.000015
6-nitro-3-[(phenylacetyl)amino]benzoic acid

-
25°C, pH 7.0, 10% dimethylsulfoxide
0.004
6-nitro-3-[(phenylacetyl)amino]benzoic acid
pH 7.0, 30°C
0.027
6-nitro-3-[(phenylacetyl)amino]benzoic acid
pH 8.0, 37°C
0.399
amoxicillin

pH 7.0, 30°C
1.007
amoxicillin
pH 7.0, 30°C, soluble enzyme
1.07
amoxicillin
pH 7.0, 30°C
7.31
amoxicillin
pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates
41.26
amoxicillin
pH 7.0, 30°C, cross-linked enzyme aggregates
0.0944
ampicillin

pH 7.4, 37°C, recombinant enzyme
0.575
ampicillin
pH 7.0, 30°C
1.9
ampicillin
-
mutant alphaR145L
2.5
ampicillin
pH 7.0, 30°C
3.6
ampicillin
-
wild-type enzyme
4.34
ampicillin
Pseudomonas melanogenum
-
-
4.6
ampicillin
-
mutant alphaR145S
5.2
ampicillin
-
mutant alphaR145G
0.0031
benzylpenicillin

-
-
0.0046
benzylpenicillin
-
-
0.005
benzylpenicillin
-
pH 7.5, 25°C
0.0065
benzylpenicillin
-
pH 7.5, 25°C, mutant enzyme T206G/S213G/T219G
0.007
benzylpenicillin
-
pH 7.5, 25°C, mutant enzyme T206G//S213G
0.008
benzylpenicillin
-
pH 7.5, 25°C, enzyme form PA5..5 and PA5.3
0.008
benzylpenicillin
-
pH 7.5, 25°C, mutant enzyme T206G and wild-type enzyme
0.01
benzylpenicillin
-
pH 7.5, 25°C
0.016
benzylpenicillin
-
pH 7.5, 40°C
0.018
benzylpenicillin
-
pH 7.5, 25°C
0.061
benzylpenicillin
-
pH 7.5, 40°C, presence of 50 mM 2,3-butanedione
0.065
benzylpenicillin
-
pH 7.5, 40°C, presence of 10 mM phenylglyoxal
0.67
benzylpenicillin
-
-
0.8
benzylpenicillin
-
immobilized enzyme
4.5
benzylpenicillin
-
37°C, pH 8.7
0.284
cefadroxil

pH 7.0, 30°C
0.646
cefadroxil
pH 7.0, 30°C
0.0255
cephalexin

pH 7.4, 37°C, recombinant enzyme
1.3
cephalexin
pH 7.0, 30°C
1.5
cephalexin
pH 7.0, 30°C
1.61
cephalexin
Pseudomonas melanogenum
-
-
0.0164
cephaloridine

pH 7.4, 37°C, recombinant enzyme
0.0156
cephalothin

pH 7.4, 37°C, recombinant enzyme
0.646
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid

pH 7.0, 30°C
1.3
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid
pH 7.0, 30°C
9.1
D-4-hydroxyphenylglycine amide

pH 7.0, 30°C
12.2
D-4-hydroxyphenylglycine amide
pH 7.0, 30°C
1.6723
D-hydroxyphenylglycine methyl ester

pH 7.0, 30°C, soluble enzyme
-
11.74
D-hydroxyphenylglycine methyl ester
pH 7.0, 30°C, poly-lysine supported cross-linked enzyme aggregates
-
51.15
D-hydroxyphenylglycine methyl ester
pH 7.0, 30°C, cross-linked enzyme aggregates
-
2 - 3
D-phenylglycine amide

-
mutant alphaR145G
9.1
D-phenylglycine amide
-
mutant alphaR145L
12
D-phenylglycine amide
pH 7.0, 30°C
13
D-phenylglycine amide
-
mutant alphaR145S
27
D-phenylglycine amide
-
wild-type enzyme
30
D-phenylglycine amide
pH 7.0, 30°C
0.82
penicillin dihydroF

pH 8.0, 40°C recombinant enzyme
5.6
penicillin dihydroF
-
pH 8.0, 45°C
1.39
penicillin F

pH 8.0, 40°C recombinant enzyme
15.1
penicillin F
-
pH 8.0, 45°C
0.00001
penicillin G

