BRENDA - Enzyme Database show
show all sequences of 3.4.23.36

Signal peptidase II

Sankaran, K.; Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) 1, 201-204 (2004)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
detergent
required for activity, e.g. Triton X-100 or Nikkol, but not lauryl sarcoside or octylglucoside
Escherichia coli
Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Klebsiella aerogenes
DNA and amino acid sequence determination and analysis
Pseudomonas fluorescens
DNA and amino acid sequence determination and analysis
Staphylococcus aureus
enzyme overexpression in Escherichia coli confers high resistance to globomycin
Myxococcus xanthus
gene lsp, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli under control of the trp promoter
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
Globomycin
-
Bacillus subtilis
Globomycin
uncompetitive
Escherichia coli
Globomycin
-
Klebsiella aerogenes
Globomycin
-
Myxococcus xanthus
Globomycin
-
Pseudomonas fluorescens
Globomycin
-
Staphylococcus aureus
HgCl2
-
Escherichia coli
lauroyl sarcoside
-
Escherichia coli
octylglucoside
-
Escherichia coli
tosyl arginyl methyl ester
-
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
inner membrane
-
Bacillus subtilis
-
-
inner membrane
transmembrane protein with a large and a small loop at the periplasmic side
Escherichia coli
-
-
inner membrane
-
Klebsiella aerogenes
-
-
inner membrane
-
Myxococcus xanthus
-
-
inner membrane
-
Pseudomonas fluorescens
-
-
inner membrane
-
Staphylococcus aureus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Bacillus subtilis
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Escherichia coli
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Klebsiella aerogenes
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Myxococcus xanthus
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Pseudomonas fluorescens
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Staphylococcus aureus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE
Escherichia coli
18144
-
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bacterial lipoprotein + H2O
Staphylococcus aureus
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Bacillus subtilis
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Escherichia coli
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Pseudomonas fluorescens
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Klebsiella aerogenes
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Myxococcus xanthus
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
gene lsp
-
Klebsiella aerogenes
-
-
-
Myxococcus xanthus
-
-
-
Pseudomonas fluorescens
-
-
-
Staphylococcus aureus
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme
Escherichia coli
proteolytic modification
N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme
Escherichia coli
Purification (Commentary)
Commentary
Organism
native enzyme from inner membrane to homogeneity by solubilization from membrane with Triton X-100, heat treatmemt at 65C and pH 4.0, anion exchange chromatography, and chromatofocusing, 35000fold, recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography in presence of EDTA, and gel filtration to homogeneity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Staphylococcus aureus
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Bacillus subtilis
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Escherichia coli
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Pseudomonas fluorescens
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Klebsiella aerogenes
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Myxococcus xanthus
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Staphylococcus aureus
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Bacillus subtilis
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Escherichia coli
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Pseudomonas fluorescens
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Klebsiella aerogenes
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Myxococcus xanthus
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Staphylococcus aureus
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Bacillus subtilis
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Escherichia coli
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Pseudomonas fluorescens
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Klebsiella aerogenes
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Myxococcus xanthus
?
-
-
-
?
