Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose 6 column chromatography and HiTrap-CM column chromatography | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-casein + H2O | the enzyme is highly specific and hydrolyses the peptide bond predominantly on the carboxy side of Glu residues while hydrolysis on the carboxyl side of Asp residues is also observed. Hydrolysis does not occur while Pro is at the P1' position. In Glu-Glu-X (X equals Arg, Asn, Ile and Ser) and Glu-Glu-Glu-Lys sequences, hydrolysis of Glu-X and Glu-Lys is preferred | Bacillus licheniformis | ? | - |
? | |
N-acetyl-L-Glu-4-nitroanilide + H2O | - |
Bacillus licheniformis | N-acetyl-L-Glu + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alcalase | commercial preparation containing subtilisin and glutamyl endopeptidase activities | Bacillus licheniformis |