-
25°C, pH 7.0, 10% dimethylsulfoxide
0.0046
penicillin G
pH 7.4, 37°C, recombinant enzyme
0.0089
penicillin G
pH 8.0, 37°C
0.012
penicillin G
pH 7.0, 30°C
0.013
penicillin G
pH 7.0, 30°C
0.016
penicillin G
-
unmodified enzyme
0.051
penicillin G
-
enzyme modified with 2-hydroxy 5-nitrobenzylbromide
0.054
penicillin G
-
enzyme modified with N-bromosuccinamide
0.42
penicillin G
pH and temperature not specified in the publication
1.83
penicillin G
pH and temperature not specified in the publication
2.7
penicillin G
pH 7.5, 50°C
3
penicillin G
-
pH 8.0, 40°C
4.22
penicillin G
-
immobilized enzyme
13.4
penicillin G
pH 5.5, 65°C, recombinant mutant L24F
15.5
penicillin G
pH 5.5, 65°C, recombinant mutant L188F
17.8
penicillin G
pH 5.5, 65°C, recombinant mutant S189F
19
penicillin G
-
pH 7.8, 25°C
19.5
penicillin G
-
pH 7.0, 40°C, free enzyme
36.5
penicillin G
pH 5.5, 65°C, recombinant mutant I57F
37.6
penicillin G
pH 5.5, 65°C, recombinant mutant L24F/I57F
43.3
penicillin G
pH 5.5, 65°C, recombinant mutant L188F/S189F
44.4
penicillin G
pH 5.5, 65°C, recombinant mutant L188F/L24F/I57F
47.4
penicillin G
-
pH 7.0, 40°C, immobilized enzyme
56.19
penicillin G
-
soluble enzyme, pH and temperature not specified in the publication
58.18
penicillin G
pH 8.0, 40°C recombinant enzyme
64.84
penicillin G
-
enzyme immobilized on alginate beads, pH and temperature not specified in the publication
87.08
penicillin G
-
enzyme immobilized on alginate/chitosan hybrid beads, pH and temperature not specified in the publication
155.8
penicillin G
-
pH 8.0, 45°C
164
penicillin G
pH 5.5, 65°C, recombinant wild-type enzyme
0.05
penicillin K

pH 5.5, 65°C, recombinant mutant L24F/I57F
0.1
penicillin K
pH 5.5, 65°C, recombinant mutants L188F and S189F
0.12
penicillin K
pH 5.5, 65°C, recombinant mutant L24F
0.14
penicillin K
pH 8.0, 40°C recombinant enzyme
0.15
penicillin K
pH 5.5, 65°C, recombinant mutant L188F/L24F/I57F
0.27
penicillin K
pH 5.5, 65°C, recombinant mutant I57F
0.32
penicillin K
pH 5.5, 65°C, recombinant wild-type enzyme
1
penicillin K
-
pH 8.0, 45°C
0.0393
Penicillin V

pH 7.4, 37°C, recombinant enzyme
3.68
Penicillin V
pH 8.0, 40°C recombinant enzyme
15.4
Penicillin V
-
pH 8.0, 45°C
17.1
Penicillin V
-
immobilized enzyme
21.95
Penicillin V
pH 6.0, 37°C, wild-type enzyme
26.39
Penicillin V
pH 6.0, 37°C, mutant enzyme T63S/S110C/N198Y
40
Penicillin V
pH 6.6, 40°C
0.0002
phenylacetamide

-
25°C, pH 7.0, 10% dimethylsulfoxide
0.03
phenylacetamide
pH 7.0, 30°C
0.156
phenylacetamide
pH 7.0, 30°C
additional information
additional information

-
further values of mutants
-
additional information
additional information
-
temperature dependence of Km-value for (R)- and (S)p-hydroxyphenylglycineamide
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
kinetics, overview
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
MichaelisâMenten kinetics
-
additional information
additional information
-
MichaelisâMenten kinetics
-
additional information
additional information
-
kinetic results on the hydrolysis of a series of 4-substituted anilides catalyzed by penicillin G acylase from Alcaligenes faecalis
-
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