Literature summary for 3.4.23.48 extracted from

Agarkov, A.; Chauhan, S.; Lory, P.J.; Gilbertson, S.R.; Motin, V.L.
Substrate specificity and screening of the integral membrane protease Pla (2008), Bioorg. Med. Chem. Lett., 18, 427-431.

Search for more literature for 3.4.23.48

Cloned(Commentary)

Cloned (Comment)	Organism
BL21-DE3 used as a host to express recombinant protein, cloning into pBluescript SK+ to produce plasmid pBLA4L	Yersinia pestis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	potential small-peptide inhibitors of plasminogen activator Pla screened by parallel synthesis and positional scanning, selected small-peptide substrates analyzed by examining ability to inhibit the conversion of plasminogen to plasmin in a fibrin plate assay and by application of a fluorimetric assay	Yersinia pestis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	outer membrane	Yersinia pestis	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
plasminogen + H2O	Yersinia pestis	-	plasmin + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Yersinia pestis	P17811	non-pigmented avirulent strain of Yersinia pestis KIM, substrain D28, expressing plasminogen activator Pla and its pPCP-cured version, substrain D47	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant plasminogen activator Pla, gel filtration	Yersinia pestis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	peptide synthesis described, DABCYL/EDANS quencher/fluorophor system used, minimal substrate length determined, positional scan approach applied, peptide sequences tested as substrates and functional inhibitors listed, kinetics of cleavage of identified fluorescent substrates shown, fluorimetric assay and fibrinolytic assay applied, analysis of six-residue peptides and optimization studies of potential tripeptide substrates performed, inhibitory activity od selectetes small-peptides analyzed	Yersinia pestis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
plasminogen + H2O	-	Yersinia pestis	plasmin + ?	-	?
plasminogen + H2O	identification and analysis of small-peptide substrates and inhibitors of plasminogen activator Pla by use of parallel synthesis and positional scanning	Yersinia pestis	plasmin + ?	-	?

Synonyms

Synonyms	Comment	Organism
plasminogen activator Pla	-	Yersinia pestis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	fibrinolysin assay	Yersinia pestis

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Release 2023.1 (January 2023)