Information on EC 3.4.22.16 - cathepsin H

succinyl-Ala-Ala-Ala-4-nitroanilide + H2O

succinyl-Ala-Ala-Ala + 4-nitroaniline

succinyl-Ala-Ala-Ala-Ala-4-nitroanilide + H2O

succinyl-Ala-Ala-Ala-Ala + 4-nitroaniline

succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide + H2O

succinyl-Ala-Ala-Ala-Ala-Ala + 4-nitroaniline

succinyl-Ala-Ala-Pro-4-nitroanilide + H2O

succinyl-Ala-Ala-Pro + 4-nitroaniline

succinyl-Ala-Pro-Ala-4-nitroanilide + H2O

succinyl-Ala-Pro-Ala + 4-nitroaniline

succinyl-Pro-Ala-Ala-4-nitroanilide + H2O

succinyl-Pro-Ala-Ala + 4-nitroaniline

surfactant protein B + H2O

Mus musculus

718296

talin + H2O

cathepsin H cleaves the N-terminus of the talin F0 head domain

732111

tropomyosin + H2O

degradation at pH 4.0

troponin I + H2O

degradation at pH 4.0, little degradation at pH 5.o-5.6

VALSLKISIGNVVKTMQFEPST + H2O

the peptide mimicks the N-terminus of the talin F0 head domain

732111

Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O

no substrate for wild type enzyme, endopeptidase activity of mutant T11_E18del-cathepsin H

650475

Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O

benzoyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

Fibronectin + H2O

Q86MW6

Gelatin + H2O

Q86MW6

Proteins + H2O

surfactant protein B + H2O

Mus musculus

718296

additional information

enzyme is involved in maturation of the biologically active surfactant protein B

669215

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide

1,10-phenanthroline

1,3,5-triphenyl pyrazole

1,3,5-triphenyl-2-pyrazoline

1H-spiro(cyclohexane-1,2-quinazolin)-4(3H)-one

competitive inhibition, 94.86% residual activity at 0.1 mM

2,2'-dipyridyl disulfide

2-(4-(dimethylamino)phenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 88.37% residual activity at 0.1 mM

2-(4-bromophenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 91.62% residual activity at 0.1 mM

2-(4-chlorophenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 98.11% residual activity at 0.1 mM

2-(4-fluorophenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 94.05% residual activity at 0.1 mM

2-(4-methoxyphenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 95.94% residual activity at 0.1 mM

2-(4-methylphenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 39.18% residual activity at 0.1 mM

2-(4-nitrophenyl)-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 81.89% residual activity at 0.1 mM

2-chlorobenzohydrazide

non-competitive inhibition; non-competitive inhibition, 89.46% residual activity at 0.01 mM

2-methoxybenzohydrazide

non-competitive inhibition; non-competitive inhibition, 97.83% residual activity at 0.01 mM

2-phenyl-2,3-dihydroquinazolin-4(1H)-one

competitive inhibition, 79.73% residual activity at 0.1 mM

3,5-diphenyl-2-pyrazoline

3,5-diphenyl-4-amino-1,2,4-triazole

non-competitive inhibition; non-competitive inhibition, 69.73% residual activity at 0.01 mM

3-(2'-hydroxy-3'-methylphenyl)-5-(2'-hydroxy-3'-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 88.11% residual activity at 0.01 mM

3-(2'-methylphenyl)-5-(2'-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 82.97% residual activity at 0.01 mM

3-(2-hydroxy-3-methylphenyl)-5-(2-hydroxy-3-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-(2-methylphenyl)-5-(2-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-(3'-aminophenyl)-5-(3'-aminophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 16.37% residual activity at 0.01 mM

3-(3'-methylphenyl)-5-(3'-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 79.45% residual activity at 0.01 mM

3-(3'-nitrophenyl)-5-(3'-nitrophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 45.13% residual activity at 0.01 mM

3-(3-aminophenyl)-5-(3-aminophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-(3-methylphenyl)-5-(3-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-(4'-chlorophenyl)-5-(4'-chlorophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 24.35% residual activity at 0.01 mM

3-(4'-methylphenyl)-5-(4'-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 89.46% residual activity at 0.01 mM

