Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HB101, sequence shows one mutation resulting in a betaVal148Leu substitution in the mature protein as compared with the Swiss-Prot entry P06875 derived from Escherichia coli ATC11105 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
alphaF146A | mutant selective for (S)-ampicillin synthesis | Escherichia coli |
alphaF146Q | mutant selective for (S)-ampicillin synthesis | Escherichia coli |
alphaF146R | mutant selective for (S)-ampicillin synthesis | Escherichia coli |
alphaF146Y | mutant selective for (S)-ampicillin synthesis | Escherichia coli |
betaF24A | mutant selective for (R)-ampicillin synthesis | Escherichia coli |
betaF24C | mutant selective for (R)-ampicillin synthesis | Escherichia coli |
betaF24P | mutant selective for (R)-ampicillin synthesis | Escherichia coli |
betaF24S | mutant selective for (R)-ampicillin synthesis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | alphaF146Y mutant protein, pH 7.5, 25°C | Escherichia coli | |
0.026 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | wild type protein, pH 7.5, 25°C | Escherichia coli | |
0.098 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | betaF24A mutant protein, pH 7.5, 25°C | Escherichia coli | |
0.116 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | alphaF146A mutant protein, pH 7.5, 25°C | Escherichia coli | |
0.272 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | alphaF146Q mutant protein, pH 7.5, 25°C | Escherichia coli | |
0.386 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | alphaF146R mutant protein, pH 7.5, 25°C | Escherichia coli | |
0.551 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | betaF24S mutant protein, pH 7.5, 25°C | Escherichia coli | |
0.797 | - |
2-nitro-5-[(phenylacetyl)amino]-benzoic acid | betaF24P mutant protein, pH 7.5, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P06875 | - |
- |
Purification (Comment) | Organism |
---|---|
periplasmic fraction | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.049 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, alphaF146Q mutant protein, pH 7.5, 25°C | Escherichia coli |
0.053 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, alphaF146R mutant protein, pH 7.5, 25°C | Escherichia coli |
0.073 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, alphaF146A mutant protein, pH 7.5, 25°C | Escherichia coli |
0.6 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, betaF24S mutant protein, pH 7.5, 25°C | Escherichia coli |
1 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, betaF24A mutant protein, pH 7.5, 25°C | Escherichia coli |
1.3 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, alphaF146Y mutant protein, pH 7.5, 25°C | Escherichia coli |
2.9 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, betaF24P mutant protein, pH 7.5, 25°C | Escherichia coli |
17.3 | - |
substrate 2-nitro-5-[(phenylacetyl)amino]-benzoic acid, wild type protein, pH 7.5, 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitro-5-[(phenylacetyl)amino]-benzoic acid + H2O | - |
Escherichia coli | 5-amino-2-nitrobenzoic acid + ? | - |
? | |
phenylglycine methyl ester + aminopenicillanic acid | - |
Escherichia coli | ampicillin + methanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
penicillin G acylase | - |
Escherichia coli |