BRENDA - Enzyme Database
show all sequences of 3.4.24.84

Prelamin A endoproteolytic processing in vitro by recombinant Zmpste24

Corrigan, D.P.; Kuszczak, D.; Rusinol, A.E.; Thewke, D.P.; Hrycyna, C.A.; Michaelis, S.; Sinensky, M.S.; Biochem. J. 387, 129-138 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Sf-21 insect cells; expression in CHO-K1 cells; Zmpste24, fused N-terminally to a His6 tag, cloned into the (5')-NheI-(3')-BamHI site of the pcDNA3.1 construct using reverse transcriptase-PCR on HeLa RNA, when cloned into pBacpak8
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
L647R
prelaminaAct mutant, cannot be cleaved by Zmpste24
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
Zn2+ chelator
Homo sapiens
lovastatin
abolishes the conversion of prelamin A into lamin A
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
membrane
-
Homo sapiens
16020
-
nuclear envelope
-
Homo sapiens
5635
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
Zn2+-dependent active site
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
SDS-PAGE or immunoblot analyis
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-bead-purified
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a-factor + H2O
the enzyme has CAAX endopeptidase activity towards a-factor substrate
667497
Homo sapiens
?
-
-
-
?
N-(Ac)-Cys-(farnesyl)-Ser-Ile-Met + H2O
the enzyme can process the prelaminA-specific CAAX sequence
667497
Homo sapiens
?
-
-
-
?
prelamin A + H2O
cleavage is dependent on processing at the CAAX-box
667497
Homo sapiens
lamin A
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Sf-21 insect cells; expression in CHO-K1 cells; Zmpste24, fused N-terminally to a His6 tag, cloned into the (5')-NheI-(3')-BamHI site of the pcDNA3.1 construct using reverse transcriptase-PCR on HeLa RNA, when cloned into pBacpak8
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L647R
prelaminaAct mutant, cannot be cleaved by Zmpste24
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
Zn2+ chelator
Homo sapiens
lovastatin
abolishes the conversion of prelamin A into lamin A
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
membrane
-
Homo sapiens
16020
-
nuclear envelope
-
Homo sapiens
5635
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
Zn2+-dependent active site
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
SDS-PAGE or immunoblot analyis
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-bead-purified
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a-factor + H2O
the enzyme has CAAX endopeptidase activity towards a-factor substrate
667497
Homo sapiens
?
-
-
-
?
N-(Ac)-Cys-(farnesyl)-Ser-Ile-Met + H2O
the enzyme can process the prelaminA-specific CAAX sequence
667497
Homo sapiens
?
-
-
-
?
prelamin A + H2O
cleavage is dependent on processing at the CAAX-box
667497
Homo sapiens
lamin A
-
-
-
?
Other publictions for EC 3.4.24.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719313
Ahmad
Homozygous null mutations in Z ...
Homo sapiens
Clin. Genet.
81
158-164
2012
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735044
Barrowman
Requirements for efficient pro ...
Homo sapiens
PLoS ONE
7
e32120
2012
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1
1
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9
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710803
Smigiel
Novel frameshifting mutations ...
Homo sapiens
Am. J. Med. Genet. A
152A
447-452
2010
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1
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1
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718809
Goulbourne
HIV protease inhibitors inhibi ...
Homo sapiens
Biochem. Soc. Trans.
38
292-296
2010
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1
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5
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1
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1
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712629
Rivas
Accelerated features of age-re ...
Mus musculus
J. Gerontol. A Biol. Sci. Med. Sci.
64
1015-1024
2009
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2
2
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678447
Porter
Inhibition of the CaaX proteas ...
Arabidopsis thaliana, Homo sapiens, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1773
853-862
2007
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6
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3
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682549
Coffinier
HIV protease inhibitors block ...
Mus musculus
Proc. Natl. Acad. Sci. USA
104
13432-13437
2007
-
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2
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4
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667497
Corrigan
Prelamin A endoproteolytic pro ...
Homo sapiens
Biochem. J.
387
129-138
2005
-
-
1
-
1
-
2
-
3
1
1
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2
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1
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669751
Moulson
Homozygous and compound hetero ...
Homo sapiens
J. Invest. Dermatol.
125
913-919
2005
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1
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670378
Varela
Accelerated ageing in mice def ...
Mus musculus
Nature
437
564-568
2005
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1
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1
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4
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638978
Bracha
The Arabidopsis AtSTE24 is a C ...
Arabidopsis thaliana, Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
277
29856-29864
2002
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1
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2
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1
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9
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3
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1
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1
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3
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3
1
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638977
Tam
The multispanning membrane pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
46798-46806
2001
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1
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2
2
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2
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1
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652151
Leung
Biochemical studies of Zmpste2 ...
Mus musculus
J. Biol. Chem.
276
29051-29058
2001
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1
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1
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4
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654005
Pei
Type II CAAX prenyl endopeptid ...
Saccharomyces cerevisiae
Trends Biochem. Sci.
26
275-277
2001
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1
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1
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638976
Schmidt
Reconstitution of the Ste24p-d ...
Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
275
6227-6233
2000
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653214
Trueblood
The CaaX proteases, Afc1p and ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
20
4381-4392
2000
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638974
Tam
Dual roles for Ste24p in yeast ...
Homo sapiens, Saccharomyces cerevisiae
J. Cell Biol.
142
635-649
1998
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638975
Schmidt
Endoplasmic reticulum membrane ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
95
11175-11180
1998
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651423
Boyartchuk
Roles pf prenyl protein protea ...
Saccharomyces cerevisiae
Genetics
150
95-1001
1998
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2
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638973
Fujimura-Kamada
A novel membrane-associated me ...
Saccharomyces cerevisiae
J. Cell Biol.
136
271-285
1997
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1
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3
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1
1
1
1
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6
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2
1
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653884
Boyartchuk.V.L.; Ashby
Modulation of Ras and a-factor ...
Saccharomyces cerevisiae
Science
275
1796-1800
1997
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1
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