3-(4-chlorophenyl)-5-(4-chlorophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-(4-methylphenyl)-5-(4-methylphenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-phenyl-5-(3-nitrophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

3-phenyl-5-(4'-nitrophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition, 78.11% residual activity at 0.01 mM

3-phenyl-5-(4-nitrophenyl)-4-amino-1,2,4-triazole

non-competitive inhibition

4-aminophenylmercuric acetate

93% inhibition at 1 mM

4-bromochalcone phenyl hydrazone

4-chlorochalcone phenyl hydrazone

4-hydroxybenzohydrazide

non-competitive inhibition; non-competitive inhibition, 91.62% residual activity at 0.01 mM

4-methoxybenzohydrazide

non-competitive inhibition; non-competitive inhibition, 45% residual activity at 0.01 mM

4-methoxychalcone phenyl hydrazone

4-methylchalcone phenyl hydrazone

4-nitrochalcone phenyl hydrazone

5,5'-dithiobis(2-nitrobenzoic acid)

95% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine

5-(4-bromophenyl)-1,3-diphenyl pyrazole

5-(4-bromophenyl)-1,3-diphenyl-2-pyrazoline

5-(4-bromophenyl)-3-phenyl-2-pyrazoline

5-(4-chlorophenyl)-1,3-diphenyl pyrazole

5-(4-chlorophenyl)-1,3-diphenyl-2-pyrazoline

5-(4-chlorophenyl)-3-phenyl-2-pyrazoline

5-(4-methoxyphenyl)-1,3-diphenyl pyrazole

5-(4-methoxyphenyl)-1,3-diphenyl-2-pyrazoline

5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline

5-(4-methylphenyl)-1,3-diphenyl pyrazole

5-(4-methylphenyl)-1,3-diphenyl-2-pyrazoline

5-(4-methylphenyl)-3-phenyl-2-pyrazoline

5-(4-nitrophenyl)-1,3-diphenyl pyrazole

5-(4-nitrophenyl)-1,3-diphenyl-2-pyrazoline

5-(4-nitrophenyl)-3-phenyl-2-pyrazoline

acetyl-(Ala)2-Pro-Ala-CH2Cl

100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine

acetyl-(Ala)2-Pro-Val-CH2Cl

100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine

acetyl-(Ala)4-CH2Cl

100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine

alpha-2-Macroglobulin

antipain

benzyloxycarbonyl-Gly-Leu-NH

inhibits processing of proenzyme to active form

638817

Benzyloxycarbonyl-Leu-NH2

inhibits formation of active enzyme from procathepsin H

638817

Benzyloxycarbonyl-Phe-Phe-CHN2

31% inhibition at 0.01 mM

bestatin

62% inhibition at 1 mM, substrate Leu-2-N-naphthylamine

bovine stefin B

CdCl2

90% inhibition at 1 mM

chalcone phenyl hydrazone

chicken cystatin

chicken egg white cystatin

650475

chymostatin B

47% inhibition at 0.016 mM

CNWAAGYNCGGGS-NH2

cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain

CNWTLGGYKCGGGS-NH2

cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain

CoCl2

100% inhibition at 1 mM

CPI-H

peptide inhibitor of 13 kDa from rabbit skeletal muscle, noncompetitive

CPI-L

peptide inhibitor of 23 kDa from rabbit skeletal muscle, noncompetitive

CuCl2

94% inhibition at 1 mM

CWEWGGWHCGGSS-OH

cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain

CWSMMGFQCGGGS-NH2

cyclic peptide with intramolecular disulfide bond, comparison of inhibitory effect with cathepsin K, cathepsin L, cathepsin B and papain

