Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the recombinant enzyme by covalent linking to epoxy-activated beads in homogenous distribution to avoid enzyme aggregate formation, and usage as biocatalyst. The immobilized enzyme shows altered optimal reaction conditions and enlarged substrate specificity, as well as increased thermal stability compared to the soluble enzyme, it can be recycled 30times in consecutive batch reactions, overview | Streptomyces lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin dihydroF + H2O | Streptomyces lavendulae | substrate of immobilized enzyme | ? | - |
? | |
penicillin F + H2O | Streptomyces lavendulae | substrate of immobilized enzyme | ? | - |
? | |
penicillin K + H2O | Streptomyces lavendulae | substrate of immobilized enzyme | ? | - |
? | |
penicillin V + H2O | Streptomyces lavendulae | substrate of soluble and immobilized enzyme | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin dihydroF + H2O | substrate of immobilized enzyme | Streptomyces lavendulae | ? | - |
? | |
penicillin F + H2O | substrate of immobilized enzyme | Streptomyces lavendulae | ? | - |
? | |
penicillin K + H2O | substrate of immobilized enzyme | Streptomyces lavendulae | ? | - |
? | |
penicillin V + H2O | substrate of soluble and immobilized enzyme | Streptomyces lavendulae | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
soluble recombinant enzyme | Streptomyces lavendulae |
65 | - |
immobilized recombinant enzyme | Streptomyces lavendulae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 8.0, half-life of the soluble enzyme is 397 h, and for the immobilized enzyme 625 h | Streptomyces lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
soluble recombinant enzyme | Streptomyces lavendulae |
8.5 | - |
immobilized recombinant enzyme | Streptomyces lavendulae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
40°C, half-life of the soluble enzyme is 397 h, and for the immobilized enzyme 625 h | Streptomyces lavendulae |