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Literature summary for 3.4.24.26 extracted from
- Pseudomonas protease. Purification, partial characterization, and its effect on collagen, proteoglycan, and rabbit corneas (1977), Invest. Ophthalmol. Vis. Sci., 16, 488-497.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | complete inhibition at 1 mM | Pseudomonas aeruginosa |  |
| Co2+ | 50% inhibition at 0.625 mM | Pseudomonas aeruginosa | |
| Cu2+ | complete inhibition | Pseudomonas aeruginosa | |
| Fe3+ | 75% inhibition at 0.625 mM | Pseudomonas aeruginosa | |
| L-cysteine | complete inhibition at 1.25 mM | Pseudomonas aeruginosa | |
| Mn2+ | 30% inhibition at 0.625 mM | Pseudomonas aeruginosa | |
| additional information | no inhibition by diisopropylphosphofluoridate at 20 mM | Pseudomonas aeruginosa | |
| Na2 EDTA | complete inhibition at 10 mM | Pseudomonas aeruginosa | |
| Zn2+ | complete inhibition | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+ and Ca2+ at 0.625 mM have no effect on the activity, Mn2+, Co2+, and Fe3+ at 0.625 mM partly inhibit the enzyme, Zn2+ and Cu2+ inhibit the enzyme completely | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-L-alanyl-L-alanyl-L-alanine-methyl ester + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| azocasein + H2O | pH 7.5, 37°C | Pseudomonas aeruginosa | ? | - |
? | |
| orcein-elastin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pseudomonas protease | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas aeruginosa |
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