cystatin

cystatin C

cystatin C, full-length

cystatin C, truncated form

deletion of 10 N-terminal amino acids

decyl bromide

100% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine

DTNB

100% inhibition at 1 mM

E-64

Mus musculus

irreversible active site inhibitor

709114

ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate

H2N-Ser(O-Bzl)-CHN2

HgCl2

Human cystatin C

Ki: 0.01-0.03 nM

human stefin A

human stefin B

Hyamine 1622

52% inhibition at 1 mM

iodoacetamide

iodoacetic acid

Leu-CH2-Cl

complete inhibition at 0.01 mM

Leu-CH2Cl

leupeptin

MnSO4

64% inhibition at 1 mM, substrate Leu-2-N-naphthylamine

N-acetyl-3,5-diphenyl pyrazolines

N-acetyl-5-(4-bromophenyl)-3-phenyl-2-pyrazoline

N-acetyl-5-(4-chlorophenyl)-3-phenyl-2-pyrazoline

N-acetyl-5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline

N-acetyl-5-(4-methylphenyl)-3-phenyl-2-pyrazoline

N-acetyl-5-(4-nitrophenyl)-3-phenyl-2-pyrazoline

N-benzoyl-3,5-diphenylpyrazoline

; 66.19% residual activity at 0.01 mM

N-benzoyl-5-(2-chloro phenyl)-3-phenylpyrazoline

N-benzoyl-5-(2-chlorophenyl)-3-phenylpyrazoline

55.13% residual activity at 0.01 mM

N-benzoyl-5-(2-methoxyphenyl)-3-phenylpyrazoline

; 79.37% residual activity at 0.01 mM

N-benzoyl-5-(2-nitrophenyl)-3-phenylpyrazoline

; 34.77% residual activity at 0.01 mM

N-benzoyl-5-(3-chloro phenyl)-3-phenylpyrazoline

N-benzoyl-5-(3-chlorophenyl)-3-phenylpyrazoline

44.2% residual activity at 0.01 mM

N-benzoyl-5-(3-methoxy phenyl)-3-phenylpyrazoline

N-benzoyl-5-(3-methoxyphenyl)-3-phenylpyrazoline

76.06% residual activity at 0.01 mM

N-benzoyl-5-(3-nitrophenyl)-3-phenylpyrazoline

; 27.49% residual activity at 0.01 mM

N-benzoyl-5-(4-chloro phenyl)-3-phenylpyrazoline

N-benzoyl-5-(4-chlorophenyl)-3-phenylpyrazoline

62.47% residual activity at 0.01 mM

N-benzoyl-5-(4-methoxy phenyl)-3-phenylpyrazoline

N-benzoyl-5-(4-methoxyphenyl)-3-phenylpyrazoline

69.69% residual activity at 0.01 mM

N-benzoyl-5-(4-nitrophenyl)-3-phenylpyrazoline

; 28.77% residual activity at 0.01 mM

N-ethylmaleimide

N-formyl-3,5-diphenylpyrazoline

; 76.78% residual activity at 10 mM

N-formyl-5-(2-chlorophenyl)-3-phenylpyrazoline

; 61.38% residual activity at 10 mM

N-formyl-5-(2-methoxyphenyl)-3-phenylpyrazoline

; 92.97% residual activity at 0.001 mM

N-formyl-5-(2-nitrophenyl)-3-phenylpyrazoline

; 30.2% residual activity at 0.001 mM

N-formyl-5-(3-chlorophenyl)-3-phenylpyrazoline

; 49.57% residual activity at 0.001 mM

N-formyl-5-(3-methoxyphenyl)-3-phenylpyrazoline

; 86.03% residual activity at 0.001 mM

N-formyl-5-(3-nitrophenyl)-3-phenylpyrazoline

; 14.15% residual activity at 0.001 mM

N-formyl-5-(4-chlorophenyl)-3-phenylpyrazoline

; 69.38% residual activity at 0.001 mM

N-formyl-5-(4-methoxyphenyl)-3-phenylpyrazoline

; 80.06% residual activity at 0.001 mM

N-formyl-5-(4-nitrophenyl)-3-phenylpyrazoline

; 27.64% residual activity at 0.001 mM

N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide

N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide

p-Hydroxymercuriphenylsulfonic acid

83% inhibition at 0.011 mM, substrate Leu-2-N-naphthylamine

PCMB

46% inhibition at 1 mM

phenylacetyl hydrazine

non-competitive inhibition; non-competitive inhibition, 81.08% residual activity at 0.01 mM

