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EC Tree
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Release of a C-terminal alanine from a peptide or a variety of pteroyl or acyl groups
Synonyms
D-Ala carboxypeptidase IB-C, EC 3.4.12.11, N-benzoyl-L-alanine-aminohydrolase,
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D-Ala carboxypeptidase IB-C
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EC 3.4.12.11
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formerly
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N-benzoyl-L-alanine-aminohydrolase
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Release of a C-terminal alanine from a peptide or a variety of pteroyl or acyl groups
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hydrolysis of peptide bond
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exopeptidase, C-terminus, amino acid
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4-amino-4-deoxypteroyl-Ala + H2O
4-amino-4-deoxypteroate + Ala
Soil bacterium
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ir
4-aminobenzoyl-Ala + H2O
4-aminobenzoate + Ala
Soil bacterium
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ir
4-aminobenzoylalanine + H2O
?
Soil bacterium
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?
acyl-Ala + H2O
? + Ala
Soil bacterium
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ir
Ala-Ala + H2O
Ala + Ala
Soil bacterium
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ir
benzoyl-Ala + H2O
benzoate + Ala
benzyloxycarbonyl-Ala + H2O
benzyloxy-CHO + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Ala-Ala + H2O
benzyloxycarbonyl-Ala + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Asp-Ala + H2O
benzyloxycarbonyl-Asp + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Ile-Ala + H2O
benzyloxycarbonyl-Ile + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Leu-Ala + H2O
benzyloxycarbonyl-Leu + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Met-Ala + H2O
benzyloxycarbonyl-Met + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Phe-Ala + H2O
benzyloxycarbonyl-Phe + Ala
Soil bacterium
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ir
benzyloxycarbonyl-Ser-Ala + H2O
benzyloxycarbonyl-Ser + Ala
Soil bacterium
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ir
Glu-Ala + H2O
Glu + Ala
Soil bacterium
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ir
Ile-Ala + H2O
Ile + Ala
Soil bacterium
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ir
Leu-Ala + H2O
Leu + Ala
Soil bacterium
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ir
Met-Ala + H2O
Met + Ala
Soil bacterium
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ir
N-benzoyl-Gly-Ala + H2O
N-benzoyl-Gly + Ala
N-benzoyl-L-aminobutyric acid + H2O
benzoate + L-aminobutyric acid
p-chlorobenzoyl-Ala + H2O
p-chlorobenzoate + Ala
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ir
p-chlorobenzoyl-Gly + H2O
p-chlorobenzoate + Gly
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ir
p-hydroxybenzoyl-Ala + H2O
p-hydroxybenzoate + Ala
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ir
p-methoxybenzoyl-Gly + H2O
p-methoxybenzoate + Gly
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ir
p-methoxyenzoyl-Ala + H2O
p-methoxybenzoate + Ala
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ir
p-nitrobenzoyl-Ala + H2O
p-nitrobenzoate + Ala
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ir
p-nitrobenzoyl-Gly + H2O
p-nitrobenzoate + Gly
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ir
peptidyl-L-alanine + H2O
?
Soil bacterium
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peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
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?
Phe-Ala + H2O
Phe + Ala
Soil bacterium
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ir
Ser-Ala + H2O
Ser + Ala
Soil bacterium
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ir
Tyr-Ala + H2O
Tyr + Ala
Soil bacterium
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ir
benzoyl-Ala + H2O
benzoate + Ala
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ir
benzoyl-Ala + H2O
benzoate + Ala
Soil bacterium
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ir
N-benzoyl-Gly-Ala + H2O
N-benzoyl-Gly + Ala
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ir
N-benzoyl-Gly-Ala + H2O
N-benzoyl-Gly + Ala
Soil bacterium
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?
N-benzoyl-L-aminobutyric acid + H2O
benzoate + L-aminobutyric acid
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ir
N-benzoyl-L-aminobutyric acid + H2O
benzoate + L-aminobutyric acid
Soil bacterium
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?
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4-aminobenzoylalanine + H2O
?
Soil bacterium
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?
peptidyl-L-alanine + H2O
?
Soil bacterium
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peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
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?
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Co2+
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146% activation at 0.5 mM, optimum concentration
Mg2+
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can partially replace cobalt
Ni2+
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can partially replace cobalt
Zn2+
Soil bacterium
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required
additional information
Soil bacterium
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no effect: Co2+, Mn2+, Mg2+, Fe3+, Fe2+, Cu2+, Cd2+, Ni2+
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1,10-phenanthroline
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100% inhibition at 1 mM
2-2'-dipyridyl
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80% inhibition at 1 mM
8-hydroxyquinoline
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100% inhibition at 1 mM
Ag2+
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100% inhibition at 1 mM
Benzoyl-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM; competitive, Ki: 0.009 mM
benzoyl-Leu-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM
carboxyl-terminal Ala containing compounds
Soil bacterium
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Cu2+
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100% inhibition at 1 mM
Fe2+
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33% inhibition at 1 mM
Fe3+
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47% inhibition at 1 mM
Gly-Ala
Soil bacterium
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non-competitive, Ki: 0.13 mM
Hg2+
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100% inhibition at 1 mM
His-Ala
Soil bacterium
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non-competitive, Ki: 0.34 mM
iodoacetic acid
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25% inhibition at 1 mM
PCMB
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100% inhibition at 1 mM
Phe-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM
phosphate buffer
Soil bacterium
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Tyr-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM
additional information
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simultaneous mutational deletion of enzyme activity and penicillin-binding component IV
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additional information
Soil bacterium
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not: compounds containing carboxyl-terminal Met or Gly
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4.3
N-Benzoyl-Ala
Soil bacterium
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6.7
N-benzoyl-Gly
Soil bacterium
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4.3
N-benzoyl-L-aminobutyric acid
Soil bacterium
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0.009
Benzoyl-Ala
Soil bacterium
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0.13
Gly-Ala
Soil bacterium
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non-competitive
0.34
His-Ala
Soil bacterium
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non-competitive
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additional information
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activities per g wet weight 10fold higher in wild-type than in K12 cells
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30
Soil bacterium
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assay at
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mutant K12 with decreased activity
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brenda
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brenda
Soil bacterium
unidentified
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brenda
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CBAL_GEOSE
30
0
3367
Swiss-Prot
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40000
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6 * 40000, SDS-PAGE
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hexamer
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6 * 40000, SDS-PAGE
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Soil bacterium
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medicine
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possible penicillin target
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Levy, C.C.; Goldman, P.
Bacterial peptidases. 3. An enzyme specific for N-acyl linkages to alanine
J. Biol. Chem.
244
4467-4472
1969
Soil bacterium
brenda
Iwaya, M.; Strominger, J.L.
Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12
Proc. Natl. Acad. Sci. USA
74
2980-2984
1977
Escherichia coli
brenda
Miyagawa, E.; Harada, T.; Motoki, Y.
Purification and properties of N-benzoyl-L-alanine aminohydrolase from Corynebacterium equi
Agric. Biol. Chem.
50
1527-1531
1986
Rhodococcus equi
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brenda
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3.4.17.6 alanine carboxypeptidase
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