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Information on EC 3.4.22.34 - Legumain
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3.4.22.34 LegumainSpecify your search results
Word Map
- 3.4.22.34
- cathepsins
- phaseolus
- vulgaris
- vacuole
- cystatins
- proteinases
- metastasis
- papain
- cotyledon
- plasmodium
- globulin
- falciparum
- vicilins
- schistosoma
-
papain-like
- tau
-
legume
- endopeptidases
-
seed-specific
-
mesoamerican
- phytohemagglutinin
- falcipain-2
-
clan
- mansoni
-
andean
-
leguminous
-
procathepsins
- lunatus
-
cyclotides
-
jack
-
caspase-1-like
- drug development
- biotechnology
- medicine
- diagnostics
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
legumain, phaseolin, asparaginyl endopeptidase, lysosomal cysteine proteinase, vacuolar processing enzyme, hemoglobinase, asparagine endopeptidase, vicilin peptidohydrolase, hllgm, hllgm2, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AEP
Antigen Sj32
Antigen SM32
Asn-specific vacuolar cysteine proteinase
asparagine endopeptidase
Asparaginyl endopeptidase
Bean endopeptidase
-
-
-
-
hemoglobinase
HlLgm2
legumain
LGMN
lysosomal cysteine proteinase
Phaseolin
-
-
-
-
Proteinase B
schistosome legumain
vacuolar processing enzyme
Vicilin peptidohydrolase
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-
-
-
VPE
additional information
Antigen Sj32
-
-
-
-
Antigen SM32
-
-
-
-
Asparaginyl endopeptidase
-
-
-
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Proteinase B
-
-
-
-
additional information
the enzyme belongs to the C13 peptidase family
additional information
the enzyme belongs to the C13 peptidase family
additional information
the enzyme belongs to the C13 peptidase family
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
-
-
-
-
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
best known from legume seeds, the trematode Schistosoma mansoni and mammalian lysosomes. Not inhibited by compound E-64. Type example of peptidase family C13
-
-
-
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
active site His151
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
active site His151 and Cys197
hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
the enzyme contains essential catalytic His and Cys residues
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hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
the enzyme contains essential catalytic His and Cys residues
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hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds
the enzyme contains essential catalytic His and Cys residues
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
149371-18-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Ac-Asp-Asn-Leu-Asp alpha-(4-methylcoumaryl-7-amide) + H2O
Ac-Asp-Asn-Leu-Asp + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-Pro-Thr-Asn-4-methylcoumarin-7-amide + H2O
acetyl-Pro-Thr-Asn + 7-amino-4-methylcoumarin
acetyl-Thr-Ala-Asn-4-methylcoumarin-7-amide + H2O
acetyl-Thr-Ala-Asn + 7-amino-4-methylcoumarin
acetyl-Tyr-Val-Ala-Asp-4-methylcoumarin-7-amide + H2O
acetyl-Tyr-Val-Ala-Asp + 7-amino-4-methylcoumarin
AGTHNGQIGA + H2O
AGTHN + GQIGA
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the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived, low activity
-
?
AHIDNEEDIA + H2O
AHIDN + EEDIA
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low activity, the peptide sequence is also cleaved in tetanus toxoid C fragment of which it is derived
-
?
ALKGNNLIWA + H2O
ALKGN + NLIWA
-
the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
-
?
annexin II + H2O
?
cleavage of the N-terminus, enzyme plays a role in inactivation and degradation of annexin II in endosomes and lysosomes
-
?
AQLKNITDYA + H2O
AQLKN + ITDYA
-
the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
-
?
AREDNNITLA + H2O
AREDN + NITLA
-
low activity, the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
-
?
ARLYNGLKFA + H2O
ARLYN + GLKFA
-
the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
-
?
ASGFNSSVIA + H2O
ASGFN + SSVIA
-
the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
-
?
ATITNDRLSA + H2O
ATITN + DLRSA
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the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
-
?
AYGTNEYSIA + H2O
AYGTN + EYSIA
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the peptide sequence is not cleaved in tetanus toxoid C fragment of which it is derived
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?
benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-(tert-butyl)Tyr-Ala-Asn + 7-amino-4-methylcoumarin
Benzyloxycarbonyl-Ala 4-nitrophenyl ester + H2O
Benzyloxycarbonyl-Ala + 4-nitrophenol
-
-
-
?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcouamrin-7-amide + H2O
benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
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-
-
?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Ala-Ala-Asp-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Ala-Ala-Asp + 7-amino-4-methylcoumarin
the canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp
-
-
?
benzyloxycarbonyl-Ala-Glu-Asn-Xaa-Ala-Glu-Lys-NH2 + H2O
benzyloxycarbonyl-Ala-Glu-Asn + Xaa-Ala-Glu-Lys-NH2
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substrate specificity at S1 position, overview
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?