additional information
active site catalytic residues are Asp102 and Asp129
668802
Bacillus subtilis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE
Escherichia coli
More
primary structure
Escherichia coli
More
primary structure
Klebsiella aerogenes
More
primary structure
Pseudomonas fluorescens
More
primary structure
Staphylococcus aureus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
80
-
purified enzyme, stable for a short time
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
neutral pH optimum
Bacillus subtilis
additional information
-
neutral pH optimum
Klebsiella aerogenes
additional information
-
neutral pH optimum
Myxococcus xanthus
additional information
-
neutral pH optimum
Pseudomonas fluorescens
additional information
-
neutral pH optimum
Staphylococcus aureus
6
-
purified enzyme
Escherichia coli
7.9
-
crude enzyme
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no requirement for cofactors
Bacillus subtilis
additional information
no requirement for cofactors
Escherichia coli
additional information
no requirement for cofactors
Klebsiella aerogenes
additional information
no requirement for cofactors
Myxococcus xanthus
additional information
no requirement for cofactors
Pseudomonas fluorescens
additional information
no requirement for cofactors
Staphylococcus aureus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000036
-
Globomycin
-
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
detergent
required for activity, e.g. Triton X-100 or Nikkol, but not lauryl sarcoside or octylglucoside
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Klebsiella aerogenes
DNA and amino acid sequence determination and analysis
Pseudomonas fluorescens
DNA and amino acid sequence determination and analysis
Staphylococcus aureus
enzyme overexpression in Escherichia coli confers high resistance to globomycin
Myxococcus xanthus
gene lsp, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli under control of the trp promoter
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no requirement for cofactors
Bacillus subtilis
additional information
no requirement for cofactors
Escherichia coli
additional information
no requirement for cofactors
Klebsiella aerogenes
additional information
no requirement for cofactors
Myxococcus xanthus
additional information
no requirement for cofactors
Pseudomonas fluorescens
additional information
no requirement for cofactors
Staphylococcus aureus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Globomycin
-
Bacillus subtilis
Globomycin
uncompetitive
Escherichia coli
Globomycin
-
Klebsiella aerogenes
Globomycin
-
Myxococcus xanthus
Globomycin
-
Pseudomonas fluorescens
Globomycin
-
Staphylococcus aureus
HgCl2
-
Escherichia coli
lauroyl sarcoside
-
Escherichia coli
octylglucoside
-
Escherichia coli
tosyl arginyl methyl ester
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000036
-
Globomycin
-
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
inner membrane
-
Bacillus subtilis
-
-
inner membrane
transmembrane protein with a large and a small loop at the periplasmic side
Escherichia coli
-
-
inner membrane
-
Klebsiella aerogenes
-
-
inner membrane
-
Myxococcus xanthus
-
-
inner membrane
-
Pseudomonas fluorescens
-
-
inner membrane
-
Staphylococcus aureus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Bacillus subtilis
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Escherichia coli
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Klebsiella aerogenes
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Myxococcus xanthus
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Pseudomonas fluorescens
additional information
no requirement for metal ions, thiol reagents, and phospholipids
Staphylococcus aureus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18000
-
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE
Escherichia coli
18144
-
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bacterial lipoprotein + H2O
Staphylococcus aureus
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Bacillus subtilis
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Escherichia coli
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Pseudomonas fluorescens
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Klebsiella aerogenes
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
bacterial lipoprotein + H2O
Myxococcus xanthus
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
?
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme
Escherichia coli
proteolytic modification
N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from inner membrane to homogeneity by solubilization from membrane with Triton X-100, heat treatmemt at 65C and pH 4.0, anion exchange chromatography, and chromatofocusing, 35000fold, recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography in presence of EDTA, and gel filtration to homogeneity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Staphylococcus aureus
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Bacillus subtilis
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Escherichia coli
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Pseudomonas fluorescens
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Klebsiella aerogenes
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis
668802
Myxococcus xanthus
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Staphylococcus aureus
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Bacillus subtilis
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Escherichia coli
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Pseudomonas fluorescens
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Klebsiella aerogenes
?
-
-
-
?
bacterial lipoprotein + H2O
cleavage of the signal peptide
668802
Myxococcus xanthus
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Staphylococcus aureus
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Bacillus subtilis
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Escherichia coli
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Pseudomonas fluorescens
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Klebsiella aerogenes
?
-
-
-
?
Braun's polylipoprotein + H2O
cleavage of the signal peptide, cleavage site specificity, overview
668802
Myxococcus xanthus
?
