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Literature summary for 3.4.21.42 extracted from
- Human complement serine proteases C1r and C1s and their proenzymes (1993), Methods Enzymol., 223, 61-82.
General Stability
| General Stability | Organism |
|---|---|
| no autolytic cleavage | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| C1bar inhibitor | - |
Homo sapiens | |
| DFP | - |
Homo sapiens |  |
| p-nitrophenyl-p'-guanidinobenzoate | - |
Homo sapiens | |
| PMSF | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | 0.07 | N-benzyloxycarbonyl-Tyr p-nitrophenyl ester | - |
Homo sapiens | |
| 0.069 | - |
N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester | - |
Homo sapiens | |
| 1.7 | 2.8 | tosyl-Arg methyl ester | - |
Homo sapiens | |
| 2.5 | 5 | benzoyl-Arg ethyl ester | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78900 | - |
x * 78900, monomer protein that forms Ca2+-dependent dimers, calculation from amino acid sequence and carbohydrate content | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | activation occurs through cleavage of a single Arg-Ile bond by C1rbar that yields active proteases comprising two chains linked by a single disulfide bride | Homo sapiens |
| proteolytic modification | C1s is the proenzyme of C1sbar | Homo sapiens |
| side-chain modification | glycoprotein | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| C1s and C1sbar | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-Arg ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| N-acetyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| N-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| N-benzyloxycarbonyl-Tyr-p-nitrophenyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| Nalpha-acetyl-Tyr ethyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| Nalpha-tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
| Tosyl-Arg methyl ester + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78900, monomer protein that forms Ca2+-dependent dimers, calculation from amino acid sequence and carbohydrate content | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | - |
10 min, 90% loss of activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | irreversible inactivation below pH 5.0 and above pH 9.5 | Homo sapiens |
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