Cloned (Comment) | Organism |
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a penicillin G acylase gene from Alcaligenes faecalis and a pcm gene encoding protein isoaspartate methyltransferase (PIMT) are constructed into pET43.1a(+) and pET28a(+), respectively. The recombinant plasmids pETAFPGA and pETPCM are transformed into the same host cell Escherichia coli BL21 (DE3). The two plasmids can exist in the host cell and the two genes can be efficiently expressed after induction. The product of pcm gene can function as a helper molecule for penicillin G acylase. PIMT increases the enzymatic activities in supernatant of ferment broth (1.6folds) and cell lysate (1.8folds), while it does not significantly affect the expression level of penicillin G acylase | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
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Alcaligenes faecalis | - |
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Synonyms | Comment | Organism |
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penicillin G acylase | - |
Alcaligenes faecalis |