Cloned (Comment) | Organism |
---|---|
inserted in pET20b(+), transformed into the Escherichia coli BL21(DE3)pLysS strain | Fasciola gigantica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | concentration of 1 mM, 10 min before addition of substrate, 40% inhibition; concentration of 1mM, 10 min before addition of substrate, 40% inhibition observed | Fasciola gigantica | |
benzyloxycarbonyl-L-Ala-L-Ala-azaAsn-chloromethylketone | Z-Ala-Ala-AzaAsn-CMK, 1 microM, 10 min before addition of substrate, 100% inhibition; Z-Ala-Ala-AzaAsn-CMK, 1 microM, 10 min before addition of substrate, 100% inhibition observed | Fasciola gigantica | |
iodoacetamide | concentration of 5 mM,10 min before addition of substrate, 100% inhibition; concentration of 5 mM, 10 min before addition of substrate, 100% inhibition determined | Fasciola gigantica | |
additional information | no significant inhibition by trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane (E-64) in a concentration of 10 microM, leupeptin in a concentration of 10 microM or pepstatin in a concentration of 5 microM; no significant inhibition observed by using trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane (E-64) in a concentration of 10 microM, leupeptin in a concentration of 10 microM or pepstatin in a concentration of 5 microM | Fasciola gigantica | |
phenylmethanesulfonyl fluoride | PMSF, concentration of 2 mM, 10 min before addition of substrate, 66% inhibition; PMSF, concentration of 2 mM, 10 min before addition of substrate, 66% inhibition shown | Fasciola gigantica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | crude worm protein extracts but not in excretion-secretion products of adult parasites, polyclonal antisera and activity assay | Fasciola gigantica | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola gigantica | A6Y9U8 | legumain-1, differentiated from legumain-2 by N-terminal sequence; collected from bile ducts and gall bladders of naturally infected cattle | - |
Fasciola gigantica | A6Y9U9 | legumain-2, differentiated from legumain-1 by N-terminal sequence; collected from bile ducts and gall bladders of naturally infected cattle | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | N-terminal and C-terminal cleavage sites predicted, N-terminal sequence | Fasciola gigantica |
proteolytic modification | N-terminal and C-terminal cleavage sites predicted, N-terminal sequence indicated | Fasciola gigantica |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, gel filtration and SDS-PAGE | Fasciola gigantica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestinal epithelium | caecal epithelium, 2-6 week old juveniles and adults, mRNA expression also in metacercariae | Fasciola gigantica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity profile shown in crude worm protein extracts but not in excretion-secretion products of adult parasites, inhibition assay described, exon boundaries, landmark residues/motifs, and target areas for N- and C-terminal processing indicated | Fasciola gigantica |
additional information | - |
activity profile shown in crude worm protein extracts but not in excretion-secretion products of adult parasites, inhibition assay, exon boundaries, landmark residues/motifs, and target areas for N- and C-terminal processing | Fasciola gigantica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Asn-4-nitroanilide + H2O | Bz-Asn-pNA, recombinant protein, at 37°C | Fasciola gigantica | benzoyl-Asn + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AEP | - |
Fasciola gigantica |
Asparaginyl endopeptidase | - |
Fasciola gigantica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay at | Fasciola gigantica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
activity assay at, maximum of activity observed at pH 5-6 | Fasciola gigantica |
5.4 | - |
activity assay at, maximum of activity observed at pH 56 | Fasciola gigantica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | assay under different pH conditions tested in 0.2 M sodium citrate/citric acid, 1 mM DTT at 37°C, no enzyme activity observed at pH 4 and below, activity dropped to 55% at pH 7 | Fasciola gigantica |
2 | 7 | assay under different pH conditions tested in 0.2 M sodium citrate/citric acid, 1 mM DTT at 37°C, no enzyme activity observed at pH 4 and below, activity drops to 55% at pH 7 | Fasciola gigantica |