Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
expression in Escherichia coli | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
S150A | inactive | Escherichia coli |
S150A | inactive | Providencia stuartii |
S150A | inactive | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
FL-casein | pH 6.0, 3°C | Providencia stuartii | |
0.0025 | - |
FL-casein | pH 6.0, 3°C | Escherichia coli | |
0.0039 | - |
CyPet-TatA-YPet | K0.5 value, Hill coefficient 1.8, pH 6.0, 3°C | Providencia stuartii | |
0.0065 | - |
FL-casein | pH 6.0, 3°C | Haemophilus influenzae | |
0.0076 | - |
TatA | K0.5 value, Hill coefficient 1.7, pH 6.0, 3°C | Providencia stuartii | |
0.022 | - |
TatA | K0.5 value, Hill coefficient 1.2, pH 6.0, 3°C | Escherichia coli | |
0.119 | - |
TatA | K0.5 value, Hill coefficient 1.2, pH 6.0, 3°C | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Haemophilus influenzae | P44783 | - |
- |
Haemophilus influenzae ATCC 51907 | P44783 | - |
- |
Providencia stuartii | P46116 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CyPet-TatA-YPet + H2O | engineered substrate based on transmembrane substrate TatA from Providencia stuartii, suitable for FRET assay | Providencia stuartii | ? | - |
? | |
FL-casein + H2O | - |
Escherichia coli | ? | - |
? | |
FL-casein + H2O | - |
Providencia stuartii | ? | - |
? | |
FL-casein + H2O | - |
Haemophilus influenzae | ? | - |
? | |
FL-casein + H2O | - |
Haemophilus influenzae ATCC 51907 | ? | - |
? | |
additional information | rhomboids may have two different mechanisms for substrate recognition. The transmembrane substrate is recognized on the hydrophobic belt of the enzyme by the exosite, which facilitates the substrate entry laterally into the active site. Soluble substrates, such as FL-casein, do not require initial exosite binding and approach the active site from the soluble face of the enzyme via the opening of loop 5 | Escherichia coli | ? | - |
? | |
additional information | rhomboids may have two different mechanisms for substrate recognition. The transmembrane substrate is recognized on the hydrophobic belt of the enzyme by the exosite, which facilitates the substrate entry laterally into the active site. Soluble substrates, such as FL-casein, do not require initial exosite binding and approach the active site from the soluble face of the enzyme via the opening of loop 5 | Providencia stuartii | ? | - |
? | |
additional information | rhomboids may have two different mechanisms for substrate recognition. The transmembrane substrate is recognized on the hydrophobic belt of the enzyme by the exosite, which facilitates the substrate entry laterally into the active site. Soluble substrates, such as FL-casein, do not require initial exosite binding and approach the active site from the soluble face of the enzyme via the opening of loop 5 | Haemophilus influenzae | ? | - |
? | |
additional information | rhomboids may have two different mechanisms for substrate recognition. The transmembrane substrate is recognized on the hydrophobic belt of the enzyme by the exosite, which facilitates the substrate entry laterally into the active site. Soluble substrates, such as FL-casein, do not require initial exosite binding and approach the active site from the soluble face of the enzyme via the opening of loop 5 | Haemophilus influenzae ATCC 51907 | ? | - |
? | |
TatA + H2O | transmembrane substrate from Providencia stuartii. Binding of TatA occurs with positive cooperativity in an exosite-mediated mode of substrate binding. Exosite formation is dependent on the oligomeric state of rhomboids, and when dimers are dissociated, allosteric substrate activation is not observed | Escherichia coli | ? | - |
? | |
TatA + H2O | transmembrane substrate from Providencia stuartii. Binding of TatA occurs with positive cooperativity in an exosite-mediated mode of substrate binding. Exosite formation is dependent on the oligomeric state of rhomboids, and when dimers are dissociated, allosteric substrate activation is not observed | Providencia stuartii | ? | - |
? | |
TatA + H2O | transmembrane substrate from Providencia stuartii. Binding of TatA occurs with positive cooperativity in an exosite-mediated mode of substrate binding. Exosite formation is dependent on the oligomeric state of rhomboids, and when dimers are dissociated, allosteric substrate activation is not observed | Haemophilus influenzae | ? | - |
? | |
TatA + H2O | transmembrane substrate from Providencia stuartii. Binding of TatA occurs with positive cooperativity in an exosite-mediated mode of substrate binding. Exosite formation is dependent on the oligomeric state of rhomboids, and when dimers are dissociated, allosteric substrate activation is not observed | Haemophilus influenzae ATCC 51907 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AarA | - |
Providencia stuartii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
TatA | pH 6.0, 3°C | Escherichia coli | |
0.0028 | - |
CyPet-TatA-YPet | pH 6.0, 3°C | Providencia stuartii | |
0.018 | - |
TatA | pH 6.0, 3°C | Providencia stuartii | |
0.06 | - |
FL-casein | pH 6.0, 3°C | Providencia stuartii | |
0.062 | - |
FL-casein | pH 6.0, 3°C | Escherichia coli | |
0.063 | - |
FL-casein | pH 6.0, 3°C | Haemophilus influenzae | |
0.07 | - |
TatA | pH 6.0, 3°C | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.5 | - |
Escherichia coli |
5.7 | 6.5 | - |
Providencia stuartii |
5.7 | 6.5 | - |
Haemophilus influenzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
TatA | pH 6.0, 3°C | Escherichia coli | |
0.59 | - |
TatA | pH 6.0, 3°C | Haemophilus influenzae | |
0.7 | - |
CyPet-TatA-YPet | pH 6.0, 3°C | Providencia stuartii | |
2.37 | - |
TatA | pH 6.0, 3°C | Providencia stuartii | |
2.48 | - |
FL-casein | pH 6.0, 3°C | Escherichia coli | |
9.7 | - |
FL-casein | pH 6.0, 3°C | Haemophilus influenzae | |
33 | - |
FL-casein | pH 6.0, 3°C | Providencia stuartii |