Information on EC 3.4.21.19 - glutamyl endopeptidase

Staphylococcus aureus NCTC 8325

Q2FZL2

753799

V8 protease

Q2FZL2

V8 protease

Staphylococcus aureus Wood 46

V8 protease

V8 proteinase

709024, 710230

V8-GSE

V8-GSE

VSPase

Staphylococcus aureus C-66

717680

VSPase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

Preferential cleavage: Glu-/-, Asp-/-

8 entries

Preferential cleavage: Glu-/-, Asp-/-

absolutely specific for cleavage sites Glu-/-Xaa and Asp-/-Xaa, cleavage exclusively at the carboxylic side, active site Ser169

649239

Preferential cleavage: Glu-/-, Asp-/-

catalytic tetrad of His213, Asp102, Ser195, and Trp214, computer modeling of S1 and S'1 sites of the enzyme's active site

Preferential cleavage: Glu-/-, Asp-/-

catalytic triad of His, Asp, and Ser

Bacillus subtilis

Preferential cleavage: Glu-/-, Asp-/-

catalytic triad of His, Asp, and Ser, computer modeling of active site structure, S1-substrate binding site, and substrate binding mechanism

Preferential cleavage: Glu-/-, Asp-/-

catalytic triad of His, Asp, and Ser, substrate binding mechanism and structure

Streptomyces griseus

Preferential cleavage: Glu-/-, Asp-/-

specific for cleavage on the carboxylic site of glutamic acid, catalytic site are His47 and Ser171, the N-terminus is involved in formation of the substrate binding pocket, a direct structural relation between zymogen activation and substrate charge compensation exists

650241

Preferential cleavage: Glu-/-, Asp-/-

catalytic tetrad of His213, Asp102, Ser195, and Trp214, computer modeling of S1 and S'1 sites of the enzyme's active site

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

condensation

formation of peptide bond

hydrolysis of peptide bond

endopeptidase

formation of peptide bond

formation of peptide bond

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CAS REGISTRY NUMBER

COMMENTARY

137010-42-5

82062-91-7

formerly

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

22 kDa human growth hormone + H2O

peptide fragment 1-32 of 22 kDa human growth hormone + peptide fragment 33-191 of 22 kDa human growth hormone

V8-protease digestion generates the fragment amino acids 33-191, resulting from a cleavage of the amino acids 32-33 bond

708626

6-azido-4-(4-iodophenethylamino)quinazoline-labeled 49 kDa subunit of NADH-ubiquinone oxidoreductase + H2O

proteolytic mapping of the 49 kDa subunit with V8-protease, cleavage within the sequence region Asp41-Arg63: fragment A is predicted to be the peptide Thr25-Glu248, 224 amino acids, 26.0 kDa, which is further cleaved at Glu143 and give fragment B, Thr25-Glu143, 118 amino acids, overview

707505

Abz-Ala-Phe-Ala-Phe-Glu-Val-Phe-(NO2)-Tyr-Asp + H2O

Abz-Ala-Phe-Ala-Phe-Glu + Val-Phe-(NO2)-Tyr-Asp

81321

Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O

acetyl-Asp-p-nitroanilide + H2O

acetyl-Glu + p-nitroaniline

acetyl-Glu-4-nitrophenyl + H2O

acetyl-Glu + 4-nitrophenol

acetyl-Glu-4-nitrophenyl ester + H2O

acetyl-Glu + 4-nitrophenol

acetyl-Glu-p-nitroanilide + H2O

acetyl-Glu + p-nitroaniline

Ala-Glu-4-methylcoumaryl-7-amide + H2O

alpha-casein + H2O

alpha1-antitrypsin + H2O

6-bromomethyl-2-(2-furanyl)-3-hydroxychromone-labeled substrate, V8 proteinase-induced cleavage of the reactive center loop does not generate any significant change in the Cys-232 region, but inactivates the anti-PPE property of the substrate, interaction analysis, overview

710230

Arg-Lys-Asp-Val-Tyr + H2O

Arg-Lys-Asp + Val-Tyr

Arg-Lys-Glu-Val-Tyr + H2O

Arg-Lys-Glu + Val-Tyr

azocasein + H2O

benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O

benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O

benzyloxycarbonyl-Ala + 2-carboxyphenylthiol

benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O

benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O

benzyloxycarbonyl-Ala + 3-carboxyphenol

benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O

benzyloxycarbonyl-Ala + 3-hydroxybenzoate

benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O

benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O

benzyloxycarbonyl-Ala + 4-carboxyphenol

benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O

benzyloxycarbonyl-Ala + 4-hydroxybenzoate

benzyloxycarbonyl-Ala carboxyethylthioester + H2O

benzyloxycarbonyl-Ala carboxyethylthioester + H2O

benzyloxycarbonyl-Ala + carboxyethylthiol

benzyloxycarbonyl-Ala carboxymethylthioester + H2O

benzyloxycarbonyl-Ala carboxymethylthioester + H2O

benzyloxycarbonyl-Ala + carboxymethylthiol

benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Glu-methyl ester + leucine * HCl

