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Literature summary for 3.4.17.12 extracted from

  • Reverter, D.; Maskos, K.; Tan, F.; Skidgel, R.A.; Bode, W.
    Crystal structure of human carboxypeptidase M, a membrane-bound enzyme that regulates peptide hormone activity (2004), J. Mol. Biol., 338, 257-269.
    View publication on PubMed
Search for more literature for 3.4.17.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of 3'-truncated, glycosylphosphatidylinositol-free, soluble enzyme in insect cells using the baculovirus transfection system Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified, recombinant, glycosylphosphatidylinositol-free enzyme, X-ray diffraction structure determination and analysis at 3.0 A resolution, modelling Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Homo sapiens 9986
-
extracellular
-
 Homo sapiens
-
-
plasma membrane anchored via glycosylphosphatidylinositol Homo sapiens 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, recombinant 3-truncated, glycosylphosphatidylinositol-free enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14384
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation at Asn89 and partially at Asn21 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant 3'-truncated, glycosylphosphatidylinositol-free, soluble enzyme from insect cells by anion exchange chromatography and gel filtration to homogeneity Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
cleavage of C-terminal arginine or lysine residues from polypeptides catalytic mechanism, active site groove structure involving residues Y242, W241, K228, K269, E264, R127, H66, E69, and H173, substrate interactins Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information wide tissue distribution Homo sapiens
-
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, recombinant 3'-truncated, glycosylphosphatidylinositol-free enzyme, SDS-PAGE Homo sapiens
More catalytic domain structure Homo sapiens

Synonyms

Synonyms Comment Organism
CPM
-
 Homo sapiens