BRENDA - Enzyme Database show
show all sequences of 3.4.17.6

Bacterial peptidases. 3. An enzyme specific for N-acyl linkages to alanine

Levy, C.C.; Goldman, P.; J. Biol. Chem. 244, 4467-4472 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Benzoyl-Ala
at least 30% inhibition at 0.3 mM; competitive, Ki: 0.009 mM
Soil bacterium
benzoyl-Leu-Ala
at least 30% inhibition at 0.3 mM
Soil bacterium
carboxyl-terminal Ala containing compounds
-
Soil bacterium
Gly-Ala
non-competitive, Ki: 0.13 mM
Soil bacterium
His-Ala
non-competitive, Ki: 0.34 mM
Soil bacterium
additional information
not: compounds containing carboxyl-terminal Met or Gly
Soil bacterium
Phe-Ala
at least 30% inhibition at 0.3 mM
Soil bacterium
phosphate buffer
-
Soil bacterium
Tyr-Ala
at least 30% inhibition at 0.3 mM
Soil bacterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3
-
N-Benzoyl-Ala
-
Soil bacterium
4.3
-
N-benzoyl-L-aminobutyric acid
-
Soil bacterium
6.7
-
N-benzoyl-Gly
-
Soil bacterium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect: Co2+, Mn2+, Mg2+, Fe3+, Fe2+, Cu2+, Cd2+, Ni2+
Soil bacterium
Zn2+
required
Soil bacterium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-aminobenzoylalanine + H2O
Soil bacterium
-
?
-
-
-
peptidyl-L-alanine + H2O
Soil bacterium
peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Soil bacterium
-
unidentified
-
Purification (Commentary)
Commentary
Organism
-
Soil bacterium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.3
-
-
Soil bacterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-amino-4-deoxypteroyl-Ala + H2O
-
81311
Soil bacterium
4-amino-4-deoxypteroate + Ala
-
81311
Soil bacterium
ir
4-aminobenzoyl-Ala + H2O
-
81311
Soil bacterium
4-aminobenzoate + Ala
-
81311
Soil bacterium
ir
4-aminobenzoylalanine + H2O
-
81311
Soil bacterium
?
-
-
-
-
acyl-Ala + H2O
-
81311
Soil bacterium
? + Ala
-
81311
Soil bacterium
ir
Ala-Ala + H2O
-
81311
Soil bacterium
Ala + Ala
-
81311
Soil bacterium
ir
benzoyl-Ala + H2O
-
81311
Soil bacterium
benzoate + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ala + H2O
-
81311
Soil bacterium
benzyloxy-CHO + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ala-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Ala + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Asp-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Asp + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ile-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Ile + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Leu-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Leu + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Met-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Met + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Phe-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Phe + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ser-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Ser + Ala
-
81311
Soil bacterium
ir
Glu-Ala + H2O
-
81311
Soil bacterium
Glu + Ala
-
81311
Soil bacterium
ir
Ile-Ala + H2O
-
81311
Soil bacterium
Ile + Ala
-
81311
Soil bacterium
ir
Leu-Ala + H2O
-
81311
Soil bacterium
Leu + Ala
-
81311
Soil bacterium
ir
Met-Ala + H2O
-
81311
Soil bacterium
Met + Ala
-
81311
Soil bacterium
ir
N-benzoyl-Gly-Ala + H2O
-
81311
Soil bacterium
N-benzoyl-Gly + Ala
-
-
-
-
N-benzoyl-L-aminobutyric acid + H2O
-
81311
Soil bacterium
benzoate + L-aminobutyric acid
-
-
-
-
peptidyl-L-alanine + H2O
peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
81311
Soil bacterium
?
