Application | Comment | Organism |
---|---|---|
drug development | cathepsin B could be a drug target for several pathologies, based mainly on its role in cancer progression and osteoarthritis in humans | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in inclusion bodies in Escherichia coli | Homo sapiens |
expression in inclusion bodies in Escherichia coli, cathepsin B gene is located on chromosome 14 of the mouse genome | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of cathepsin B transgenic mouse models for investigation of human pathologies | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CA-074 | irreversible cathepsin B inhibitor | Homo sapiens | |
CA-074 | irreversible cathepsin B inhibitor | Mus musculus | |
chicken cystatin | - |
Homo sapiens | |
chicken cystatin | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Mus musculus | |
0.075 | - |
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Homo sapiens | |
0.212 | - |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Homo sapiens | |
0.226 | - |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | disturbances in the regulation of the intracellular activities and uncontrolled release of cathepsins from lysosomes have been implicated in many disease states, such as osteoporosis, inflammatory diseases, and tumor progression | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07858 | - |
- |
Mus musculus | P10605 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant renatured enzyme expressed in inclusion bodies in Escherichia coli | Homo sapiens |
recombinant renatured enzyme expressed in inclusion bodies in Escherichia coli | Mus musculus |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies from Escherichia coli | Homo sapiens |
recombinant enzyme from inclusion bodies from Escherichia coli | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | embryonic | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | disturbances in the regulation of the intracellular activities and uncontrolled release of cathepsins from lysosomes have been implicated in many disease states, such as osteoporosis, inflammatory diseases, and tumor progression | Homo sapiens | ? | - |
? | |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Nalpha-benzoyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | Nalpha-benzoyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure comparison to the murine enzyme | Homo sapiens |
More | three-dimensional enzyme structure, modeling, structure comparison to the human enzyme, overview | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the group of cysteine proteases | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Mus musculus | |
67 | - |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Homo sapiens | |
86 | - |
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Mus musculus | |
115 | - |
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin | pH 6.0, 37°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Homo sapiens |
6 | - |
assay at | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000003 | - |
chicken cystatin | - |
Homo sapiens | |
0.00000125 | - |
chicken cystatin | - |
Mus musculus |