Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant CatB3 shows enzymatic activity when autoprocessed at pH 4.5 in the presence of dithiothreitol and dextran sulfate | Fasciola gigantica |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Fasciola gigantica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27900 | - |
mature enzyme, calculated from amino acid sequence | Fasciola gigantica |
34000 | - |
native cathepsin B3, SDS-PAGE | Fasciola gigantica |
37000 | - |
pro-CatB3, non-denaturing SDS-PAGE | Fasciola gigantica |
38000 | 39000 | deglycosylation of recombinant CatB3 with endoglycosidase H under denaturing and native conditions produces protein migrating at 38000-39000 Da | Fasciola gigantica |
39200 | - |
proenzyme, calculated from amino acid sequence | Fasciola gigantica |
55000 | 75000 | purified recombinant glycosylated proCatB3 forms migrate at approximately 55000-75000Da | Fasciola gigantica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibronectin + H2O | Fasciola gigantica | - |
? | - |
? | |
Gelatin + H2O | Fasciola gigantica | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola gigantica | Q86MW6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Fasciola gigantica |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Fasciola gigantica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
caecal epithelium | - |
Fasciola gigantica | - |
larva | cathepsin B3 is detected in the whole body extract of metacercariae and newly excysted juveniles but not in 4-week-old juveniles or adult parasites | Fasciola gigantica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Fibronectin + H2O | - |
Fasciola gigantica | ? | - |
? | |
Gelatin + H2O | - |
Fasciola gigantica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CatB3 | - |
Fasciola gigantica |
cathepsin B3 | - |
Fasciola gigantica |
General Information | Comment | Organism |
---|---|---|
physiological function | CatB3 is a major protease antigen in Fasciola gigantica metacercariae and newly excysted juveniles | Fasciola gigantica |