Literature summary for 3.4.22.1 extracted from
Sharma, S.; Mittal, A.; Gupta, V.K.; Singh, H.
Improved stabilization of microencapsulated cathepsin B in harsh conditions (2007), Enzyme Microb. Technol., 40, 337-342.
No PubMed abstract available
General Stability
General Stability |
Organism |
CM-cellulose and 1% CaCl2 concentration for cationic solution and 3% sodium alginate concentration for anionic solution, the encapsulated enzyme can retain about 60-70% of the immobilized activity even after five batch reactions whereas at other concentrations of CM-cellulose (2.0-3.0%), CaCl2 (1.5-2.5%) and sodium alginate (1.0-2.0%), the retention of enzyme activity is less (about 28-50%) |
Capra aegagrus |
the encapsulated form of enzyme can retain about 60% of its original activity during its reusability for more than five successive batch reactions, the percent-encapsulated activity is about 53% at 4% CM-cellulose concentration, at 4% |
Capra aegagrus |
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
1.02 |
- |
alpha-N-benzoyl-DL-Arg-beta-naphthylamide |
free enzyme |
Capra aegagrus |
|
1.5 |
2 |
alpha-N-benzoyl-DL-Arg-beta-naphthylamide |
encapsulated enzyme |
Capra aegagrus |
|
Organic Solvent Stability
Organic Solvent |
Comment |
Organism |
Ethanol |
at 15% (v/v) ethanol immobilized enzyme loses about 40% of its original activity whereas the free form loses almost all the enzyme activity |
Capra aegagrus |
Organism
Organism |
UniProt |
Comment |
Textmining |
Capra aegagrus |
- |
Capra aegagrus hircus |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
ammonium sulfate fractionation, Sephadex G-100 gel filtration, organomercurial affinity chromatography, and CM-Sephadex C-50 gel filtration |
Capra aegagrus |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
brain |
- |
Capra aegagrus |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
alpha-N-benzoyl-DL-Arg-beta-naphthylamide + H2O |
- |
Capra aegagrus |
alpha-N-benzoyl-DL-Arg + beta-naphthylamine |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
CatB |
- |
Capra aegagrus |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
50 |
- |
free enzyme |
Capra aegagrus |
55 |
- |
encapsulated enzyme |
Capra aegagrus |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
50 |
70 |
the free enzyme has a half-life of 141.7 min at 50°C and 12.9 min at 70°C, the encapsulated enzyme has a half-life of 1559.2 min at 50°C and 20.8 min at 70°C, the free enzyme loses almost whole of its activity when treated at 60°C for 45 min while the immobilized retains about 25% of its original activity |
Capra aegagrus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
6 |
6.5 |
encapsulated enzyme |
Capra aegagrus |
6 |
- |
free enzyme |
Capra aegagrus |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
3.5 |
7.5 |
encapsulated enzyme |
Capra aegagrus |
4 |
6.5 |
free enzyme |
Capra aegagrus |