Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.13 extracted from

  • Vitovski, S.; Sayers, J.R.
    Relaxed cleavage specificity of an immunoglobulin A1 protease from Neisseria meningitidis (2007), Infect. Immun., 75, 2875-2885.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Neisseria meningitidis

Protein Variants

Protein Variants Comment Organism
DELTA1003-1006 mutant lacking the confirmed self-cleavage recognition site shows only little reduction in secreted protease Neisseria meningitidis
DELTA1005-1006 mutant containing modified consensus recognition sites shows only slight reduction in protease secretion Neisseria meningitidis
DELTA974-1002 mutant lacking the putative self-cleavage recognition site and the intervening residues shows only little reduction in secreted protease Neisseria meningitidis
DELTA974-978 mutant lacking the putative self-cleavage recognition site shows only little reduction in secreted protease Neisseria meningitidis
DELTA974-978/DELTA1003-1007 double deletion mutant lacking both self-cleavage recognition sites shows only a 40% reduced secretion Neisseria meningitidis
additional information a mutant in which the entire region spanning the putative (residues 974-978) and the confirmed self-cleavage site (residues 1003-1006) is replaced by a hexahistidine linker fails to produce any detectable secreted protease Neisseria meningitidis
P1004T/S1005E/S1006A mutant containing modified consensus recognition sites shows only slight reduction in protease secretion Neisseria meningitidis
P975E/P1004E mutant containing modified consensus recognition sites shows 30% lower secreted protease levels than wild-type Neisseria meningitidis
S1005E mutant containing modified consensus recognition sites shows only slight reduction in protease secretion Neisseria meningitidis
S267V this mutant fails to undergo self-cleavage and shows no enzymatic activity Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-
Neisseria meningitidis NMB
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the unexpected self-cleavage and subsequent extracellular release of mature IgA1 protease from mutants lacking the previously defined consensus cleavage site (P-P-S-P, residues 1003-1006) and/or the putative (P-V-P-S-P, residues 1003-1006) self-cleavage site is shown Neisseria meningitidis

Synonyms

Synonyms Comment Organism
IgA1 protease
-
Neisseria meningitidis