Benzyloxycarbonyl-Ala-Pro-Tyr-Asn 4-methylcoumarin 7-amide + H2O
?
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-
-
-
?
Benzyloxycarbonyl-Asn nitrophenyl ester + H2O
Benzyloxycarbonyl-Asn + 4-nitrophenol
-
-
-
?
Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O
Benzyloxycarbonyl-Gly + 4-nitrophenol
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-
-
?
benzyloxycarbonyl-Gly-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Gly-Ala-Asn + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Ile-Ser-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Ile-Ser-Asn + 7-amino-4-methylcoumarin
low activity with
-
?
benzyloxycarbonyl-Leu-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Leu-Ala-Asn + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Phe 4-nitrophenyl ester + H2O
benzyloxycarbonyl-Phe + 4-nitrophenol
-
-
-
?
benzyloxycarbonyl-Phe-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Phe-Ala-Asn + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Pro-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Pro-Ala-Asn + 7-amino-4-methylcoumarin
Benzyloxycarbonyl-Tyr 4-nitrophenyl ester + H2O
Benzyloxycarbonyl-Tyr + 4-nitrophenol
-
-
-
?
benzyloxycarbonyl-Tyr-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Tyr-Ala-Asn + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Val-Ala-Asn + 7-amino-4-methylcoumarin
bovine albumin + H2O
peptides containing C-terminal Asn
-
-
-
?
bovine haemoglobin + H2O
peptides containing C-terminal Asn
exhibited strict specificity for the asparaginyl bonds on the carboxy-terminal side of a peptide
-
-
?
bovine serum albumin + H2O
3 fragments
-
SDS-denatured substrate, cleavage site are at positions 324 and 404
-
?
carbobenzyloxy-Ala-Ala-Asn-ethylenediamine-etoposide + H2O
etoposide-ethylenediamine + carbobenzyloxy-Ala-Ala-Asn
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legumain releases the chemotherapeutic agent etoposide, as the active drug
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-
?
CBZ-Ala-Ala-Asn-amido-4-methylcoumarin + H2O
CBZ-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
-
-
-
?
concanavalin A A-chain + H2O
3 fragments
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cleavage site are at positions 159 and 163
-
?
concanavalin A precursor + H2O
activated concanavalin A
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internal excision of the propeptide and transpeptidation that leads to the inverted fusion of the 2 fragments of the precursor
-
?
GSVKAYTNFDAERD + H2O
GSVKAYTN + FDAERD
synthetic peptide, residues24-37, derived from the N-terminal sequence of bovine annexin II, cleavage at position N31-F32
-
?
legumain + H2O
processed legumain + N-terminal and C-terminal prodomains
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autocatalysis
-
?
lysozyme C + H2O
3 fragments
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cleavage site are at positions 62 and 64
-
?
myelin basic protein MBP + H2O
5 fragments
-
cleavage sites at positions 84-85 and 92-93, multiple-sclerosis-associated autoantigen, digestion, destruction of the immunodominant epitope 83-99
product determination
?
octapeptides basing on the processing sites of 11S proglobulin + H2O
?
-
-
-
?
papain-like cysteine proteinase precursors + H2O
activated papin-like cysteine proteinases
progelatinase A + H2O
gelatinase A + ?
-
in cell culture of human fibrosarcoma HT1080 cells, 72 kDa proform, activation by cleavage of an asparaginyl peptide bond at Asn109-Tyr110 or Asn111-Phe112 to form the N-terminus of the mature active gelatinase
62 kDa mature form
?
Proglycinin + H2O
?
-
cleavage takes place between the conserved Asn and Gly residues
-
-
?
Prolegumin + H2O
?
-
cleavage takes place between the conserved Asn and Gly residues
-
-
?
rat alpha1-macroglobulin + H2O
3 fragments
-
cleavage site are at positions 721 and 899
-
?
recombinant C-fragment of tetanus toxoid + H2O
?
-
cleavage of 3 peptide bonds
-
?
Ser-Glu-Ser-Glu-Asn-Gly-Leu-Glu-Glu-Thr + H2O
Ser-Glu-Ser-Glu-Asn + Gly-Leu-Glu-Glu-Thr
-
-
-
?