Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxy 5-nitrobenzylbromide | - |
Kluyvera cryocrescens | |
N-bromosuccinamide | 0.1 mM, 70% loss of activity | Kluyvera cryocrescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
penicillin G | unmodified enzyme | Kluyvera cryocrescens | |
0.051 | - |
penicillin G | enzyme modified with 2-hydroxy 5-nitrobenzylbromide | Kluyvera cryocrescens | |
0.054 | - |
penicillin G | enzyme modified with N-bromosuccinamide | Kluyvera cryocrescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyvera cryocrescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyvera cryocrescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylpenicillin + H2O | - |
Kluyvera cryocrescens | phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | a tryptophan residue is important for binding substrate penicillin G | Kluyvera cryocrescens | 6-aminopenicillanate + phenylacetic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KcPGA | - |
Kluyvera cryocrescens |
penicillin G acylase | - |
Kluyvera cryocrescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
63 | - |
penicillin G | unmodified enzyme, enzyme modified with N-bromosuccinamide or enzyme modified with 2-hydroxy 5-nitrobenzylbromide | Kluyvera cryocrescens |