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Literature summary for 3.4.21.53 extracted from

  • Lee, J.H.; Ancona, V.; Zhao, Y.
    Lon protease modulates virulence traits in Erwinia amylovora by direct monitoring of major regulators and indirectly through the Rcs and Gac-Csr regulatory systems (2018), Mol. Plant Pathol., 19, 827-840 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.53

Protein Variants

Protein Variants Comment Organism
additional information generation of an insertional mutant strain with a defect in the lon gene in the background of the wild-type strain Ea1189. The Ea1189 lon mutant exhibits a mucoid colony on growth medium and produces about 10 times more amylovoran than that of the wild-type strain, which can be partially complemented. The Ea1189 lon mutant induces a typical hypersensitive response lesion on tobacco and is as pathogenic as wild-type on immature pears, although the disease progress is similar or slightly faster in the mutant at 4 days post-inoculation Erwinia amylovora

Organism

Organism UniProt Comment Textmining
Erwinia amylovora P46067
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Erwinia amylovora Ea1189 P46067
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Synonyms

Synonyms Comment Organism
lon
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 Erwinia amylovora
lon protease
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 Erwinia amylovora

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Erwinia amylovora

Expression

Organism Comment Expression
Erwinia amylovora gene lon expression is under the control of CsrA, possibly at both the transcriptional and post-transcriptional levels. Transcription of lon is suppressed by CsrA down

General Information

General Information Comment Organism
malfunction mutation of the lon gene leads to the overproduction of amylovoran, increased T3SS gene expression and the nonmotile phenotype. Erwinia amylovora depends on the type III secretion system (T3SS) and the exopolysaccharide (EPS) amylovoran to cause disease, and deletion of the lon gene in the csrA mutant only rescues amylovoran production, but not T3SS. RcsA/RcsB accumulation suppresses motility and flhD transcription in the lon mutant. Expression of the csrB sRNA is suppressed by RcsA/RcsB accumulation in the lon mutant Erwinia amylovora
metabolism a connection between Lon and the GacS/GacACsr regulatory system might exist Erwinia amylovora
physiological function Lon is a highly conserved cytosolic protease belonging to the AAA+ superfamily of ATPase, and acts as a major player in general protein quality control by degrading damaged or misfolded proteins. The proteolytic activity of Lon also contributes to the post-translational regulation of functional proteins. molecular mechanisms underlying Lon-mediated virulence regulation in Erwinia amylovora, an enterobacterial pathogen of apple. Gene lon expression is under the control of CsrA, possibly at both the transcriptional and post-transcriptional levels. CsrA might positively control both T3SS and amylovoran production partly by suppressing Lon. Lon negatively regulates amylovoran by targeting RcsA, but not RcsB. Lon-dependent degradation of RcsA regulates the expression of hrpS in Erwinia amylovora. Lon is essential for motility in Erwinia amylovora Erwinia amylovora