Proteinase inhibitor

puromycin

Soybean trypsin inhibitor

Thiol proteinase inhibitor

thiol-blocking agents

TLCK

tosyl-Leu-CH2Cl

tosyl-Phe-CH2Cl

TPCK

Zn(CH3COO)2

85% inhibition at 1 mM

ZnSO4

87% inhibition at 1 mM, substrate Leu-2-N-naphthylamine

additional information

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

2-mercaptoethanol

cysteine

dithioerythritol

activates, synergistic with EDTA

dithiothreitol

EDTA

glutathione

thioglycolic acid

activates, synergistic with EDTA

thiol compounds

additional information

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210

Ala-4-methylcoumaryl-7-amide

0.367

alpha-N-benzoyl-DL-Arg-beta-naphthylamide

pH 6.2, 37°C

0.097 - 0.169

Arg-2-naphthylamide

0.097 - 72

Arg-4-methylcoumaryl-7-amide

0.637

benzoyl-Arg-2-naphthylamide

0.321

benzoyl-Arg-4-methylcoumaryl-7-amide

at 30°C

0.039 - 0.05

benzoyl-DL-Arg-2-naphthylamide

0.46

benzoyl-L-Arg-2-naphthylamide

638826

0.082

benzoyl-L-Phe-L-Arg-4-methylcoumaryl-7-amide

pH 6.5, 25°C, mutant lacking the mini chain

0.025 - 0.038

benzoyl-L-Phe-L-Val-L-Arg-4-methylcoumaryl-7-amide

7.4

benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro

3.5

benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2

0.076

citrulline-4-methylcoumaryl-7-amide

at 30°C

0.05 - 3.6

H-L-Arg-NH-4-methylcoumaryl-7-amide

0.1

L-Arg-4-methylcoumaryl-7-amide

pH 6.5, 25°C, native enzyme

0.203

L-Leu-beta-naphthylamide

pH 6.2, 37°C

0.167 - 0.22

Leu-2-naphthylamide

35.4

Leu-4-methylcoumaryl-7-amide

184

Lys-4-methylcoumaryl-7-amide

0.128

Met-2-naphthylamide

10.2

succinyl-Ala-Ala-4-nitroanilide

succinyl-Ala-Ala-Ala-4-nitroanilide

0.7

succinyl-Ala-Ala-Ala-Ala-4-nitroanilide

0.7

succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide

27.8

succinyl-Ala-Ala-Pro-4-nitroanilide

succinyl-Ala-Pro-Ala-4-nitroanilide

2.9

succinyl-Pro-Ala-Ala-4-nitroanilide

0.092

Z-L-Phe-L-Arg-NH-4-methylcoumaryl-7-amide

mutant T11_E18del-cathepsin H, pH 6.8, 30°C

650475

additional information

pH and temperature dependence of kcat/Km

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

1.87

Ala-4-methylcoumaryl-7-amide

1.82 - 115

Arg-2-naphthylamide

2.2 - 8.13

Arg-4-methylcoumaryl-7-amide

1.82

benzoyl-Arg-2-naphthylamide

0.63

benzoyl-Arg-4-methylcoumaryl-7-amide

at 30°C

5.3

benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro

7.7

benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2

3.1

citrulline-4-methylcoumaryl-7-amide

at 30°C

122

Leu-2-naphthylamide

1.1

Leu-4-methylcoumaryl-7-amide

4.13

Lys-4-methylcoumaryl-7-amide

219

Met-2-naphthylamide

0.07

succinyl-Ala-Ala-4-nitroanilide

0.3

succinyl-Ala-Ala-Ala-4-nitroanilide

0.9

succinyl-Ala-Ala-Ala-Ala-4-nitroanilide

0.8

succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide

0.004

succinyl-Ala-Ala-Pro-4-nitroanilide

0.15

succinyl-Pro-Ala-Ala-4-nitroanilide

additional information

pH and temperature dependence of kcat/Km

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

0.0037

(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide

0.01859

1,3,5-triphenyl pyrazole

pH 7, 37°C

0.005388

1,3,5-triphenyl-2-pyrazoline

pH 7, 37°C

0.09009

1H-spiro(cyclohexane-1,2-quinazolin)-4(3H)-one

at pH 7.0 and 37°C

0.01858

2-(4-(dimethylamino)phenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.05263

