Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chymostatin | 0.05 mg/ml, activation to 170% of the original activity, peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide | Candida albicans | |
leupeptin | 0.05 mg/ml, activation to 125% of the original activity, peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide | Candida albicans | |
polylysine | stimulates caseinolytic activity | Candida albicans | |
SDS | stimulates chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide and peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-IE-(Ot-Bu)-AL aldehyde | 0.002 mg/ml, 75% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide. 0.002 mM, 8% inhibition of peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide | Candida albicans | |
benzyloxycarbonyl-LLL aldehyde | 0.001 mg/ml, 69% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide | Candida albicans | |
chymostatin | 0.05 mg/ml, 98% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide. 0.05 mg/ml, 15% inhibition of trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide | Candida albicans | |
leupeptin | 0.05 mg/ml, 25% inhibition of chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide. 0.05 mg/ml, 88% inhibition of trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide | Candida albicans | |
SDS | trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
gel filtration | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.228 | - |
- |
Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-GGL-4-nitroanilide + H2O | chymotrypsin-like activity | Candida albicans | ? | - |
? | |
benzyloxycarbonyl-GGR-2-naphthylamide + H2O | trypsin-like activity | Candida albicans | ? | - |
? | |
benzyloxycarbonyl-LLE-2-naphthylamide + H2O | peptidyl-glutamyl-peptide-hydrolyzing activity | Candida albicans | ? | - |
? | |
casein + H2O | - |
Candida albicans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | thre proteasomal subunits are identified as alpha6/Pre5, alpha3/Y13 and alpha5/Pup2 by internal sequencing of tryptic fragments. A fourth subunit is identified as alpha7/Prs1 by immunorecognition with a monoclonal antibody specific for C8 | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
20S proteasome | - |
Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | chymotrypsin-like activity, hydrolysis of benzyloxycarbonyl-GGL-4-nitroanilide and peptidyl-glutamyl-peptide-hydrolyzing activity, hydrolysis of benzyloxycarbonyl-LLE-2-naphthylamide | Candida albicans |
9 | - |
trypsin-like activity, hydrolysis of benzyloxycarbonyl-GGR-2-naphthylamide | Candida albicans |