benzyloxycarbonyl-Ala-Ala-Glu-Leu + methanol

peptide synthesis

benzyloxycarbonyl-Ala-Ala-Leu-Asp-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Leu-Asp + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Leu-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Met-Asp-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Met-Asp + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Met-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Met-Glu + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Phe-Asp-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Phe-Asp + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Phe-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Trp-Asp-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Trp-Asp + 4-nitroaniline

benzyloxycarbonyl-Ala-Ala-Trp-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Trp-Glu + 4-nitroaniline

benzyloxycarbonyl-Ala-Glu-methyl ester + leucine * HCl

benzyloxycarbonyl-Ala-Glu-Leu + methanol

peptide synthesis

benzyloxycarbonyl-Ala-Glu-p-nitroanilide + H2O

benzyloxycarbonyl-Ala-Glu + p-nitroaniline

benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide + H2O

benzyloxycarbonyl-Ala-Leu-Glu + p-nitroaniline

benzyloxycarbonyl-Asp methyl ester + H2O

benzyloxycarbonyl-Asp + methanol

benzyloxycarbonyl-Asp-methyl ester + Ala-Ala-D-hydroxyalanine

benzyloxycarbonyl-Asp-Ala-Ala-D-hydroxyalanine + methanol

peptide synthesis, low activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Ala-Ala-hydroxyproline

benzyloxycarbonyl-Asp-Ala-Ala-hydroxyproline + methanol

peptide synthesis, lower activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Ala-D-hydroxyalanine

benzyloxycarbonyl-Asp-Ala-D-hydroxyalanine + methanol

peptide synthesis, very low activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyaspartate

benzyloxycarbonyl-Asp-Ala-hydroxyaspartate + methanol

peptide synthesis, low activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyglutamate

benzyloxycarbonyl-Asp-Ala-hydroxyglutamate + methanol

peptide synthesis, very low activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyproline

benzyloxycarbonyl-Asp-Ala-hydroxyproline + methanol

peptide synthesis, no activity at 25°C, only low activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Asp-Gly

benzyloxycarbonyl-Asp-Asp-Gly + methanol

peptide synthesis, no activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + aspartate

benzyloxycarbonyl-Asp-Asp + methanol

peptide synthesis, no activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + Glu-Gly

benzyloxycarbonyl-Asp-Glu-Gly + methanol

peptide synthesis, no activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Asp-methyl ester + glutamate

benzyloxycarbonyl-Asp-Glu + methanol

peptide synthesis, no activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Glu methyl ester + H2O

benzyloxycarbonyl-Glu + methanol

benzyloxycarbonyl-Glu methylthioester + H2O

benzyloxycarbonyl-Glu + methylthiol

benzyloxycarbonyl-Glu methylthioester + H2O

benzyloxycarbonyl-L-Glu + methylthiol

benzyloxycarbonyl-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Glu + 4-nitroaniline

benzyloxycarbonyl-Glu-methyl ester + Ala-Ala-D-Ala

benzyloxycarbonyl-Glu-Ala-Ala-D-Ala + methanol

peptide synthesis, low activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Ala-Ala-Pro

benzyloxycarbonyl-Glu-Ala-Ala-Pro + methanol

peptide synthesis, lower activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Ala-Asp

benzyloxycarbonyl-Glu-Ala-Asp + methanol

peptide synthesis, no activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Ala-D-Ala

benzyloxycarbonyl-Glu-Ala-D-Ala + methanol

peptide synthesis, no activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Ala-Glu

benzyloxycarbonyl-Glu-Ala-Glu + methanol

peptide synthesis, no activity at 25°C, but high activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Ala-Pro

benzyloxycarbonyl-Glu-Ala-Pro + methanol

peptide synthesis, no activity at 25°C, only low activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Asp-Gly

benzyloxycarbonyl-Glu-Asp-Gly + methanol

peptide synthesis, no activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + Glu-Gly

benzyloxycarbonyl-Glu-Glu-Gly + methanol

peptide synthesis, no activity at 25°C, but low activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + L-aspartate

benzyloxycarbonyl-Glu-Asp + methanol

peptide synthesis, no activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + L-glutamate