2-(4-bromophenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.02325

2-(4-chlorophenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.00818

2-(4-fluorophenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.05682

2-(4-methoxyphenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.0025

2-(4-methylphenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.01042

2-(4-nitrophenyl)-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.0901

2-chlorobenzohydrazide

at pH 7.0 and 37°C; pH 5, 37°C

0.588

2-methoxybenzohydrazide

at pH 7.0 and 37°C; pH 5, 37°C

0.01441

2-phenyl-2,3-dihydroquinazolin-4(1H)-one

at pH 7.0 and 37°C

0.006024

3,5-diphenyl-2-pyrazoline

pH 7, 37°C

0.0232

3,5-diphenyl-4-amino-1,2,4-triazole

at pH 7.0 and 37°C; pH 5, 37°C

0.0781

3-(2'-hydroxy-3'-methylphenyl)-5-(2'-hydroxy-3'-methylphenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0532

3-(2'-methylphenyl)-5-(2'-methylphenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0781

3-(2-hydroxy-3-methylphenyl)-5-(2-hydroxy-3-methylphenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0532

3-(2-methylphenyl)-5-(2-methylphenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.002

3-(3'-aminophenyl)-5-(3'-aminophenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0387

3-(3'-methylphenyl)-5-(3'-methylphenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0086

3-(3'-nitrophenyl)-5-(3'-nitrophenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.002

3-(3-aminophenyl)-5-(3-aminophenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0387

3-(3-methylphenyl)-5-(3-methylphenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0033

3-(4'-chlorophenyl)-5-(4'-chlorophenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0901

3-(4'-methylphenyl)-5-(4'-methylphenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0033

3-(4-chlorophenyl)-5-(4-chlorophenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0901

3-(4-methylphenyl)-5-(4-methylphenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0086