-
-
-
-
Phe-Ala + H2O
-
81311
Soil bacterium
Phe + Ala
-
81311
Soil bacterium
ir
Ser-Ala + H2O
-
81311
Soil bacterium
Ser + Ala
-
81311
Soil bacterium
ir
Tyr-Ala + H2O
-
81311
Soil bacterium
Tyr + Ala
-
81311
Soil bacterium
ir
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Soil bacterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Soil bacterium
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.009
-
Benzoyl-Ala
-
Soil bacterium
0.13
-
Gly-Ala
non-competitive
Soil bacterium
0.34
-
His-Ala
non-competitive
Soil bacterium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Benzoyl-Ala
at least 30% inhibition at 0.3 mM; competitive, Ki: 0.009 mM
Soil bacterium
benzoyl-Leu-Ala
at least 30% inhibition at 0.3 mM
Soil bacterium
carboxyl-terminal Ala containing compounds
-
Soil bacterium
Gly-Ala
non-competitive, Ki: 0.13 mM
Soil bacterium
His-Ala
non-competitive, Ki: 0.34 mM
Soil bacterium
additional information
not: compounds containing carboxyl-terminal Met or Gly
Soil bacterium
Phe-Ala
at least 30% inhibition at 0.3 mM
Soil bacterium
phosphate buffer
-
Soil bacterium
Tyr-Ala
at least 30% inhibition at 0.3 mM
Soil bacterium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.009
-
Benzoyl-Ala
-
Soil bacterium
0.13
-
Gly-Ala
non-competitive
Soil bacterium
0.34
-
His-Ala
non-competitive
Soil bacterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3
-
N-Benzoyl-Ala
-
Soil bacterium
4.3
-
N-benzoyl-L-aminobutyric acid
-
Soil bacterium
6.7
-
N-benzoyl-Gly
-
Soil bacterium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect: Co2+, Mn2+, Mg2+, Fe3+, Fe2+, Cu2+, Cd2+, Ni2+
Soil bacterium
Zn2+
required
Soil bacterium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-aminobenzoylalanine + H2O
Soil bacterium
-
?
-
-
-
peptidyl-L-alanine + H2O
Soil bacterium
peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Soil bacterium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.3
-
-
Soil bacterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-amino-4-deoxypteroyl-Ala + H2O
-
81311
Soil bacterium
4-amino-4-deoxypteroate + Ala
-
81311
Soil bacterium
ir
4-aminobenzoyl-Ala + H2O
-
81311
Soil bacterium
4-aminobenzoate + Ala
-
81311
Soil bacterium
ir
4-aminobenzoylalanine + H2O
-
81311
Soil bacterium
?
-
-
-
-
acyl-Ala + H2O
-
81311
Soil bacterium
? + Ala
-
81311
Soil bacterium
ir
Ala-Ala + H2O
-
81311
Soil bacterium
Ala + Ala
-
81311
Soil bacterium
ir
benzoyl-Ala + H2O
-
81311
Soil bacterium
benzoate + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ala + H2O
-
81311
Soil bacterium
benzyloxy-CHO + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ala-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Ala + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Asp-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Asp + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ile-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Ile + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Leu-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Leu + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Met-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Met + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Phe-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Phe + Ala
-
81311
Soil bacterium
ir
benzyloxycarbonyl-Ser-Ala + H2O
-
81311
Soil bacterium
benzyloxycarbonyl-Ser + Ala
-
81311
Soil bacterium
ir
Glu-Ala + H2O
-
81311
Soil bacterium
Glu + Ala
-
81311
Soil bacterium
ir
Ile-Ala + H2O
-
81311
Soil bacterium
Ile + Ala
-
81311
Soil bacterium
ir
Leu-Ala + H2O
-
81311
Soil bacterium
Leu + Ala
-
81311
Soil bacterium
ir
Met-Ala + H2O
-
81311
Soil bacterium
Met + Ala
-
81311
Soil bacterium
ir
N-benzoyl-Gly-Ala + H2O
-
81311
Soil bacterium
N-benzoyl-Gly + Ala
-
-
-
-
N-benzoyl-L-aminobutyric acid + H2O
-
81311
Soil bacterium
benzoate + L-aminobutyric acid
-
-
-
-
peptidyl-L-alanine + H2O
peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
81311
Soil bacterium
?
-
-
-
-
Phe-Ala + H2O
-
81311
Soil bacterium
Phe + Ala
-
81311
Soil bacterium
ir
Ser-Ala + H2O
-
81311
Soil bacterium
Ser + Ala
-
81311
Soil bacterium
ir
Tyr-Ala + H2O
-
81311
Soil bacterium
Tyr + Ala
-
81311
Soil bacterium
ir
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Soil bacterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Soil bacterium
Other publictions for EC 3.4.17.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
81313
Miyagawa
-
Purification and properties of ...
Rhodococcus hoagii
Agric. Biol. Chem.
50
1527-1531
1986
-
-
-
-
-
-
10
-
-
3
2
-
-
1
-
-
1
-
-
-
1
-
10
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
3
2
-
-
-
-
1
-
-
1
-
10
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
81312
Iwaya
Simultaneous deletion of D-ala ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
74
2980-2984
1977
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
81311
Levy
Bacterial peptidases. 3. An en ...
Soil bacterium
J. Biol. Chem.
244
4467-4472
1969
-
-
-
-
-
-
9
3
-
2
-
2
-
1
-
-
1
-
-
-
1
-
24
-
1
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
9
3
3
-
2
-
2
-
-
-
1
-
-
1
-
24
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-