benzyloxycarbonyl-Glu-Glu + methanol

peptide synthesis, no activity at 25°C, but moderate activity at -15°C

benzyloxycarbonyl-Glu-methyl ester + L-tryptophan

benzyloxycarbonyl-Glu-Trp + methanol

peptide synthesis

benzyloxycarbonyl-Glu-methyl ester + Leu-Gly

benzyloxycarbonyl-Glu-Leu-Gly + methanol

peptide synthesis

benzyloxycarbonyl-Glu-methyl ester + leucine

benzyloxycarbonyl-Glu-Leu + methanol

peptide synthesis

benzyloxycarbonyl-Glu-methyl ester + Phe-Gly

benzyloxycarbonyl-Glu-Phe-Gly + methanol

peptide synthesis

benzyloxycarbonyl-Glu-p-nitroanilide + H2O

benzyloxycarbonyl-Glu + p-nitroaniline

6 entries

benzyloxycarbonyl-Gly-Ala-Ala-Asp-4-nitroanilide + H2O

benzyloxycarbonyl-Gly-Ala-Ala-Asp + 4-nitroaniline

benzyloxycarbonyl-Gly-Ala-Ala-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-Gly-Ala-Ala-Glu + 4-nitroaniline

benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide + H2O

benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu + 4-nitroaniline

670744

benzyloxycarbonyl-Leu-Glu-p-nitroanilide + H2O

benzyloxycarbonyl-Leu-Glu + p-nitroaniline

benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide + H2O

benzyloxycarbonyl-Leu-Leu-Glu + p-nitroaniline

benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O

benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline

benzyloxycarbonyl-Pro-Leu-Gly-S-CH2-COOH + LAFARAEAFG

benzyloxycarbonyl-PLGLAFARAEAFG + HS-CH2-COOH

acylation of peptide fragment by substrate mimetic

product formation 55%

benzyloxycarbonyl-S-CH2-COOH + LAFARAEAF-hydroxyglycine

benzyloxycarbonyl-LAFARAEAF-hydroxyglycine + HS-CH2-COOH

acylation of peptide fragment by substrate mimetic

product formation 99%

beta-casein + H2O

beta-type parvalbumin + H2O

peptide fragments

from the frog Rana catesbeiana

mass spectrometry for identification

bovine hemoglobin + H2O

peptide fragments

in presence of SDS

peptide mapping, 2 peptide fragments are Leu76-Pro-Gly-Ala-Leu-Ser-Glu82 and Lys94-Leu-His-Val-Asp-Pro-Glu100

bovine insulin + H2O

bovine insulin peptide fragments

708886

mass spectrometric identification, detailed overview

bovine myelin basic protein + H2O

Thermoactinomyces sp. CDF

Bovine serum albumin + H2O

752559

carboxymethylated yeast alcohol dehydrogenase + H2O

81314

5 different peptides containing the residues 5-13. 14-19, 68-77, 102-104, 105-108

81314

casein + H2O

8 entries

CXCR4-T140 + H2O

T140 photolabeled CXCR4, a G-protein-coupled receptor, containing the photoreactive amino acid 4-benzoyl-L-phenylalanine, Bpa, in positions 5 or 10. V8 protease digestion of both CXCR4/125I-[Bpa5]T140 and CXCR4/125I-[Bpa10]T140 adducts generates a fragment of 6 kDa suggesting that the T140 photoanalogs labeled a fragment corresponding to Lys154-Glu179 of the receptors 4th transmembrane domain

707448

equine beta-casein + H2O

equine beta-casein peptide fragments

Gelatin + H2O

Glucagon + H2O

GluV8 + H2O

degradation of the C-terminus at the Glu279-Asp280 bond is suspected to be a result from autoproteolysis

679805

38 kDa species

glycosylated bovine insulin + H2O

glycosylated bovine insulin peptide fragments

three differently glycosylated substrate forms

708886

mass spectrometric identification, detailed overview

hemocyanin + H2O

peptide fragments

hydrolysis of 2 isozymes of hemocyanin KLH1 and KLH2 from shellfish Megatura crenulata at Glu-Xaa and Asp-Xaa bonds

Hemoglobin + H2O

human hemoglobin + H2O

slpicedon consisting of a flanking region FR1, the EALER sequence, and a flanking region FR2, splicing reaction at E30-R31, facilitated by organic co-solvent-induced secondary conformation of alpha17-40 within which the sequence EALER plays a major role

653793

human parathyroid hormone(13-34) + H2O

peptides

insulin + H2O

insulin A-chain + H2O

peptide fragments

hydrolysis of Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12

insulin B-chain + H2O

peptide fragments

hydrolysis of Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20

insulin-like growth factor binding protein-1 + H2O

insulin-like growth factor binding protein-1 peptide fragments

from human decidual cells during gestation. The phosphorylation state influences the propensity of IGFBP-1 to proteolysis, overview. Generation of Glu C peptides by V8 protease, overview

708488

identification of Glu C peptides, overview

L-Phe-L-Leu-L-Glu-4-nitroanilide + H2O

L-Phe-L-Leu-L-Glu + 4-nitroaniline

Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O