3-phenyl-5-(3-nitrophenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0364

3-phenyl-5-(4'-nitrophenyl)-4-amino-1,2,4-triazole

at pH 7.0 and 37°C

0.0364

3-phenyl-5-(4-nitrophenyl)-4-amino-1,2,4-triazole

pH 5, 37°C

0.0026

4-bromochalcone phenyl hydrazone

pH 7, 37°C

0.0011

4-chlorochalcone phenyl hydrazone

pH 7, 37°C

0.1123

4-hydroxybenzohydrazide

at pH 7.0 and 37°C; pH 5, 37°C

0.0082

4-methoxybenzohydrazide

at pH 7.0 and 37°C; pH 5, 37°C

0.001695

4-methoxychalcone phenyl hydrazone

pH 7, 37°C

0.0029

4-methylchalcone phenyl hydrazone

pH 7, 37°C

0.001905

4-nitrochalcone phenyl hydrazone

pH 7, 37°C

0.0038

5-(4-bromophenyl)-1,3-diphenyl pyrazole

pH 7, 37°C

0.002907

5-(4-bromophenyl)-1,3-diphenyl-2-pyrazoline

pH 7, 37°C

0.00537

5-(4-bromophenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.002346

5-(4-chlorophenyl)-1,3-diphenyl pyrazole

pH 7, 37°C

0.001174

5-(4-chlorophenyl)-1,3-diphenyl-2-pyrazoline

pH 7, 37°C

0.001568

5-(4-chlorophenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.0033

5-(4-methoxyphenyl)-1,3-diphenyl pyrazole

pH 7, 37°C

0.002119

5-(4-methoxyphenyl)-1,3-diphenyl-2-pyrazoline

pH 7, 37°C

0.0098

5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.007396

5-(4-methylphenyl)-1,3-diphenyl pyrazole

pH 7, 37°C

0.004184

5-(4-methylphenyl)-1,3-diphenyl-2-pyrazoline

pH 7, 37°C

0.0082

5-(4-methylphenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.00257

5-(4-nitrophenyl)-1,3-diphenyl pyrazole

pH 7, 37°C

0.003158

5-(4-nitrophenyl)-1,3-diphenyl-2-pyrazoline

pH 7, 37°C

0.0033

5-(4-nitrophenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.00000052

bovine stefin B

pH 6.5, 25°C, native enzyme

0.0048

chalcone phenyl hydrazone

pH 7, 37°C

0.000000049

chicken cystatin

pH 6.5, 25°C, mutant lacking the mini chain

0.000000095 - 0.00000053

chicken egg white cystatin

0.0179

CNWAAGYNCGGGS-NH2

pH 6.8, 22°C

0.0051

CNWTLGGYKCGGGS-NH2

pH 6.8, 22°C

0.00000033

CPI-H

pH 6.2, 30°C

0.0000005

CPI-L

pH 6.2, 30°C

0.0049

CWEWGGWHCGGSS-OH

pH 6.8, 22°C

0.0039

CWSMMGFQCGGGS-NH2

pH 6.8, 22°C

0.000000071

cystatin C, full-length

25°C, pH 7.0

0.00000032

cystatin C, truncated form

25°C, pH 7.0

0.000000099

human stefin A

pH 6.5, 25°C, mutant lacking the mini chain

0.000000074

human stefin B

pH 6.5, 25°C, mutant lacking the mini chain

0.005

leupeptin

0.01767

N-acetyl-3,5-diphenyl pyrazolines

pH 7, 37°C

0.01266

N-acetyl-5-(4-bromophenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.01

N-acetyl-5-(4-chlorophenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.14

N-acetyl-5-(4-methoxyphenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.03546

N-acetyl-5-(4-methylphenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.02432

N-acetyl-5-(4-nitrophenyl)-3-phenyl-2-pyrazoline

pH 7, 37°C

0.0032

N-benzoyl-3,5-diphenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.00189

N-benzoyl-5-(2-chloro phenyl)-3-phenylpyrazoline

pH 7, 37°C

0.00189

N-benzoyl-5-(2-chlorophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C

0.00965

N-benzoyl-5-(2-methoxyphenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.00144

N-benzoyl-5-(2-nitrophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.00181

N-benzoyl-5-(3-chloro phenyl)-3-phenylpyrazoline

pH 7, 37°C

0.00181

N-benzoyl-5-(3-chlorophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C

0.00503

N-benzoyl-5-(3-methoxy phenyl)-3-phenylpyrazoline

pH 7, 37°C

0.00503

N-benzoyl-5-(3-methoxyphenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C

0.00098

N-benzoyl-5-(3-nitrophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.00301

N-benzoyl-5-(4-chloro phenyl)-3-phenylpyrazoline

pH 7, 37°C

0.00301

N-benzoyl-5-(4-chlorophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C

0.0041

N-benzoyl-5-(4-methoxy phenyl)-3-phenylpyrazoline

pH 7, 37°C

0.0041

N-benzoyl-5-(4-methoxyphenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C

0.00127

N-benzoyl-5-(4-nitrophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000174

N-formyl-3,5-diphenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000135

N-formyl-5-(2-chlorophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000387

N-formyl-5-(2-methoxyphenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.0000963

N-formyl-5-(2-nitrophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.00013

N-formyl-5-(3-chlorophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000272

N-formyl-5-(3-methoxyphenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.0000519

N-formyl-5-(3-nitrophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000137

N-formyl-5-(4-chlorophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000221

N-formyl-5-(4-methoxyphenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.000058

N-formyl-5-(4-nitrophenyl)-3-phenylpyrazoline

in 0.1 M acetate buffer, at pH 5.0 and 37°C; pH 7, 37°C

0.00014

N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide

0.0014

N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide

0.0425

phenylacetyl hydrazine

at pH 7.0 and 37°C; pH 5, 37°C

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0.000109

0.0088

pH 6.2, 37°C

0.02256

0.02291

pH 7, 37°C

0.15

substrate benzyloxycarbonyl-Arg-Arg-2-naphthylamide

1.73

substrate benzoyl-DL-Arg-2-naphthylamide

1.95

substrate Leu-2-naphthylamide

3.5

substrate Arg-2-naphthylamide

7.45

7.714

29.7

286

additional information

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assay at

5 - 6

5.5

6.5 - 7.2

substrate-dependent

6.5

substrate Arg-4-methylcoumaryl-7-amide

6.5 - 7.2

substrate-dependent

6.5

6.6

substrate alpha-N-benzoyl-DL-Arg-beta-naphthylamide

6.8

7.2

substrate Leu-2-naphthylamide

7.5

isoform 2

or higher, substrate benzoyl-Arg-4-methylcoumaryl-7-amide

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5 - 8

less than 20% of maximal activity above and below

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substrate Arg-4-methylcoumaryl-7-amide

