Cloned (Comment) | Organism |
---|---|
pcDNA3 plasmid encoding either PC2 wild-type or mutant S383A expressed in CHO-K1/7B2 cells or AtT-20/7B2 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
S383A | active site mutant, seems to be folded correctly. Is efficiently secreted as the intact zymogen in CHO-K1 cells. Its propeptide can productively insert into the mutated binding pocket without causing misfolding. In AtT-20 cells, mutant S383A is cleaved at the secondary cleavage site within the propeptide. The mutant can not be cleaved by active PC2, so that other proprotein convertases, but not PC2, may be responsible for secondary site processing. This cleavage event is pH-dependent and is inhibited by the proprotein convertase inhibitor decanoyl-Arg-Val-Lys-Arg-chloromethylketone | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
decanoyl-Arg-Val-Lys-Arg-chloromethylketone | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PC2 wild-type and mutant S383A purified on anion-exchange column and by gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
proPC2 + H2O | - |
Mus musculus | mature PC2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PC2 | - |
Mus musculus |
prohormone convertase 2 | - |
Mus musculus |