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Literature summary for 3.4.22.51 extracted from

  • dos Reis, F.C.; Judice, W.A.; Juliano, M.A.; Juliano, L.; Scharfstein, J.; Lima, A.P.
    The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi (2006), FEMS Microbiol. Lett., 259, 215-220.
    View publication on PubMed
Search for more literature for 3.4.22.51

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 o-aminobenzoyl-HLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0022
-
 o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0028
-
 o-aminobenzoyl-KLFFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0032
-
 o-aminobenzoyl-KRRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0036
-
 o-aminobenzoyl-LLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0038
-
 o-aminobenzoyl-KLRLSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.004
-
 o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0044
-
 o-aminobenzoyl-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0046
-
 o-aminobenzoyl-KLRFFKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0046
-
 o-aminobenzoyl-KLRFSRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0051
-
 o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0051
-
 o-aminobenzoyl-KFRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0058
-
 epsilon-NH2(capryl)-L-(SBzl)C-7-amido-4-methylcoumarin
-
 Trypanosoma cruzi
0.006
-
 o-aminobenzoyl-KARFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0073
-
 o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0078
-
 o-aminobenzoyl-KLRFSPQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0079
-
 o-aminobenzoyl-KLKFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0079
-
 o-aminobenzoyl-KLRFPKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0114
-
 o-aminobenzoyl-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0117
-
 o-aminobenzoyl-KPRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
0.0132
-
 o-aminobenzoyl-KLRRSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epsilon-NH2(capryl)-L-(SBzl)C-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi ?
-
 ?
additional information substrate specificity of cruzipain 2 isoform is analysed using internally quenched flurogenic substrates. It is found that cruzipain 2 compared to cruzipain differs substantially regarding the specificity in the S2, S1' and S2' pockets, suggesting that cruzipain 2 acts on distinct natural substrates Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-HLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-KARFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-KFRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-KLFFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLF + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLG + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLKFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLK + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + FAKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFFKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + FFKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFPKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + FPKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFSFQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O no hydrolysis is detected Trypanosoma cruzi o-aminobenzoyl-KLR + FSFQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + FSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFSPQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + FSPQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRFSRQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + FSRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRLSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + LSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KLRRSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi o-aminobenzoyl-KLR + RSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KPRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-KRRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?
o-aminobenzoyl-LLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Trypanosoma cruzi ?
-
 ?

Synonyms

Synonyms Comment Organism
cruzipain 2
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 o-aminobenzoyl-KPRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
1.3
-
 o-aminobenzoyl-KARFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
1.9
-
 o-aminobenzoyl-KFRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
1.9
-
 o-aminobenzoyl-KLRRSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
2.5
-
 o-aminobenzoyl-KLFFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
2.8
-
 o-aminobenzoyl-KLRFPKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
4.3
-
 o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
4.5
-
 o-aminobenzoyl-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
5.6
-
 o-aminobenzoyl-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
5.6
-
 o-aminobenzoyl-HLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
6.7
-
 o-aminobenzoyl-LLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
6.9
-
 o-aminobenzoyl-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
7.7
-
 o-aminobenzoyl-KRRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
8.2
-
 o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
9.1
-
 o-aminobenzoyl-KLRLSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
9.7
-
 o-aminobenzoyl-KLRFFKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
10.8
-
 epsilon-NH2(capryl)-L-(SBzl)C-7-amido-4-methylcoumarin
-
 Trypanosoma cruzi
12.5
-
 o-aminobenzoyl-KLRFSRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
13.9
-
 o-aminobenzoyl-KLRFSPQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
14.2
-
 o-aminobenzoyl-KLKFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi
15.2
-
 o-aminobenzoyl-KLRFAKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Trypanosoma cruzi