45 - 50

substrate-dependent

substrate benzoyl-Arg 2-naphthylamide

638833

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6.3 - 6.8

and minor band at 6.1, isoelectric focusing

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adrenal medulla

adrenal medullary chromaffin cell

732360

basal cell carcinoma cell

bronchoalveolar lavage fluid

high expression level in intestine and gill, but barely in other tissues

cultured

high expression level in intestine and gill, but barely in other tissues

cathepsin B3 is detected in the whole body extract of metacercariae and newly excysted juveniles but not in 4-week-old juveniles or adult parasites

musculus semimembranosus

cathepsin H is highly expressed in the secretory organelles of alveolar type II pneumocytes

inflammated

683 ng /mg protein, distribution in tissues and peripheral blood cells

additional information

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6czk, download, 3D-view

6czs, download, 3D-view

1nb3, download, 3D-view

1nb5, download, 3D-view

8pch, download, 3D-view

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17000

1 * 17000, 1 * 5000, gel filtration after reduction

22000

23000

gel filtration

24000

1 * 24000, 1 * 4000, SDS-PAGE, partial

638816

25000

25100

estimated from sequence of cDNA

26000

27900

mature enzyme, calculated from amino acid sequence

28000

29000

gel filtration

30000

34000

native cathepsin B3, SDS-PAGE

35000

procathepsin H, estimated from sequence of cDNA

638820

36600

Ictalurus punctatus

B5LVX3

x * 36600, calculated

698021

37000

37100

preprocathepsin H, estimated from sequence of cDNA

638820

38000 - 39000

deglycosylation of recombinant CatB3 with endoglycosidase H under denaturing and native conditions produces protein migrating at 38000-39000 Da

39200

proenzyme, calculated from amino acid sequence

55000 - 75000

purified recombinant glycosylated proCatB3 forms migrate at approximately 55000-75000Da

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dimer

monomer

8 entries

additional information

8 entries

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glycoprotein

proteolytic modification

side-chain modification

7 entries

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comparison of structure and primary sequences of 5 papain, actinidin, cathepsins B and H and stem bromelain yields high degree of similarity

general and secondary structure, structure of active-site cleft, mechanistic conclusions drawn from crystallographic study, mini-chain attached to active-site cleft of enzyme body via disulfide bond, structural comparison reveals high similarity to actinidin

638836

in complex with human stefin A

652906

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4 - 7.5

638838

5 - 9

638840

5.5 - 6

rapid inactivation above

638839

irreversible inactivation above

36634, 638831

8.2

stable at 25°C, unstable at 37°C

638825

8.5

83% activity

638833

additional information

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rapid inactivation above, without substrate bound

30 min, 80% loss of activity

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effects of endoglycosidase H on proenzyme stability

638819

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-15°C, goat muscle, 120 days, remains stable with 84-94% residual activity

Capra aegagrus

681150

-20°C, 0.02 M sodium acetate buffer, pH 5.2, 1 mM EDTA, several months without loss of activity

638816

4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA

4°C or -20°C, 50-100 mM Na-acetate buffer, pH 5.5, 1 mM Na2-EDTA, 0.1% Brij 35, 5 mM Na-tetrathionate, 1 month, 0-10% loss of activity

4°C, 0.02 M Na-acetate buffer, pH 5.0, 0.15 mM NaCl

638825

4°C, 50 mM Na acetate buffer, pH 4.6, 1 mM EDTA, 1 month, concentrated preparations, no loss of activity

638833

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2 methods

ammonium sulfate precipitation, CM Sephadex column chromatography, C-5 column chromatography, DEAE Sephadex A-5 column chromatography, and Sephadex G-1 and gel filtration

ammonium sulfate precipitation, CM-Sephadex column chromatography, C-50 column chromatography, DEAE-Sephadex A-50., and Sephadex G-100, and gel filtration

Ni2+-NTA column chromatography