-
-
-
?
additional information
active site catalytic residues are Asp102 and Asp129
668802
Bacillus subtilis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE
Escherichia coli
More
primary structure
Escherichia coli
More
primary structure
Klebsiella aerogenes
More
primary structure
Pseudomonas fluorescens
More
primary structure
Staphylococcus aureus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
80
-
purified enzyme, stable for a short time
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
neutral pH optimum
Bacillus subtilis
additional information
-
neutral pH optimum
Klebsiella aerogenes
additional information
-
neutral pH optimum
Myxococcus xanthus
additional information
-
neutral pH optimum
Pseudomonas fluorescens
additional information
-
neutral pH optimum
Staphylococcus aureus
6
-
purified enzyme
Escherichia coli
7.9
-
crude enzyme
Escherichia coli
Other publictions for EC 3.4.23.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717100
Xiao
Myxobacteria antibiotic TA (my ...
Escherichia coli
Antimicrob. Agents Chemother.
56
2014-2021
2012
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
708786
Schmaler
Staphylococcal lipoproteins an ...
Staphylococcus aureus
Int. J. Med. Microbiol.
300
155-160
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708937
Vickerman
A genetic determinant in Strep ...
Streptococcus gordonii
J. Bacteriol.
192
2535-2545
2010
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
707166
Banaei
Lipoprotein processing is esse ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Antimicrob. Agents Chemother.
53
3799-3802
2009
-
-
-
-
-
-
-
-
-
-
-
2
-
163
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
708925
Das
Contribution of lipoproteins a ...
Streptococcus sanguinis, Streptococcus sanguinis SK36
J. Bacteriol.
191
4166-4179
2009
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
682007
Khandavilli
Maturation of Streptococcus pn ...
Streptococcus pneumoniae
Mol. Microbiol.
67
541-557
2008
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700533
Arimoto
Role of prolipoprotein diacylg ...
Streptococcus mutans
Oral Microbiol. Immunol.
23
515-519
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680486
Rahman
The lspA gene, encoding the ty ...
Rickettsia typhi
J. Bacteriol.
189
336-341
2007
-
-
1
-
-
-
-
-
1
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682822
Geukens
The type II signal peptidase o ...
Legionella pneumophila
Res. Microbiol.
157
836-841
2006
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668802
Sankaran
-
Signal peptidase II ...
Bacillus subtilis, Escherichia coli, Klebsiella aerogenes, Myxococcus xanthus, Pseudomonas fluorescens, Staphylococcus aureus
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
201-204
2004
1
-
5
-
-
-
10
-
6
6
2
6
-
6
-
2
1
-
-
-
-
-
19
5
-
-
1
-
7
-
-
6
1
-
-
1
-
5
6
-
-
-
-
10
1
-
6
6
2
6
-
-
2
1
-
-
-
-
19
5
-
-
1
-
7
-
-
-
-
-
-
-
-
-
670307
Sander
Lipoprotein processing is requ ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
52
1543-1552
2004
-
-
1
-
1
-
-
-
-
-
-
2
-
161
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670851
Gonnet
Fine-tuning the prediction of ...
Escherichia coli K-12
Proteomics
4
1597-1613
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651951
Tjalsma
The potential active site of t ...
Bacillus subtilis
J. Biol. Chem.
274
28191-28197
1999
-
-
1
-
16
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30813
Sankaran
Bacterial prolipoprotein signa ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
248
169-180
1995
2
-
1
-
-
1
9
1
1
1
1
2
-
177
-
-
1
-
-
-
-
1
6
-
1
1
1
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
9
-
1
1
1
1
2
-
-
-
1
-
-
-
1
6
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
30814
Zhao
Nucleotide sequence of the Sta ...
Staphylococcus aureus
FEBS Lett.
299
80-84
1992
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30809
Isaki
Cloning and nucleotide sequenc ...
Klebsiella aerogenes
J. Bacteriol.
172
469-472
1990
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30812
Dev
Signal peptidases and signal p ...
Escherichia coli
J. Bioenerg. Biomembr.
22
271-290
1990
2
-
-
-
-
-
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30808
Innis
Nucleotide sequence of the Esc ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
81
3708-3712
1984
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30810
Yu
Nucleotide sequence of the lsp ...
Escherichia coli
FEBS Lett.
173
264-268
1984
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30811
Yamada
The major outer membrane lipop ...
Escherichia coli
FEBS Lett.
166
179-182
1984
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-