Literature summary for 3.4.24.40 extracted from

Chessa, J.P.; Petrescu, I.; Bentahir, M.; Van Beeumen, J.; Gerday, C.
Purification, physico-chemical characterization and sequence of a heat labile alkaline metalloprotease isolated from a psychrophilic Pseudomonas species (2000), Biochim. Biophys. Acta, 1479, 265-274.

Search for more literature for 3.4.24.40

Cloned(Commentary)

Cloned (Comment)	Organism
-	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Pseudomonas sp.
EDTA	10 mM, pH 8, strong inhibition	Pseudomonas sp.
EGTA	10 mM, pH 8, strong inhibition	Pseudomonas sp.
HgCl2	complete inhibition	Pseudomonas sp.
additional information	not inhibited by dithiothreitol, PMSF and DFP	Pseudomonas sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Pseudomonas sp.	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stabilizing	Pseudomonas sp.
Zn2+	-	Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48690	-	electrospray mass spectrometry	Pseudomonas sp.
48690	-	calculated	Pseudomonas sp.
48730	-	electrospray mass spectrometry	Pseudomonas sp.
48770	-	electrospray mass spectrometry	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-
Pseudomonas sp. TAC-II-18	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	113 U/mg, one unit gives an increase of 0.01 absorbance at 366 nm, excess of azocasein in 20 mM Tris, 1 mM CaCl2, pH 8	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
azocasein + H2O	-	Pseudomonas sp.	?	-	?
azocasein + H2O	-	Pseudomonas sp. TAC-II-18	?	-	?

Synonyms

Synonyms	Comment	Organism
psychrophilic alkaline metalloprotease	PAP	Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4	-	highest acitivity after 4 days of culture	Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	50% activity after 20 min	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	10	-	Pseudomonas sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)